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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | UP-0092313 (2005-03-29) |
등록번호 | US-7838210 (2011-01-22) |
발명자 / 주소 |
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출원인 / 주소 |
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인용정보 | 피인용 횟수 : 6 인용 특허 : 469 |
Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort c
Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.
What is claimed is: 1. A method of making a gender enriched sperm cell suspension comprising mixing a sperm cell suspension with a composition which inhibits motility to form a chemically immotile sperm cell suspension, staining said chemically immotile sperm cell suspension with a DNA-selective dy
What is claimed is: 1. A method of making a gender enriched sperm cell suspension comprising mixing a sperm cell suspension with a composition which inhibits motility to form a chemically immotile sperm cell suspension, staining said chemically immotile sperm cell suspension with a DNA-selective dye and sorting the stained chemically immotile sperm cell suspension to form a viable, gender enriched insemination population of X chromosome bearing or Y chromosome bearing sperm cells having a DNA-selected dye associated with their DNA. 2. The method of claim 1, wherein the dye is selected from the group consisting of Hoechst 33342, Hoechst 33258, SYBR-14, and bisbenzimide-BODIPY® conjugate 6-{[3((2Z)-2-{[1-(difluoroboryl)-3,5-dimethyl-1H-pyrrol-2-yl] methylene}-2H-pyrrol-5-yl)propanoyl]amino}-N- [3-(methyl{3[({4- [6-(4-methylpiperazin-1-yl)-1H, 3′H-2,5′-bibenzimidazol-2′-yl]phenoxy}acetyl) amino]propyl} amino)propyl]hexanamide. 3. The method of claim 1, wherein the chemically immotile sperm cell suspension is rendered chemically immotile during the sorting process by a composition which inhibits motility comprising a source of carbonate. 4. The method of claim 3, wherein said chemically immotile sperm cell suspension is rendered chemically immotile during the sorting process by a composition which inhibits motility comprising NaHCO3, KHCO3, and C6H8O7·H2O. 5. The method of claim 4, wherein said chemically immotile sperm cell suspension is rendered chemically immotile during the sorting process by a composition which inhibits motility comprising 0.097 moles/L of NaHCO3, 0.173 moles/L of KHCO3, 0.090 moles/L C6H8O7·H2O in water. 6. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is at least 1.25×108 spermatozoa per ml. 7. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is at least 1.5×108 spermatozoa per ml. 8. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is at least 1.75×108 spermatozoa per ml. 9. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is less than about 9.0×105 spermatozoa per ml. 10. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is less than about 7×105 spermatozoa per ml. 11. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is less than about 5×105 spermatozoa per ml. 12. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is less than about 2×105 spermatozoa per ml. 13. The method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells sperm in the suspension is less than about 1×105 spermatozoa per ml. 14. The method of claim 1, wherein said viable spermatozoa suspension comprises viable frozen spermatozoa sperm. 15. The, method of claim 1, wherein a concentration of said viable, gender enriched insemination population of X chromosome bearing sperm cells or Y chromosome bearing sperm cells is sperm in the suspension being less than about 1×106 or at least about 1×108 sperm per ml. 16. The method of claim 1, wherein the chemically immotile sperm cell suspension is rendered chemically immotile during the sorting process in a suspension comprising potassium and sodium, wherein the molar ratio of potassium to sodium is greater than 1:1, respectively. 17. The method of claim 16, wherein the molar ratio of potassium to sodium is greater than 1.25:1, respectively. 18. The method of claim 16, wherein the molar ratio of potassium to sodium is greater than 1.5:1, respectively. 19. The method of claim 16, wherein the molar ratio of potassium to sodium is greater than 1.75:1, respectively. 20. The method of claim 16, wherein the molar ratio of potassium to sodium is greater than 2:1, respectively.
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