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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0027835 (2008-02-07) |
등록번호 | US-8114270 (2012-02-14) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
|
인용정보 | 피인용 횟수 : 0 인용 특허 : 447 |
A sensor designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator. The redox mediator acts as an electron transfer agent between the analyte and the electrode. In a
A sensor designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator. The redox mediator acts as an electron transfer agent between the analyte and the electrode. In addition, a second electron transfer agent, such as an enzyme, can be added to facilitate the electrooxidation or electroreduction of the analyte. The redox mediator is typically a redox compound bound to a polymer. The preferred redox mediators are air-oxidizable. The amount of analyte can be determined by coulometry. One particular coulometric technique includes the measurement of the current between the working electrode and a counter or reference electrode at two or more times. The charge passed by this current to or from the analyte is correlated with the amount of analyte in the sample. Other electrochemical detection methods, such as amperometric, voltammetric, and potentiometric techniques, can also be used. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is provided as a second electron transfer agent.
1. A method of determining the concentration of an analyte in a biological fluid, the method comprising: providing an in vitro biosensor comprising: i) at least two plates, wherein at least one of said plates forms a boundary area to house no more than 1 μl of a biological fluid;ii) at least two ele
1. A method of determining the concentration of an analyte in a biological fluid, the method comprising: providing an in vitro biosensor comprising: i) at least two plates, wherein at least one of said plates forms a boundary area to house no more than 1 μl of a biological fluid;ii) at least two electrodes, wherein one electrode is a working electrode and one electrode is a reference electrode, counter electrode or counter/reference electrode;iii) at least one reagent layer associated with said working electrode;applying a biological fluid to said boundary area by capillary action;detecting said biological fluid in said boundary area;measuring said biological fluid in said boundary area within 1 minute of said applying; andproviding by coulometry an actual concentration of said analyte in said biological fluid. 2. The method according to claim 1, wherein said reference electrode, counter electrode, or counter/reference electrode is upstream of said working electrode. 3. The method according to claim 1, wherein said reagent layer includes an enzyme-based composition. 4. The method according to claim 3, wherein said enzyme-based composition comprises glucose oxidase or glucose dehydrogenase. 5. The method according to claim 3, wherein said reagent layer includes a redox mediator selected from the group consisting of an osmium complex, ferricyanide and ferrocyanide. 6. The method according to claim 3, wherein any of said plurality of electrodes are constructed of materials selected from the group consisting of gold, platinum, ruthenium dioxide, palladium, and other non-corroding materials. 7. The method according to claim 3, further comprising an opening downstream of said reagent layer. 8. The method according to claim 1, wherein any of said plurality of electrodes are constructed of carbon or carbon-like materials. 9. The method according to claim 1, wherein said biosensor houses a volume of biological fluid from about 0.1 μL to about 1.0 μL. 10. The method according to claim 1, wherein said analyte is glucose. 11. The method according to claim 10, wherein any of said plurality of electrodes are constructed of carbon or carbon-like materials. 12. The method according to claim 1, wherein said reference electrode, counter electrode, or counter/reference electrode is separated from said working electrode by a distance of less than 200 micrometer. 13. A method of determining the concentration of an analyte in a biological fluid, the method comprising: providing an in vitro biosensor comprising: at least two plates, wherein at least one of said plates form at least one boundary area wherein a biological fluid is analyzed, wherein said boundary area receives a volume of biological fluid no greater than 1.5 μL;a plurality of electrodes, wherein said plurality of electrodes includes at least a working electrode and at least a reference electrode, counter electrode or counter-reference electrode;a tip fill region associated with said boundary area for the transport by capillary action of no greater than 1.0 μL of said biological fluid; anda reagent layer including an enzyme-based composition including glucose oxidase and ferricyanide, wherein said reagent layer is associated with said working electrodeapplying a biological fluid to said boundary area by capillary action;detecting said biological fluid in said boundary area;measuring said biological fluid in said boundary area within 1 minute of said applying; andproviding by coulometry an actual concentration of said analyte in said biological fluid. 14. The method according to claim 13, wherein said biosensor receives a volume of biological fluid of no more than 0.5 μL. 15. The method according to claim 13, wherein any of said plurality of electrodes are constructed of materials selected from the group consisting of gold, platinum, ruthenium dioxide, palladium, and other non-corroding materials. 16. The method according to claim 13, further comprising an opening downstream of said reagent layer. 17. The method according to claim 13, wherein said analyte is glucose. 18. The method according to claim 13, wherein said reference electrode, counter electrode, or counter/reference electrode is separated from said working electrode by a distance of less than 200 micrometer. 19. The method according to claim 13, wherein said reference electrode, counter electrode, or counter/reference electrode is upstream of said working electrode.
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