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Method of cryopreserving selected sperm cells

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12N-009/24
출원번호 UP-0608079 (2006-12-07)
등록번호 US-7820425 (2010-11-15)
발명자 / 주소
  • Schenk, John L.
출원인 / 주소
  • XY, LLC
대리인 / 주소
    Miles, Craig R.
인용정보 피인용 횟수 : 5  인용 특허 : 467

초록

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any num

대표청구항

What is claimed is: 1. A method of freezing separated sperm cells, comprising the steps of: a. obtaining sperm cells from a male of a species of non-human mammal; b. differentiating said sperm cells based upon at least one property which allows a portion of said sperm cells to be identified as bear

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  184. Kashima Shingo (Hachiouji JPX), Illuminating optical system.
  185. Spangenberg Peter, Illuminator optical assembly for an analytical instrument and methods of alignment and manufacture.
  186. David A. Basiji ; William E. Ortyn, Imaging and analyzing parameters of small moving objects such as cells.
  187. Ortyn, William E.; Basiji, David A., Imaging and analyzing parameters of small moving objects such as cells in broad flat flow.
  188. Kosaka Tokihiro (Kakogawashi JPX), Imaging flow cytometer for imaging and analyzing particle components in a liquid sample.
  189. Kreikebaum Gerhard (San Bernardino CA) Chandler David L. (Highland CA), Improved particle sensor and method for assaying a particle.
  190. Ross, III, Denwood F.; Dolan, Mary L.; Raja, Ranganath R.; Rice, Brian G.; Walker, Craig W.; Kappel, David; Fischer, Robert E.; Kindt-Larsen, Ture, Inspection of ophthalmic lenses using absorption.
  191. Cassou Robert (Rue Clemenceau 61300 L\Aigle FRX) Cassou Bertrand (Saint Symphorien des Bruyeres 61300 L\Aigle FRX) Cassou Maurice (Rue Clemenceau 61300 L\Aigle FRX), Instrument for artificial insemination, embryo transfer or sampling follicular liquids in mammals.
  192. W. Peter Hansen, Instrument for selecting and depositing multicellular organisms and other large objects.
  193. Quake, Stephen R.; Chou, Hou-Pu, Integrated active flux microfluidic devices and methods.
  194. Abergel, Aline; Garcia, Firmin, Integrated pump dispenser.
  195. Sommer Holger T. (Greenbelt MD), Integrating sphere aerosol particle detector.
  196. Johnston Roger G. (Los Alamos NM), Interferometric apparatus and method for detection and characterization of particles using light scattered therefrom.
  197. Chandler Van S., Interlaced lasers for multiple fluorescence measurement.
  198. Gourley Paul L. (12508 Loyola ; NE. Albuquerque NM 87112) Gourley Mark F. (7509 Spring Lake Dr. ; Apt. B1 Bethesda MD 20817), Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells.
  199. Bova G. Steven, Laser cell purification system.
  200. Martin John C. (Los Alamos NM) Buican Tudor N. (Los Alamos NM), Laser particle sorter.
  201. Ichie Koji,JPX, Laser scanning optical system and laser scanning optical apparatus.
  202. Eisenlauer Josef (Ludwigshafen DEX) Horn Dieter (Heidelberg DEX) Ditter Walter (Heidelberg DEX) Eipel Heinz (Bensheim DEX), Laser-optical arrangement for measuring the degree of dispersion in flowing systems.
  203. Ishikawa Masanori,JPX, Lens barrel.
  204. Unterleitner Fred C. (Palo Alto CA), Light beam focal spot elongation in flow cytometry devices.
  205. Aughton John E. (London GB2), Light beam splitter.
  206. Watson James V. (Great Shelford GBX), Light collecting device with chamber including ellipsoidal surface and spherical surface.
  207. Gjelsnes Oddbj.o slashed.rn,NOX ; Tangen Reidar,NOX, Liquid flow cytometer.
  208. Buchanan, Kris S.; Herickhoff, Lisa A., Low acceleration method of flow cytometry.
  209. Kenyon Bruce A. (Sumner WA), Low backscatter illumination system.
  210. Hanninen Pekka,FIX ; Hell Stefan,DEX, Luminescence-scanning microscopy process and a luminescence scanning microscope utilizing picosecond or greater pulse la.
