[특허]Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics

Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/68 C12Q-001/00
출원번호	US-0726520 (2007-03-22)
등록번호	US-8114589 (2012-02-14)
발명자 / 주소	Sosnowski, Ronald G. Butler, William F. Tu, Eugene Nerenberg, Michael I. Heller, Michael J. Edman, Carl F.
출원인 / 주소	Gamida For Life B.V.
대리인 / 주소	White, John P.
인용정보	피인용 횟수 : 0 인용 특허 : 90

초록 ▼

A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.

대표청구항 ▼

1. A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device, comprising the steps of: providing a first negatively charged nucleic acid bound to the test site, wherein said test site is generated using a technique selected from the group consisting of a microlithographic technique and a micromachining technique;providing a solution including a second negatively charged nucleic acid;applying a zwitterionic buffer having a conductance of less than 100 mS/cm to the microelectronic device; andapplying current to the test site to positively bias the test site,wherein the second negatively charged nucleic acid is transported to the positively biased test site having the first negatively charged nucleic acid,wherein the first and second negatively charged nucleic acids hybridize,wherein the zwitterionic buffer acquires a net positive charge under influence of the current, wherein the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids. 2. The method of claim 1, wherein the zwitterionic buffer comprises histidine. 3. The method of claim 1, wherein the isoelectric point of said zwitterionic buffer is about pH 7.47. 4. The method of claim 1, wherein the zwitterionic buffer is cationic. 5. The method of claim 1, wherein the zwitterionic buffer is dicationic. 6. The method of claim 1, wherein the zwitterionic buffer is polycationic.

이 특허에 인용된 특허 (90)

