The present invention relates to methods, reagents, and substrates that can be used for, for example, immobilizing biomolecules, such as nucleic acids and proteins. In an embodiment, the present invention relates to surfaces coated with a polymer according to the present invention. In an embodiment,
The present invention relates to methods, reagents, and substrates that can be used for, for example, immobilizing biomolecules, such as nucleic acids and proteins. In an embodiment, the present invention relates to surfaces coated with a polymer according to the present invention. In an embodiment, the present invention relates to methods for thermochemically and/or photochemically attaching molecules to a surface at a high density.
대표청구항▼
1. A substrate comprising a surface and a polymer, the polymer coating at least a portion of the surface; the polymer comprising: at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; andone or more pendent reactive groups configured to form covalent bond
1. A substrate comprising a surface and a polymer, the polymer coating at least a portion of the surface; the polymer comprising: at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; andone or more pendent reactive groups configured to form covalent bond with biomolecule, wherein one or more pendent reactive groups are selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof. 2. The substrate of claim 1, wherein the polymer comprises: N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;ethyleneglycol, ethyleneimine, PEG derivative of mono methacrylate, or mixtures thereof;aminimide; or mixtures thereof. 3. A substrate comprising: support surface; andpolymer coupled to the support surface; the polymer comprising: at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; andmore than one reactive group selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;wherein the polymer is configured to form covalent bonds with biomolecule. 4. A composition comprising polymer; the polymer comprising: at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; andone or more reactive groups configured to form covalent bonds with corresponding functional groups on biomolecule, wherein one or more pendent reactive groups are selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;the polymer being configured to be covalently attached to surface. 5. The composition of claim 4, wherein the polymer comprises: N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;ethyleneglycol, ethyleneimine, PEG derivative of monomethacrylate, or mixtures thereof;aminimide; ormixtures thereof 6. The composition of claim 4, wherein the polymer coating comprises a monomer comprising an uncharged polar moiety other than primary amide in an amount effective to provide, when coated on a surface of a substrate, a contact angle of about 30° to about 60° for a sessile water drop. 7. A micro array comprising: support surface;polymer covalently coupled to the support surface; the polymer comprising: at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; andone or more reactive groups selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;biomolecule covalently bound to the polymer in discrete spots. 8. The micro array of claim 7, wherein the polymer comprises: N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;ethyleneglycol, ethyleneimine, PEG derivative of monomethacrylate, or mixtures thereof;aminimide; ormixtures thereof. 9. The microarray of claim 7, wherein the polymer coating comprises a monomer comprising an uncharged polar moiety other than primary amide in an amount effective to provide a contact angle of about 30° to about 60° for a sessile water drop.
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이 특허에 인용된 특허 (31)
Ofstead,Ronald F.; Swanson,Melvin J.; Swan,Dale G., Attachment of molecules to surfaces.
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Swanson Melvin J. ; Amos Richard A. ; Swan Dale G. ; Opperman Gary W., Photoactivatable chain transfer agents and semi-telechelic photoactivatable polymers prepared therefrom.
Swan Dale G. (St. Louis Park MN) Josephson Mark W. (Richfield MN) Swanson Melvin J. (Carver MN), Preparation of crosslinked matrices containing covalently immobilized chemical species and unbound releasable chemical s.
Guire Patrick E. (Eden Prairie MN) Dunkirk Shawn G. (Eden Prairie MN) Josephson Mark W. (Richfield MN) Swanson Melvin J. (Carver MN), Preparation of polymeric surfaces via covalently attaching polymers.
Cros Philippe (Lyons FRX) Allibert Patrice A. (Grezieu la Varenne FRX) Mandrand Bernard F. (Villeurbanne FRX) Dalbon Pascal T. (Venissieux FRX), Process for immobilizing a nucleic acid fragment by passive attachment to a solid substrate, the solid substrate thus ob.
Duran Lise W. ; Swanson Melvin J. ; Amos Richard A. ; Hu Sheau-Ping J. ; Guire Patrick E., Reagent and method for attaching target molecules to a surface.
Guire Patrick E. (Eden Prairie MN) Dunkirk Shawn G. (Eden Prairie MN) Josephson Mark W. (Richfield MN) Swanson Melvin J. (Carver MN), Substrate surface preparation.
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