  211. Vaez-Iravani Mehdi, Massively parallel inspection and imaging system.
  212. Carleton Joseph G. (Palo Alto CA) Sweet Richard G. (Palo Alto CA), Measurement of angular dependence of scattered light in a flowing stream.
  213. Wells David (Arlington VA), Measurement of concentrations of dissolved solvent.
  214. Jalink, Kees, Membrane molecule indicator compositions and methods.
  215. Spangenberg Peter E., Method and apparatus for an optical illuminator assembly and its alignment.
  216. Zld Tibor (PhilippiStr. 13 4400 Mnster DEX), Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer.
  217. Quake Stephen ; Volkmuth Wayne D., Method and apparatus for analysis and sorting of polynucleotides based on size.
  218. Sizto Ning L. (Freemont CA) Dietz Louis J. (Mountain View CA), Method and apparatus for cell counting and cell classification.
  219. Sizto Ning L. ; Dietz Louis J., Method and apparatus for cell counting and cell classification.
  220. Miers David R., Method and apparatus for detecting scattered light in an analytical instrument.
  221. Yamashita Mikio (Ibaraki JPX) Sato Takuzo (Ibaraki JPX) Noguchi Yoshio (Matsudo JPX) Uchibori Yoshinobu (Ibaraki JPX) Shinohara Akio (Fujisawa JPX) Soga Hiroshi (Yamato JPX) Miyazaki Sadayuki (Tokyo , Method and apparatus for discriminating minute particles.
  222. Baker Jeffrey P. (Poway CA) Mott Steven C. (San Diego CA) Wright Craig A. (San Diego CA), Method and apparatus for dry particle analysis.
  223. Hogg Walter R. (Miami Lakes FL), Method and apparatus for ejecting a metered amount of particulate sample.
  224. Zld Tibor (Philippistrasse 13 4400 Mnster DEX), Method and apparatus for fast, reliable, and environmentally safe dispensing of fluids, gases and individual particles o.
  225. Malachowski George C., Method and apparatus for flow cytometry.
  226. Malachowski, George C., Method and apparatus for flow cytometry.
  227. Becker, Frederick F.; Gascoyne, Peter R. C.; Huang, Ying; Wang, Xiao-Bo; Yang, Jun, Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation.
  228. Taboada John (San Antonio TX) Altschuler Bruce R. (Dayton OH), Method and apparatus for generating a structured light beam array.
  229. Oetliker Hans (Muri CHX) Winiger Peter (Wohlen CHX) Stempeel Sonia (Zurich CHX), Method and apparatus for guiding and collecting light in photometry or the like.
  230. Wieder Irwin (Los Altos CA), Method and apparatus for improved analytical fluorescent spectroscopy.
  231. Horiuchi Hideyuki,JPX, Method and apparatus for particle image analysis using flow cell.
  232. Lombardo Igino (Sharon MA) Hansen W. Peter (Middleboro MA), Method and apparatus for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting dev.
  233. Pomeroy Patrick C. (1920 Gilly La. Concord CA 94518) Madsen Randall (8970 Taurus Pl. San Diego CA 92126) Chan Wai P. (18845 California St. Castro Valley CA 94546) Lemke Gary (1630 St. David Dr. Danvi, Method and apparatus for post-transfer assaying of material on solid support.
  234. Palsson, Bernhard O.; Koller, Manfred R.; Eisfeld, Timothy M., Method and apparatus for selectively targeting specific cells within a cell population.
  235. Palsson, Bernhard O.; Koller, Manfred R.; Eisfeld, Timothy M., Method and apparatus for selectively targeting specific cells within a mixed cell population.
