IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0815171
(2010-06-14)
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등록번호 |
US-8101064
(2012-01-24)
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발명자
/ 주소 |
- Forrow, Nigel John
- Zhang, Xiang Cheng
- McTigue, Catherine Ann
|
출원인 / 주소 |
|
대리인 / 주소 |
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인용정보 |
피인용 횟수 :
0 인용 특허 :
10 |
초록
▼
A biosensor for determining the concentration of an analyte in a biological sample. The biosensor comprises a support, a reference electrode or a counter electrode or both disposed on the support, a working electrode disposed on the support, the working electrode spaced apart from the other electrod
A biosensor for determining the concentration of an analyte in a biological sample. The biosensor comprises a support, a reference electrode or a counter electrode or both disposed on the support, a working electrode disposed on the support, the working electrode spaced apart from the other electrode or electrodes on the support, a covering layer defining a sample chamber over the electrodes, an aperture in the covering layer for receiving a sample, and at least one layer of mesh in the sample chamber between the covering layer and the electrodes. The at least one layer of mesh has coated thereon a silicone surfactant. Certain silicone surfactants are as effective as fluorinated surfactants with respect to performance of biosensors. These surfactants, when coated onto the mesh layer of the biosensor, are effective in facilitating the transport of aqueous test samples, such as blood, in the sample chamber.
대표청구항
▼
1. A method for determining the concentration of an analyte in a sample, the method comprising: applying the sample to a biosensor, the biosensor comprising: a substrate;a working electrode;a counter electrode;a cover layer defining an enclosed space over the electrodes; and at least one mesh layer
1. A method for determining the concentration of an analyte in a sample, the method comprising: applying the sample to a biosensor, the biosensor comprising: a substrate;a working electrode;a counter electrode;a cover layer defining an enclosed space over the electrodes; and at least one mesh layer disposed in the enclosed space between the cover layer and the electrodes, the at least one mesh layer coated with at least one silicone surfactant, wherein the at least one silicone surfactant comprises a mixture of silicone surfactants;applying a voltage to the working electrode;measuring a current at the working electrode; andcorrelating the current measured with the concentration of the analyte in the sample. 2. The method according to claim 1, wherein the mixture of silicone surfactants comprises a high molecular weight silicone surfactant and a low molecular weight silicone surfactant. 3. The method of claim 2, wherein the high molecular weight silicone surfactant and the low molecular weight silicone surfactant have the formula: wherein x ranges from 8 to 9, inclusive, y ranges from 3 to 4, inclusive, z ranges from 11 to 13, inclusive, and R being hydrogen for the high molecular weight silicone surfactant; andx is 0, y is 1, z ranges from 3 to 15, inclusive, and R is hydrogen, methyl, or acetate for the low molecular weight silicone surfactant. 4. The method of claim 2, wherein the weight fraction of the high molecular weight silicone surfactant in the mixture ranges from 1% to 99%, inclusive. 5. The method of claim 2, wherein the weight fraction of the low molecular weight silicone surfactant in the mixture ranges from 1% to 99% inclusive. 6. The method of claim 1, wherein the working electrode comprises a working ink, the working ink comprising an analyte responsive enzyme and a mediator. 7. The method of claim 6, wherein the analyte responsive enzyme is selected from the group consisting of glucose oxidase, glucose dehydrogenase and 3-hydroxybutyrate dehydrogenase. 8. The method of claim 1, wherein the biosensor comprises a single layer of mesh. 9. The method of claim 1, wherein the biosensor comprises two layers of mesh. 10. The method of claim 1, wherein the at least one mesh layer comprises polyester or polyamide. 11. The method of claim 1, wherein the at least one mesh layer is less than 150 microns in thickness. 12. The method of claim 1, wherein the at least one mesh layer comprises fibers having a diameter from about 70 to about 100 microns. 13. A method for determining the concentration of an analyte in a sample, the method comprising: applying the sample to a biosensor, the biosensor comprising: a substrate;a working electrode;a counter electrode;a cover layer defining an enclosed space over the electrodes; andat least one mesh layer disposed in the enclosed space between the cover layer and the electrodes, the at least one mesh layer coated with at least one silicone surfactant, wherein the at least one silicone surfactant comprises a trisiloxane surfactant;applying a voltage to the working electrode;measuring a current at the working electrode; andcorrelating the current measured with the concentration of the analyte in the sample. 14. The method of claim 13, wherein the working electrode comprises a working ink, the working ink comprising an analyte responsive enzyme and a mediator. 15. The method of claim 14, wherein the analyte responsive enzyme is selected from the group consisting of glucose oxidase, glucose dehydrogenase and 3-hydroxybutyrate dehydrogenase. 16. The method of claim 13, wherein the biosensor comprises two layers of mesh. 17. The method of claim 13, wherein the at least one mesh layer comprises polyester or polyamide. 18. The method of claim 13, wherein the at least one mesh layer is less than 150 microns in thickness. 19. The method of claim 13, wherein the at least one mesh layer comprises fibers having a diameter from about 70 to about 100 microns. 20. A method for determining the concentration of an analyte in a sample, the method comprising: applying the sample to a biosensor, the biosensor comprising: a substrate;a working electrode;a counter electrode;a cover layer defining an enclosed space over the electrodes; andat least one mesh layer disposed in the enclosed space between the cover layer and the electrodes, the at least one mesh layer coated with at least one silicone surfactant, wherein the at least one silicone surfactant comprises a cyclosiloxane surfactant;applying a voltage to the working electrode;measuring a current at the working electrode; andcorrelating the current measured with the concentration of the analyte in the sample. 21. The method of claim 20, wherein the working electrode comprises a working ink, the working ink comprising an analyte responsive enzyme and a mediator. 22. The method of claim 21, wherein the analyte responsive enzyme is selected from the group consisting of glucose oxidase, glucose dehydrogenase and 3-hydroxybutyrate dehydrogenase. 23. The method of claim 20, wherein the biosensor comprises two layers of mesh. 24. The method of claim 20, wherein the at least one mesh layer comprises polyester or polyamide. 25. The method of claim 20, wherein the at least one mesh layer is less than 150 microns in thickness. 26. The method of claim 20, wherein the at least one mesh layer comprises fibers having a diameter from about 70 to about 100 microns.
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