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Stanbro William D. (Columbia MD) Hunter ; Jr. Kenneth W. (Potomac MD) Newman Arnold L. (Kensington MD), Added array of molecular chains for interfering with electrical fields.
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Southern Edwin,GBX, Analying polynucleotide sequences.
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Begg Geoffrey Stephen,AUX ; Simpson Richard John,AUX ; Burgess Antony Wilks,AUX, Apparatus allowing sequential chemical reactions.
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Elings, Virgil B.; Nicoli, David F., Apparatus and method for homogeneous immunoassay.
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Sheldon ; III Edward L. ; Jackson Thomas R. ; Swanson Paul D. ; Scott Bradley S. ; Heller Michael J., Apparatus and methods for active biological sample preparation.
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Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Array of oligonucleotides on a solid substrate.
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Palva Airi M. (Helsinki FIX) Ranki Tuula M. (Espoo FIX) Sderlund Hans E. (Espoo FIX), Arrays of alternating nucleic acid fragments for hybridization arrays.
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Fodor Stephen P.A. ; Stryer Lubert ; Read J. Leighton ; Pirrung Michael C., Arrays of materials attached to a substrate.
상세보기
Comfort John J. (Berkshire GB2) Hayter Paul A. (Berkshire GB2) Kargathra Dinesh (Middlesex GB2) Mason Brian R. (Berkshire GB2) Turner Graham N. (Berkshire GB2) Fisher Ian R. (Middlesex GB2) Bailey Jo, Automatic test equipment.
상세보기
Hu Ho-Pin (Ann Arbor MI) Katsnelson Inessa (Ann Arbor MI) Sellinger Alan (Ann Arbor MI), Cationic charge modified microporous membrane.
상세보기
Mattingly Phillip G. (Grayslake IL) Bennett Larry G. (Grayslake IL), Chemiluminescent acridinium salts.
상세보기
Heller, Michael J.; Tu, Eugene, Control system for active programmable electronic microbiology system.
상세보기
Ranki Tuula M. (Espoo FIX) Soderlund Hans E. (Espoo FIX), Detection of microbial nucleic acids by a one-step sandwich hybridization test.
상세보기
Cheung Peter W. (Mercer Island WA) Wieler Edward B. (Seattle WA) Furlong ; Jr. Clement E. (Seattle WA), Digital biosensors and method of using same.
상세보기
Lauks Imants R. (Yardley PA) Wieck Henry J. (Brooklyn NY) Zelin Michael P. (Plainsboro NJ) Blyskal Philip (Plainsboro NJ), Disposable sensing device for real time fluid analysis.
상세보기
Stanley Christopher J. (St. Ives GB2), Electrochemical denaturation of double-stranded nucleic acid.
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상세보기
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상세보기
Hjertn Stellan (Uppsala SEX) Liao Jia-Li (Uppsala SEX), Electrophoresis in low conductivity buffers.
상세보기
Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA) Zemel Jay N. (Jenkintown PA), Fluid handling in mesoscale analytical devices.
상세보기
Heller Michael J. (Encinitas CA), Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfe.
상세보기
Smith Henry M. ; Schicker James R., Hydrogen and moisture getter and absorber for sealed devices.
상세보기
Leaback David H. (Radlett GB2), Imaging immunoassay detection system with background compensation and its use.
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Johnson Kirk W. (Indianapolis IN), Immobolization of biomolecules by enhanced electrophoretic precipitation.
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Pace Salvatore J. (Yorktown Heights NY), Integrated array of electrochemical sensors.
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Broadbent Neal E. (El Dorado Hills CA) MacGregor Duncan D. (Shingle Springs CA), Interconnection scheme for integrated circuit card with auxiliary contacts.
상세보기
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
상세보기
Wilding Peter ; Kricka Larry J., Mesoscale sample preparation device and systems for determination and processing of analytes.
상세보기
Begg Geoffrey Stephen (Heidelberg AUX) Simpson Richard John (Richmond AUX) Burgess Antony Wilks (Camberwell AUX), Method allowing sequential chemical reactions.
상세보기
Fodor Stephen P. A. (Palo Alto CA) Mazzola Laura T. (Redwood City CA), Method and apparatus for measuring binding affinity.
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Mack Daniel R. (Half Moon Bay CA) Konicek Michael G. (Cupertino CA), Method and apparatus for performing automated, multi-sequential immunoassays.
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Soane David S. (Piedmont CA) Soane Zoya M. (Piedmont CA), Method and device for moving molecules by the application of a plurality of electrical fields.
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Soane David S. ; Soane Zoya M., Method and device for moving molecules by the application of a plurality of electrical fields.
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Drmanac Radoje T. (Zvecanska 46 Beograd 11000) Crkvenjakov Radomir B. (Bulevar JNA 118 Beograd YUX 11000), Method of sequencing of genomes by hybridization of oligonucleotide probes.
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Heller Michael J. ; Tu Eugene ; Evans Glen A. ; Sosnowski Ronald G., Methods for transport in molecular biological analysis and diagnostics.
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DeBoer Charles D. (Irondequoit NY) Kamp Dennis R. (Spencerport NY) Mey William (Greece NY), Migration imaging system.
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Hafeman Dean G. (San Bruno CA) McConnell Harden M. (Stanford CA), Multiple chemically modulated capacitance determination.
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Kovacs Gregory T. A., Multiplexed active biologic array.
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Tice ; Jr. George (Wenonah NJ) Amorese Douglas A. (Hockessin DE), Neutral and positively charged dyes for electrophoresis sample loading solutions.
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Hirschfeld Tomas B. (Livermore CA), Nucleic acid assay method.
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Klevan Leonard (Washington DC) Gebeyehu Gulilat (Silver Spring MD) Rao Prasad Y. (Damascus MD), Nucleotide analogs for nucleic acid labeling and detection.
상세보기
Hollis Mark A. (Concord MA) Ehrlich Daniel J. (Lexington MA) Murphy R. Allen (Boxboro MA) Kosicki Bernard B. (Acton MA) Rathman Dennis D. (Ashland MA) Chen Chang-Lee (Sudbury MA) Mathews Richard H. (, Optical and electrical methods and apparatus for molecule detection.
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Hollis Mark A. ; Ehrlich Daniel J. ; Murphy R. Allen ; Kosicki Bernard B. ; Rathman Dennis D. ; Mathews Richard H. ; Burke Barry E. ; Eggers Mitch D. ; Hogan Michael E. ; Varma Rajender Singh, Optical and electrical methods and apparatus for molecule detection.
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Bjornson Torleif O. (Milpitas CA) Pfost R. Fred (Los Altos CA), Optical detection module for use in an automated laboratory work station.
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Konrad Michael W., Optical detection of position of oligonucleotides on large DNA molecules.
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Newman Arnold L. (Kensington MD), Optimized capacitive sensor for chemical analysis and measurement.
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Zanzucchi Peter J. (West Windsor Township NJ) Cherukuri Satyam C. (Cranbury NJ) McBride Sterling E. (Lawrence Township NJ) Judd Amrit K. (Belmont CA), Partitioned microelectronic device array.
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Evans Glen A. (Encinitas CA), Physical mapping of complex genomes.
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Mandecki Wlodek (Vernon Hills IL) Hayden Mark A. (Vernon Hills IL), Polycationic buffers and method for gel electrophoresis of nucleic acids.
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Patel Shailen S. (Santa Monica CA), Sample-scanning photometer.
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Osman Peter D. J. (West Lindfield AUX) Cornell Bruce A. (Neutral Bay AUX) Raguse Burkhard (St Ives AUX) King Lionel G. (Marsfield AUX), Sensitivity and selectivity of ion channel biosensor membranes.
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Okano Kazunori (Shiki JPX) Kambara Hideki (Hachiouji JPX), Separation of polynucleotides using supports having a plurality of electrode-containing cells.
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Durley ; III Benton A. (Antioch IL) Defreese James D. (Wildwood IL) Merkh Carl W. (Lindenhurst IL), Test card for performing assays.
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Mikkelsen Susan R. (Montreal CAX) Millan Kelly M. (LaSalle CAX) Spurmanis Aleksandrs J. (Montreal CAX), Voltammetric sequence-selective sensor for target polynucleotide sequences.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

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연합인증

Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics 원문보기

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대표청구항 ▼

연구과제 타임라인

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