  236. Graham Marshall Donnie, Method and apparatus for sensing and characterizing particles.
  237. Ghde Hildegard (Nottuln DEX) Schumann Johannes (Mnster DEX), Method and apparatus for sorting particles.
  238. North ; Jr. Howard L. (Los Gatos CA), Method and apparatus for sorting particles with a moving catcher tube.
  239. Ellwart Joachim,DEX ; Karls Ingolf,DEX, Method and arrangement for monitoring a separating liquid stream.
  240. Fulton R. Jerrold, Method and compositions for flow cytometric determination of DNA sequences.
  241. Maltsev Valeri Pavlovich (Novosibirsk RUX) Chernyshev Andrei Vitalievich (Novosibirsk RUX), Method and device for determination of parameters of individual microparticles.
  242. Zold Tibor (Schmale Strasse 19 44 Munster DEX), Method and device for sorting particles suspended in an electrolyte.
  243. Bryant Bernard J. (509 Scripps Dr. Davis CA 95616), Method and material for increasing the percentage of mammalian offspring of either sex.
  244. Recktenwald Diether J. (Cupertino CA) Kerndt Rickie S. (Cupertino CA) Loken Michael R. (Los Altos CA) Chen Chia H. (San Jose CA), Method and materials for calibrating flow cytometers and other analysis instruments.
  245. Recktenwald Diether J. (Cupertino CA) Kerndt Rickie S. (Cupertino CA) Loken Michael R. (Los Altos CA) Chen Chia H. (San Jose CA), Method and materials for calibrating flow cytometers and other analysis instruments.
  246. Shrimpton Wallace (320 Judah St. San Francisco CA 94122), Method and means for controlling the sex of mammalian offspring and product therefor.
  247. Shrimpton Wallace (320 Judah St. San Francisco CA 94122), Method and means for controlling the sex of mammalian offspring and product therefor.
  248. Sweet Richard G. (Palo Alto CA) Moore Wayne A. (San Francisco CA) Nozaki ; Jr. Tom (Palo Alto CA) Stovel Richard T. (Palo Alto CA), Method and means for delivering liquid samples to a sample scanning device.
  249. Price Jeffrey H. ; Gough David A., Method and means of least squares designed filters for image segmentation in scanning cytometry.
  250. Rossi John J. (Glendora CA), Method for amplification and detection of RNA and DNA sequences.
  251. Lombardo Igino (Sharon MA) Barry Donald E. (Norwood MA), Method for automatically setting the correct phase of the charge pulses in an electrostatic flow sorter.
  252. Schulte Harvey (Los Altos CA) Noto Sal (Sunnyvale CA), Method for causing vortices in a test tube.
  253. Brigitte Walderich DE; Renate Nordin DE, Method for cryoconservation of Zebrafish sperm.
  254. Lombardo Igino (Sharon MA) Barry Donald E. (Norwood MA) Hansen W. Peter (Middleboro MA), Method for detecting and controlling flow rates of the droplet forming stream of an electrostatic particle sorting appar.
  255. Terstappen Leon W. M. M. (Los Altos CA) Loken Michael R. (Palo Alto CA) Shah Virendra O. (Santa Clara CA), Method for discriminating between intact and damaged cells in a sample.
  256. Corio Mark A. ; Leary James F., Method for flexibly sorting particles.
  257. Nimrod Beatrice (24 Wallace St. New Britain CT 06051), Method for guide-wire placement and novel syringe therefor.
  258. Koller, Manfred R.; Palsson, Bernhard O.; Eisfeld, Timothy M., Method for inducing a response in one or more targeted cells.
  259. Kasper Gerhard (Downers Grove IL) Wen Horng Y. (Brookfield IL), Method for measurement of impurities in liquids.
  260. Ito Yuji (Chigasaki JPX) Saito Atsushi (Yokohama JPX), Method for measuring a specimen by the use of fluorescent light.
  261. Barry Donald E. (Norwood MA) Lombardo Igino (Sharon MA), Method for measuring the velocity of a perturbed jetting fluid in an electrostatic particle sorting system.
  262. Koenig, Karsten; Halbhuber, Karl-Juergen; Fischer, Peter; Riemann, Iris, Method for optical excitation of fluorophore marked DNA and RNA.
