초록

A method of analyzing cellular samples that include a chemically crosslinked analyte is provided. The analysis typically involves the use of mass spectrometry.

대표청구항 ▼

What is claimed is: 1. A method of analyzing analytes, the method comprising:providing a cellular sample comprising chemically crosslinked analytes, wherein the sample is one or more formalin-fixed, paraffin-embedded tissue sections, and wherein the analytes are proteins or peptides or combinations

What is claimed is: 1. A method of analyzing analytes, the method comprising:providing a cellular sample comprising chemically crosslinked analytes, wherein the sample is one or more formalin-fixed, paraffin-embedded tissue sections, and wherein the analytes are proteins or peptides or combinations thereof;separating the chemically crosslinked analytes from the formalin-fixed, paraffin-embedded tissue sections by heat;reversing at least a portion of the chemical crosslinks in the crosslinked analytes and forming decrosslinked analytes, wherein said reversing at least a portion of the chemical crosslinks comprises cleaving at least a portion of the chemical crosslinks, with the proviso that substantially no naturally occurring bonds in the analytesare cleaved during said reversing at least a portion of the chemical crosslinks; andanalyzing the analytes by analyzing the decrosslinked analytes using mass spectrometry.