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The invention is directed to a capillary tube for electrophoresis that has a positively charged coating on the capillary inner surface that prevents positively charged analytes from adsorbing to the inner capillary surface. The capillary tube has an inner surface that is coated with a first polymer

The invention is directed to a capillary tube for electrophoresis that has a positively charged coating on the capillary inner surface that prevents positively charged analytes from adsorbing to the inner capillary surface. The capillary tube has an inner surface that is coated with a first polymer layer having a plurality of polymer groups comprising polyethylene imine, designated herein as (CH2CH2NH)x. The inner surface of the capillary typically has a second polymer layer covalently bonded to the first polymer layer. The invention includes a capillary tube where two or more than two polymer groups are covalently bonded to each other by a cross-linker. Also provided are an electrophoresis system the uses the coated capillary tubes, a method of performing electrophoresis that utilizes the coated capillary tubes, and a process for preparing the coated capillary tubes.

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What is claimed is: 1. A coated surface, the coating preventing a positively charged analyte from adsorbing to the surface, comprising a first polymer layer, the first polymer layer comprising a plurality of polymer groups comprising (CH2CH2NH)x attached by a first linker group to a first anchor gr

What is claimed is: 1. A coated surface, the coating preventing a positively charged analyte from adsorbing to the surface, comprising a first polymer layer, the first polymer layer comprising a plurality of polymer groups comprising (CH2CH2NH)x attached by a first linker group to a first anchor group, the first anchor group being covalently bonded to the surface, the surface further comprising a second polymer layer covalently bonded to the first polymer layer, the second polymer layer comprising a plurality of polymer groups comprising (CH2CH2NH)x attached by a second linker group to a second anchor group, where x is an integer independently selected for individual polymer groups within the first or second layer, and the second anchor group being covalently bonded to a first anchor group of a polymer group from the first polymer layer. 2. The surface of claim 1, wherein the first and second linker groups are an alkyl group having between 1 carbon atom and 6 carbon atoms. 3. The surface of claim 2, wherein the alkyl group has 3 carbon atoms. 4. The surface of claim 1, wherein x is between 7 and 15 for each polymer group. 5. The surface of claim 1, wherein substantially all of the polymer groups comprise linear chains of (CH2CH2NH)x. 6. The surface of claim 1, wherein at least some individual polymer groups comprise branched chains where one or more than one nitrogen is a secondary or tertiary amine covalently bound to one or more (CH2CH2NH)x group. 7. The surface of claim 1, wherein one or more (CH2CH2NH)x group from two or more than two different polymer groups are covalently bonded to each other by a cross-linker. 8. The surface of claim 7, wherein the cross-linker is selected from the group consisting of: 1,4-butanediol diglicidylether, ethylene glycol diglycidyl ether, triglycidyl glycerol, and diglycidyl glycerol. 9. The surface of claim 1, wherein the first polymer layer covalently bonded to the surface and the second polymer layer covalently bonded to the first polymer layer has the following structure: wherein CS is the surface, N is nitrogen, O is oxygen, Si is silicon, x and y are integers between 7 and 15 independently selected for individual polymer groups, R1 is selected from the group consisting of hydrogen, alkyl groups and halogens, R2 and R3 are independently selected from the group consisting of hydrogen, alkyl groups, and (CH2CH2NH)z where z is an integer between 1 and 15, n and m are integers between 1 and 8 that are independently selected for individual polymer groups, CR is a cross-linker that covalently bonds to one or more (CH2CH2NH)x polymer group to one or more (CH2CH2NH)y polymer group, and H is hydrogen, with the proviso that H is not present when the N to which it is attached is crosslinked to a CR group. 10. The surface of any one of claims 1 to 9 wherein the surface comprises silica. 11. The coated surface according to claim 1, said coated surface made by a process for the preparation of a surface comprising: a) treating the surface with a base; b) treating the surface with a solution containing between 2% and 30% trimethoxysilylpropyl (polyethyleneimine) to form a polymer group; c) rinsing the surface; and d) treating the surface with a cross-linker to cross-link at least one polymer group. 12. The coated surface according to claim 1, wherein said coated surface is within a silica capillary tube having a coating, said silica capillary tube having a coating made by a process for the preparation of a silica capillary tube with a coating comprising the steps of: a) treating the inner surface of the silica capillary tube with a base; b) treating the inner surface of the capillary tube with a solution containing between 2% and 30% trimethoxysilylpropyl (polyethyleneimine) to form a polymer group; c) rinsing the inner surface of the capillary tube; and d) treating the inner surface with a cross-linker to cross-link at least one polymer group. 13. A coated surface wherein at least a portion of the surface is coated with a first polymer layer, the first polymer layer comprising a plurality of polymer groups, each polymer group comprising a polymer of the formula (CH2CH2NH)x, which is attached by a linker group of the formula to an anchor group, the anchor group being covalently bonded to the surface, where two or more than two polymer groups are covalently bonded to each other by a cross-linker, and where X is an integer greater than 5, R3 is selected from the group consisting of hydrogen, alkyl groups, and (CH2CH2NH)z where z is an integer between 1 and 15, and n is an integer between 1 and 8. 14. The surface of claim 13, wherein n is an integer between 1 and 6 carbon atoms. 15. The surface of claim 14, wherein n is 3. 16. A surface coated with a first polymer layer having the following structure: wherein CS is the surface, N is nitrogen, O is oxygen, Si is silicon, R1, R2R3, and R4 are independently selected from the group consisting of hydrogen, alkyl groups and halogens, R5 and R6 are independently selected from the group consisting of hydrogen, alkyl groups, and one or more than one (CH2CH2NH) moiety, where x and y are integers between 7 and 15, n and m are integers independently selected for individual polymer groups that is between 1 and 8, CR is a cross-linker that covalently bonds one or more (CH2CH2NH)x polymer group to one or more (CH2CH2NH)y polymer group, and H is hydrogen, with the proviso that H is not present when the N to which it is attached is cross-linked to a CR group. 17. The surface of claim 16, comprising a second polymer layer covalently bound to the first polymer layer. 18. The surface of claim 17, comprising a third polymer layer covalently bonded to the second polymer layer. 19. The surface of claim 16, wherein the cross-linker is selected from the group consisting of: 1,4-butanediol diglicidylether, ethylene glycol diglycidyl ether, triglycidyl glycerol, and diglycidyl glycerol. 20. A method of performing electrophoresis, the method comprising the steps of: providing a capillary electrophoresis apparatus; selecting a capillary tube having an inner surface and an outer surface, at least a portion of the inner surface coated with a first polymer layer, the first polymer layer comprising a plurality of polymer groups comprising (CH2CH2NH)x attached by a first linker group to a first anchor group, the first anchor group being covalently bonded to the inner surface of the capillary, the inner surface of the capillary further comprising a second polymer layer covalently bonded to the first polymer layer, the second polymer layer comprising a plurality of polymer groups comprising (CH2CH2NH)x attached by a second linker group to a second anchor group where x is an integer independently selected for individual polymer groups within the first or second layer, and the second anchor group being covalently bonded to a first anchor group of a polymer group from the first polymer layer; providing a sample comprising one or more than one compound; loading the sample into the capillary tube; performing electrophoresis on the sample; and detecting one or more than one compound from the sample.