  263. Gill James E. ; Chupp Vernon L. ; Hove Luc Van, Method for performing automated analysis.
  264. Fulwyler Mack J. (Los Alamos NM) Hatcher C. William (Los Alamos NM), Method for producing uniform particles.
  265. Blecher Stan R.,CAX, Method for separating sex specific molecules and non-sex specific molecules.
  266. Benjamin Thomas L. ; Kohn Barbara ; Basker Christopher J. ; George Susan ; Livingston David, Method for sex determination of mammalian offspring.
  267. Thomas L. Benjamin ; Barbara Kohn ; Christopher J. Basker ; Susan George ; David Livingston, Method for sex determination of mammalian offspring.
  268. Hnninen Pekka (Korkeavuorenkatu 2 B 25 FIN-20100 Turku FIX) Soini Erkki (Krypingintie 20 FIN-21610 Kirjala FIX), Method for the excitation of dyes.
  269. Wolleschensky, Ralf, Method for the optical acquisition of characteristic sizes of an illuminated sample.
  270. Dervan Peter B. ; Baird Eldon, Method for the synthesis of pyrrole and imidazole carboxamides on a solid support.
  271. Zavos Panayiotis M. (Lexington KY) Dawson Karl A. (Lexington KY), Method for x and y spermatozoa separation.
  272. Auer Robert E. (Miami FL), Method of and apparatus for detecting change in the breakoff point in a droplet generation system.
  273. Auer Robert E. (Miami FL), Method of and apparatus for detecting change in the breakoff point in a droplet generation system.
  274. Schenk,John L., Method of cryopreserving selected sperm cells.
  275. Mueller William A. (Dedham MA), Method of discriminating between dyed particles and background fluorescence of the dye.
  276. Ericsson Ronald J. (Sausalito CA), Method of increasing the incidence of female offspring.
  277. Gray ; Jr. Carl O. (14240 Fancher Rd. Johnstown OH 43031), Method of preserving spermatozoa.
  278. Adair Edwin L. (191 East Orchard Littleton CO 80123), Method of treating collected mammal semen and separating sperm into X Y components.
  279. Johnson Lawrence A. (Silver Spring MD), Method to preselect the sex of offspring.
  280. Critser John K. (Carmel IN) Gao D. Y. (Indianapolis IN), Method to quickly add cryoprotectants to sperm cells while maintaining viability.
  281. Ulmer Kevin M. (Cohasset MA), Methods and apparatus for DNA sequencing.
  282. Spangenberg Peter E., Methods and apparatus for an optical illuminator assembly and its alignment.
  283. Kamentsky Louis A. (180 Beacon St. ; #17G Boston MA 02116), Methods and apparatus for measuring multiple optical properties of biological specimens.
  284. Ellington Joanna E. ; Oliver Sylvia Adams, Methods and compositions to improve germ cell and embryo survival and function.
  285. Kenneth A. Davis, Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells.
  286. Loehrlein, Christine; Pollart, Dan; Shaler, Thomas; Stephens, Kathy; Tan, Yuping; Wong, Linda; Monforte, Joseph, Methods for analysis of gene expression.
  287. Gray Joe W. (Livermore CA) Pinkel Daniel (Walnut Creek CA), Methods for chromosome-specific staining.
  288. Spaulding Glenn F. (Chandler AZ), Methods for determining antibodies specific for sex associated sperm membrane proteins.
  289. Brown David B., Methods for diagnosing human male infertility.
  290. Seidel, George; Herickhoff, Lisa; Schenk, John, Methods for improving sheath fluids and collection systems for sex-specific cytometer sorting of sperm.
  291. Gray Joe W. ; Pinkel Daniel, Methods of biological dosimetry employing chromosome-specific staining.
  292. Gert Blankenstein DE, Micro-flow system for particle separation and analysis.
  293. Lievan Bonnie A., Microchip optical transport technology for use in a personal flow cytometer.
  294. Spence, Charles F.; Fu, Anne Y.; Quake, Stephen R.; Arnold, Frances H., Microfabricated cell sorter for chemical and biological materials.
  295. Bernhard H. Weigl ; Paul Yager ; James P. Brody ; Mark R. Holl ; Fred K. Forster ; Eric Altendorf ; Paul C. Galambos ; Margaret Kenny ; David Schutte ; Gregory Hixson ; Diane Zebert ; Andr, Microfabricated devices and methods.
