Microorganisms for the production of 1,4-butanediol
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-007/18
C12N-001/21
출원번호
UP-0556550
(2009-09-09)
등록번호
US-7858350
(2011-02-24)
발명자
/ 주소
Burk, Mark J.
Burgard, Anthony P.
Osterhout, Robin E.
Sun, Jun
출원인 / 주소
Genomatica, Inc.
대리인 / 주소
McDermott Will & Emery LLP
인용정보
피인용 횟수 :
41인용 특허 :
88
초록▼
The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbi
The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
대표청구항▼
What is claimed is: 1. A host microbial organism transformed with nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, said microbial organism comprising exogenous nucleic acids encoding BDO pathway enzymes expressed in a sufficient amount to produce BDO, said BDO pathway enzymes compr
What is claimed is: 1. A host microbial organism transformed with nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, said microbial organism comprising exogenous nucleic acids encoding BDO pathway enzymes expressed in a sufficient amount to produce BDO, said BDO pathway enzymes comprising: a) 3-hydroxybutyryl-CoA dehydrogenase, said enzyme classified as EC 1.1.1.a and converting acetoacetyl-coenzyme A (CoA) to 3-hydroxybutyryl-CoA; b) 3-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting 3-hydroxybutyryl-CoA to crotonoyl-CoA; c) vinylacetyl-CoA A-isomerase, said enzyme classified as EC 5.3.3.3 and converting crotonoyl-CoA to vinylacetyl-CoA; d) 4-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting vinylacetyl-CoA to 4-hydroxybutyryl-CoA; and e) 4-hydroxybutyryl-CoA reductase (alcohol forming), said enzyme classified as EC 1.1.1.c and converting 4-hydroxybutyryl-CoA to 1,4-butanediol; or 4-hydroxybutyryl-CoA reductase, said enzyme classified as EC 1.2.1.b and converting 4-hydroxybutyryl-CoA to 4-hydroxybutanal, and 1,4-butanediol dehydrogenase, said enzyme classified as EC 1.1.1.a and converting 4-hydroxybutanal to 1,4-butanediol. 2. The host microbial organism of claim 1, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase (alcohol forming). 3. The host microbial organism of claim 1, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase and 1,4-butanediol dehydrogenase. 4. The host microbial organism of claim 1, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase (alcohol forming), 4-hydroxybutyryl-CoA reductase, and 1,4-butanediol dehydrogenase. 5. The host microbial organism of claim 1, wherein at least one exogenous nucleic acid is a heterologous nucleic acid. 6. The host microbial organism of claim 1, wherein said host microbial organism is in a substantially anaerobic culture medium. 7. A method for producing BDO, comprising culturing the host microbial organism of claim 1 under conditions and for a sufficient period of time to produce BDO. 8. The method of claim 7, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase (alcohol forming). 9. The method of claim 7, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase and 1,4-butanediol dehydrogenase. 10. The method of claim 7, wherein said BDO pathway comprises 4-hydroxybutyryl-CoA reductase (alcohol forming), 4-hydroxybutyryl-CoA reductase, and 1,4-butanediol dehydrogenase. 11. The method of claim 7, wherein said non naturally occurring host microbial organism is in a substantially anaerobic culture medium. 12. The method of claim 7, wherein at least one exogenous nucleic acid is a heterologous nucleic acid.
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