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DNA polymerases having improved labeled nucleotide incorporation properties

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07H-021/02
출원번호 US-0299124 (2005-12-09)
등록번호 US7897738 (2011-02-15)
발명자 / 주소
  • Brandis, John
  • Bloom, Curtis
  • Richards, John H.
출원인 / 주소
  • Applied Biosystems, LLC
대리인 / 주소
    Scott R., Bortner
인용정보 피인용 횟수 : 29  인용 특허 : 52

초록

The present teachings relate to DNA polymerases that have an F667Y mutation as defined with respect to Taq DNA polymerase and exhibit reduced discrimination against labeled nucleotides into polynucleotides.The teachings also include kits comprising the subject DNA polymerases and numerous methods of

대표청구항

What is claimed is: 1. A composition comprising a first DNA polymerase and a second DNA polymerase, wherein the first and the second DNA polymerase have an F667Y mutation as defined with respect to Taq DNA polymerase, and wherein the first DNA polymerase and the second DNA polymerase have primary am

이 특허에 인용된 특허 (52)

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  1. Rothberg, Jonathan M.; Kabiri, Ali; Sickler, Jason W.; Gyarfas, Brett J.; Lackey, Jeremy; Schmid, Gerard, Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens.
  2. Davis, Randall; Chen, Roger; Bibillo, Arkadiusz; Deierling, Kevin, Chip set-up and high-accuracy nucleic acid sequencing.
  3. Ju, Jingyue, DNA sequencing by nanopore using modified nucleotides.
  4. Rothberg, Jonathan M.; Fife, Keith G.; Boisvert, David, Integrated device for temporal binning of received photons.
  5. Rothberg, Jonathan M.; Kabiri, Ali; Sickler, Jason W.; Gyarfas, Brett J.; Lackey, Jeremy; Schmid, Gerard; Cipriany, Benjamin; Jewell, Jack; West, Lawrence; Ferrigno, Michael; Glenn, Paul E.; Bellofiore, Anthony, Integrated device with external light source for probing detecting and analyzing molecules.
  6. Rothberg, Jonathan M.; Kabiri, Ali; Sickler, Jason W.; Gyarfas, Brett J.; Lackey, Jeremy; Schmid, Gerard; West, Lawrence C.; Fife, Keith G.; Cipriany, Benjamin; Ghasemi, Farshid, Integrated device with external light source for probing detecting and analyzing molecules.
  7. Chen, Roger J. A.; Davis, Randy, Methods for forming a nanopore in a lipid bilayer.
  8. Davis, Randall W.; Liu, Edward Shian; Harada, Eric Takeshi; Aguirre, Anne; Trans, Andrew; Pollard, James; Cech, Cynthia, Methods for forming lipid bilayers on biochips.
  9. Chen, Roger J. A.; Fullagar, David J., Nanopore arrays.
  10. Chen, Roger J. A.; Fullagar, David J., Nanopore arrays.
  11. Davis, Randall W.; Chen, Roger J. A., Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps.
  12. Davis, Randall W.; Chen, Roger J. A., Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps.
  13. Davis, Randall W.; Chen, Roger J. A., Nanopore-based single molecule characterization.
  14. Chen, Roger J. A., Non-faradaic, capacitively coupled measurement in a nanopore cell array.
  15. Davis, Randall; Chen, Roger; Bibillo, Arkadiusz; Korenblum, Daniel, Nucleic acid sequencing using tags.
  16. Rothberg, Jonathan M.; Kabiri, Ali; Sickler, Jason W.; Gyarfas, Brett J.; Lackey, Jeremy; Schmid, Gerard; Cipriany, Benjamin; Jewell, Jack; West, Lawrence; Ferrigno, Michael; Glenn, Paul E.; Cohen, Adam E.; Bellofiore, Anthony, Optical system and assay chip for probing, detecting and analyzing molecules.
  17. Rothberg, Jonathan M.; Kabiri, Ali; Sickler, Jason W.; Gyarfas, Brett J.; Lackey, Jeremy; Schmid, Gerard; Glenn, Paul E.; West, Lawrence C.; Cipriany, Benjamin; Fife, Keith G., Optical system and assay chip for probing, detecting and analyzing molecules.
  18. Davis, Randall W.; Liu, Edward Shian; Harada, Eric Takeshi; Aguirre, Anne; Trans, Andrew; Pollard, James; Cech, Cynthia; Tian, Hui; Yuan, Robert; Foster, John; Chen, Roger, Process for biosensor well formation.
  19. Deierling, Kevin; Chen, Roger J. A.; Fullagar, David J., Sensor circuit for controlling, detecting, and measuring a molecular complex.
  20. Chen, Roger, System for communicating information from an array of sensors.
  21. Chen, Roger, System for detecting electrical properties of a molecular complex.
  22. Chen, Roger J. A., System for detecting electrical properties of a molecular complex.
  23. Chen, Roger J. A.; Davis, Randy, Systems and methods for characterizing a molecule.
  24. Chen, Roger J. A.; Davis, Randy, Systems and methods for characterizing a molecule.
  25. Chen, Roger; Davis, Randy, Systems and methods for forming a nanopore in a lipid bilayer.
  26. Chen, Roger, Temperature regulation of measurement arrays.
  27. Chen, Roger J. A., Temperature regulation of measurement arrays.
  28. Bolchakova, Elena; Rozzelle, James, Thermus brockianus nucleic acid polymerases.
  29. Bolchakova, Elena; Rozzelle, James, Thermus scotoductus nucleic acid polymerases.
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