  296. Weigl Bernhard H. ; Yager Paul ; Brody James P. ; Holl Mark R. ; Kenny Margaret ; Schutte David ; Hixson Gregory ; Zebert M. Diane ; Kamholz Andrew ; Wu Caicai ; Altendorf Eric, Microfabricated diffusion-based chemical sensor.
  297. Nelson, Robert J.; Hooper, Herbert H.; Hauser, Alan K.; Singh, Sharat; Williams, Stephen J.; Sassi, Alexander P., Microfluidic apparatus and method for purification and processing.
  298. Beebe David J. ; Glasgow Ian K. ; Wheeler Matthew B., Microfluidic embryo and/or oocyte handling device and method.
  299. Larsen Lawrence E. (308 Hamilton Ave. Silver Spring MD 20901), Millimeter wave flow cytometer.
  300. Uehara Makoto (Tokyo JPX) Hashimoto Sumio (Kawasaki JPX), Mirror converging-type illumination optical system.
  301. Barbetti Manlio (Rome ITX), Mixtures of FSH and LH from pig hypophyses in a definite ratio.
  302. Lary, Todd P.; Snow, Christopher W.; Riley, John S.; Burr, Robert C., Monitoring and control of droplet sorting.
  303. Norton Pierce Owen ; Hoffman Robert A., Multi-illumination-source flow particle analyzer with inter-location emissions crosstalk cancelation.
  304. Iketaki Yoshinori,JPX ; Fujii Masaaki,JPX, Multi-wavelength optical microscope.
  305. Ortyn, William E.; Basiji, David A., Multipass cavity for illumination and excitation of moving objects.
  306. Ortyn, William E.; Basiji, David A.; Frost, Keith L.; McRuer, N. Robert, Multipass cavity for illumination and excitation of moving objects.
  307. Dovichi Norman J. (Edmonton CAX) Zhang Jian Z. (Edmonton CAX), Multiple capillary biochemical analyzer.
  308. George Seidel ; Lisa Herickhoff ; John Schenk, Multiple sexed embryo production system for mammals.
  309. Van den Engh Ger (Seattle WA) Esposito Richard J. (Seattle WA), Multiple sort flow cytometer.
  310. Minott Peter O. (Bowie MD), Multiprism collimator.
  311. Masamitsu Haruna JP, Nanosecond gate spectroscopic diagnostic device.
  312. Connors Kenneth M. ; Monosov Anna, Native-state method and system for determining viability and proliferative capacity of tissues in vitro.
  313. Seul, Michael; Banerjee, Sukanta; Podual, Kairali, On-chip analysis of particles and fractionation of particle mixtures using light-controlled electrokinetic assembly of particles near surfaces.
  314. Ward Anthony J. (San Ramon CA) Mercolino Thomas J. (Pleasanton CA) Recktenwald Diether J. (Cupertino CA), One step method for detection and enumeration of absolute counts of one more cell populations in a sample.
  315. Price Jeffrey H. (3881 Camino Lindo San Diego CA 92122) Gough David (1435 Big Canyon Ter. Cardiff CA 92007), Operator independent image cytometer.
  316. Sharpe, Johnathan; Schaare, Peter Niven, Optical apparatus.
  317. Sharpe,Jonathan C.; Schaare,Peter N., Optical apparatus.
  318. Steen Harald (Wolffsgt. 3 N-0358 Oslo NOX), Optical arrangement for flow cytometer to facilitate large angle light-scattering measurement.
  319. Bergmann Ernest Eisenhardt, Optical beamsplitter.
  320. Fritz, Bernard Steven, Optical detection system for flow cytometry.
  321. Goshima Takeshi (Tokyo JA) Endo Kiyonobu (Yokohama JA), Optical device for producing a minute light beam.
  322. Gaucher Jean-Claude (le Val St. Gerwain FRX), Optical device with a high collection efficiency and cytofluorimeter making use of the same.
  323. Brunsden Barry S. (Chicago IL), Optical device with conical input and output prism faces.
  324. Sage Burton H. (Raleigh NC) Adrion Robert F. (Cary NC) Malpass Michael W. (Cary NC), Optical features of flow cytometry apparatus.
  325. Kusuzawa, Hideo; Imura, Yasuyuki; Kobayashi, Hironori; Ishisaka, Masaki, Optical information measuring apparatus.
  326. Ito Taku (Tokyo JPX), Optical multilayer thin film and beam splitter.
  327. Ito Taku (Tokyo JPX), Optical multilayer thin film and beam splitter.
  328. Fukuda Masakazu (Hyogo JPX) Nakamoto Hiroyuki (Hyogo JPX) Seshimo Hiroyuki (Hyogo JPX) Tohori Hidemichi (Hyogo JPX), Optical particle analyzing apparatus having two types of light source.
  329. Eidenschink Herrn H. (Gutenbergstr. 1 5609 Huckeswagen DEX) Eidenschink Marianne (Gutenbergstr. 1 5609 Huckeswagen DEX), Optical process and apparatus for determining particle size of colloidal solution.
  330. Roslaniec Mary C. ; Martin John C. ; Jett James H. ; Cram L. Scott, Optical selection and collection of DNA fragments.
  331. Kopf-Sill, Anne R.; Chow, Andrea W., Optimized high-throughput analytical system.
  332. Koller, Manfred R.; Hanania, Elie G.; Eisfeld, Timothy M.; Palsson, Bernhard O., Optoinjection methods.
  333. Weiss Shimon ; Bruchez ; Jr. Marcel ; Alivisatos Paul, Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such p.
  334. Shimon Weiss ; Marcel Bruchez, Jr. ; Paul Alivisatos, Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes.
  335. Leif Robert C. (Coral Gables FL), Orifice inside optical element.
  336. Remington,S. James; Hanson,George T., Oxidation reduction sensitive green fluorescent protein variants.
  337. Salzman Gary C. (Los Alamos NM) Skogen Hagenson Mary J. (Los Alamos NM), Parabolic cell analyzer.
  338. van den Engh Gerrit J. (Livermore CA) Stokdijk Willem (Livermore CA), Parallel pulse processing and data acquisition for high speed, low error flow cytometry.
  339. Kosaka Tokihiro (Kakogawa JPX), Particle analyzer.
  340. Kosaka Tokihiro (Kakogawa JPX), Particle analyzer.
  341. Kubota Fumio,JPX ; Kusuzawa Hideo,JPX, Particle analyzer.
  342. Fukuda Masakazu,JPX ; Nakamoto Hiroyuki,JPX ; Oka Masaaki,JPX, Particle analyzer classifying particles of various biological types using a correlation of measurements.
  343. Hollinger John D. (Miami FL) Pedroso Raul I. (Miami FL), Particle analyzing and sorting apparatus.
  344. Proni Oscar (Hollywood FL) James Bobby D. (Hialeah FL), Particle analyzing apparatus and method of moving particles in suspension through such apparatus.
  345. Kusuzawa Hideo (Kobe JPX), Particle analyzing apparatus using a coherence lowering device.
  346. Ludlow Ian K. (Welwyn Garden City GB2) Kaye Paul H. (Kimpton GB2), Particle asymmetry analyzer having sphericity detectors.
  347. Haynes ; John L. ; Shoor ; Bernard A., Particle density measuring system.
  348. Morgan Gary L. (Elkridge MD) Harrison Charles F. (Adelphi MD), Particle detecting instrument with sapphire detecting cell defining a rectangular flow path.
  349. Gerard de Grooth Bernard (Oldenzaal NLX) Greve Jan (Oldenzaal NLX) Terstappen Leonardus W. M. M. (Enschede NLX), Particle discriminator and method.
  350. Seiya Shinabe JP; Kunio Ueno JP, Particle measurement apparatus and method.
  351. Kubota Fumio,JPX, Particle measuring apparatus.
  352. Van den Engh Gerrit J., Particle separating apparatus and method.
  353. Van den Engh Gerrit J., Particle separating apparatus and method.
  354. Erdmann Joachim C. (Seattle WA) Gellert Robert I. (Redmond WA) Skaugset Richard L. (Kennewick WA), Particle size analyzer.
  355. Hoffman, Michael A., Particle sorter with neutralized collection wells and method of using same.
  356. Spinelli, Luis A.; Caprara, Andrea; Wang, Gary Y.; Austin, R. Russel, Passively modelocked harmonic-generating laser.
  357. Frommer Joseph C. (1525 Teakwood Ave. Cincinnati OH 45224), Photo-electric particle sensing system.
  358. Thompson Roger A. (Littleton CO), Photomultiplier tube assembly.
  359. Bergmann Ernest E. (Fountain Hill PA), Polarization independent beam splitter.
  360. Ludlow Ian K. (Welwyn Garden City GB2) Kaye Paul H. (Kimpton GB2), Portable particle analysers having plural detectors.
  361. Schindler Melvin S. (Okemos MI) Holland John F. (Lansing MI), Positive selection sorting of cells.
  362. Rodrigues Claro Jorge Antonio,BRX, Potentiometer-controlled fluid ejection device.
  363. Kwoh Deborah Y. (Carlsbad CA) Gingeras Thomas R. (Encinitas CA), Prenatal sex determination of bovine cells using male-specific oligonucleotides.
  364. Bryant Bernard J. (Davis CA), Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspr.
  365. Bawendi, Moungi; Stott, Nathan E., Preparation of nanocrystallites.
  366. Garcia, Firmin; Abergel, Aline, Pressible receptacle for a fluid sample.
  367. Carroll David C. ; Brown Dennis, Pressure vessel access port.
  368. Chupp, Vernon L., Prismatic beam expander for light beam shaping in a flow cytometry apparatus.
  369. Frasch Alberto C. (Buenos Aires ARX) Ugalde Rodolfo A. (Buenos Aires ARX), Procedure for the sex determination of embryos in mammals especially applied to bovine embryos.
  370. Tomioka Atuo (Akashishi JPX) Nakagawa Masayuki (Hyogoken JPX) Maeda Tadashi (Kobe JPX), Process and apparatus for analyzing cells.
  371. Chandler John E. (Baton Rouge LA), Process and apparatus for sorting spermatozoa.
  372. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
  373. Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
  374. Shrimpton Wallace (1015 Grandview Dr. South San Francisco CA 94080), Process for cell separation.
  375. Ranoux Claude (2 Beverly Rd. Arlington MA 02174), Process for intra-uterine fertilization in mammals and device for implementation thereof.
  376. Lawson Rommon L. (7905 Bangor Ave. Lubbock TX 79424), Process for magnetic separation and collection of viable female and male spermatozoa.
  377. Lawson Rommom L. (P.O. Box 2111 Lubbock TX 79408), Process for separation and collection of viable female and male spermatozoa.
  378. Hammerling Ulrich (New York NY) Koo Gloria C. (Woodbridge NJ) Tada Nobuhiko (New York NY), Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen.
  379. Cheng Winston Teng-Kuei,TWX ; Chen Chuan-Mu,TWX ; Hu Che-Lin,TWX ; Wang Chih-Hua,TWX ; Choo Kong-Bung,TWX, Process for sexing cow embryos.
  380. Lang ; John L., Process for the alteration of the sex-ratio of mammals.
  381. Colas Guy (Tours FR), Process for the preservation of ram semen by freezing.
  382. Wiltbank Milo (Madison WI) Pursley James R. (Sun Prairie WI), Process for the synchronization of ovulation.
  383. Buys Bruno (Lille FRX) Sozanski Jean-Pierre (Thumeries FRX) Mordon Serge (Villeneuve d\Asco FRX) Brunetaud Jean-Marc (La Madeleine FRX) Moschetto Yves (Haubourdin FRX), Process for treatment by irradiating an area of a body, and treatment apparatus usable in dermatology for the treatment.
  384. Bangham James Andrew,GBX, Pulse analysis using ordinal value filtering.
  385. Bangham James Andrew,GB2, Pulse analyzing method and apparatus.
  386. Leary James F. (Rochester NY) Todd Paul (State College PA), Pulse edge measurement for determining particle dimensional characteristics.
  387. Haugland Richard P. ; Szalecki Wojciech, Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability.
  388. Farrell Gregory A. ; Behringer Bruce E. ; Nelson John W. ; Raneri Joseph, Reagent pump assembly.
  389. Mary Buhr CA; Liwei He CA, Reduction of sperm sensitivity to chilling.
  390. Gontin Richard, Reference laser beam sampling apparatus.
  391. Gourley Paul L., Resonant-cavity apparatus for cytometry or particle analysis.
  392. Van den Engh Ger, Sample introduction apparatus for a flow cytometer.
  393. Van den Engh Ger (Seattle WA), Sample introduction system for a flow cytometer.
  394. Miyazaki Takeshi (Ebina JPX) Nishimura Matsuomi (Ohmiya JPX) Yagi Takayuki (Machida JPX) Tanaka Kazumi (Yokohama JPX) Ohnishi Toshikazu (Machida JPX) Sakuranaga Masanori (Atsugi JPX) Yoneyama Yoshito, Sample measuring device and sample measuring system.
  395. Yoneyama Yoshito (Kawasaki JPX) Toge Yoshiyuki (Yokohama JPX) Yuguchi Naoki (Yokohama JPX), Sample supply device and sample inspection apparatus using the device.
  396. Chupp Vernon (Los Altos CA), Scatter/fluorescene beam splitter in a flow cytometry apparatus.
  397. Hogg Walter R. (Miami Lakes FL), Semi-automatic adjusting delay for an electronic particle separator.
  398. Weiss Shimon ; Bruchez Marcel ; Alivisatos Paul, Semiconductor nanocrystal probes for biological applications and process for making and using such probes.
  399. Lombardo Igino (Sharon MA) Dussault Richard A. (Attleboro MA), Servo system to control the spatial position of droplet formation of a fluid jet in a cell sorting apparatus.
  400. Schinkel Otto (Bovenden DEX) Hervath Josef (Gttingen DEX), Set of instruments for the uterinal embryo transfer and intra-uterine insemination.
  401. Spaulding Glenn F. (Phoenix AZ), Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex.
  402. Spaulding Glenn F. (Chandler AZ), Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex.
  403. Spaulding Glenn F. (Chandler AZ), Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex.
  404. Kudo Toshiyuki (Ibaraki JPX) Itagaki Yoshiaki (Ibaraki JPX) Sato Seiji (Ibaraki JPX) Sutou Shizuyo (Ibaraki JPX) Nakamura Toyoo (Ibaraki JPX), Sexing method of bovine embyros.
  405. Ohki Hiroshi (Tsuchiura JPX) Miyake Ryo (Ibaraki JPX) Yamazaki Isao (Ibaraki JPX) Takahata Fujiya (Katsuta JPX), Sheath flow type flow-cell device.
  406. Seidel George ; Herickhoff Lisa ; Schenk John, Sheath fluids and collection systems for sex-specific cytometer sorting of sperm.
  407. North ; Jr. Howard L. (Los Gatos CA), Sheathed particle flow controlled by differential pressure.
  408. Mullens Patrick L. ; Emmel Gregg, Shipping container for storing materials at cryogenic temperatures.
  409. Altendorf Eric H. ; Yager Paul, Silicon microchannel optical flow cytometer.
  410. Fulwyler Mack J. (Los Alamos NM), Single drop separator.
  411. Fulwyler Mack J. (Los Alamos NM), Single drop separator.
  412. Husher Frederick K. ; Schneider Gerard ; Ramirez Lazaro, Solid state RF oscillator-detector for flow cytometer.
  413. Colon, Christopher, Sort stream stabilizer for flow cytometer.
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  2. Moreno, Juan; BeVier, Gregg; Dobrinsky, John, Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells.
  3. Moreno, Juan; Bevier, Gregg; Dobrinsky, John, Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells.
  4. van der Steen, Hein; BeVier, Gregg, Methods for use of sex sorted semen to improve genetic management in swine.
  5. van der Steen, Hein; Bevier, Gregg, Methods for use of sex sorted semen to improve genetic management in swine.
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