Methods and materials for the detection of Leishmania infection
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/53
G01N-033/542
G01N-033/00
A61K-039/00
C07K-001/00
C07K-014/00
C07K-017/00
출원번호
US-0012398
(2011-01-24)
등록번호
US-8173383
(2012-05-08)
발명자
/ 주소
Houghton, Raymond L.
Reed, Steven G.
Raychaudhuri, Syamal
출원인 / 주소
InBios International, Inc.
대리인 / 주소
Sleath, Janet
인용정보
피인용 횟수 :
0인용 특허 :
2
초록▼
The present invention provides rapid diagnostic assays for the detection of Leishmania which can readily be used in the field, leading to more rapid treatment. In certain embodiments, the inventive assays, including ELISA and lateral flow assays, employ antibodies that may be effectively employed to
The present invention provides rapid diagnostic assays for the detection of Leishmania which can readily be used in the field, leading to more rapid treatment. In certain embodiments, the inventive assays, including ELISA and lateral flow assays, employ antibodies that may be effectively employed to detect the Leishmania major antigen TSA, which is present in both promastigotes grown in culture and in amastigotes. Such assays may be employed to detect the presence of cutaneous leishmaniasis in a subject using scrapings, biopsies, and/or aspirates taken from cutaneous lesions.
대표청구항▼
1. A method for detecting cutaneous leishmaniasis in a biological sample, comprising: (a) contacting the biological sample with a polyclonal antibody specific for a polypeptide of SEQ ID NO: 1 to form an antibody-polypeptide conjugate, wherein the polyclonal antibody is labeled with a reporter agent
1. A method for detecting cutaneous leishmaniasis in a biological sample, comprising: (a) contacting the biological sample with a polyclonal antibody specific for a polypeptide of SEQ ID NO: 1 to form an antibody-polypeptide conjugate, wherein the polyclonal antibody is labeled with a reporter agent;(b) contacting the antibody-polypeptide conjugate with a monoclonal antibody specific for a polypeptide of SEQ ID NO: 1 in order to capture the antibody-polypeptide conjugate, wherein the monoclonal antibody is raised against L. major amastigotes; and(c) detecting the presence of the antibody-polypeptide conjugate, thereby detecting cutaneous leishmaniasis in the biological sample. 2. The method of claim 1, wherein the biological sample is taken from a cutaneous lesion. 3. The method of claim 2, wherein the biological sample is selected from the group consisting of: scrapings, biopsies, and aspirates. 4. The method of claim 1, wherein the biological sample is a mammalian sample. 5. The method of claim 1, wherein the reporter agent is a colorimetric or fluorescent indicator. 6. The method of claim 5, wherein the reporter agent is selected from the group consisting of: colloidal gold and fluorescent dyes. 7. The method of claim 1, wherein the monoclonal antibody is specific for a conformational epitope of the polypeptide of SEQ ID NO: 1 that is present in both amastigotes and promastigotes. 8. The method of claim 1, wherein the presence of the polypeptide of SEQ ID NO: 1 is detected by means of ELISA. 9. The method of claim 1, wherein the monoclonal antibody is immobilized on a solid support. 10. The method of claim 9, wherein the solid support is a nitrocellulose membrane. 11. A method for detecting cutaneous leishmaniasis in a biological sample, comprising: (a) contacting the biological sample with a monoclonal antibody specific for a polypeptide of SEQ ID NO: 1 to form an antibody-polypeptide conjugate, wherein the monoclonal antibody is raised against L. major amastigotes and wherein the monoclonal antibody is labeled with a reporter agent;(b) contacting the antibody-polypeptide conjugate with a polyclonal antibody specific for a polypeptide of SEQ ID NO: 1 in order to capture the antibody-polypeptide conjugate; and(c) detecting the presence of the antibody-polypeptide conjugate, thereby detecting cutaneous leishmaniasis in the biological sample. 12. The method of claim 11, wherein the biological sample is taken from a cutaneous lesion. 13. The method of claim 12, wherein the biological sample is selected from the group consisting of: scrapings, biopsies, and aspirates. 14. The method of claim 11, wherein the biological sample is a mammalian sample. 15. The method of claim 11, wherein the reporter agent is a colorimetric or fluorescent indicator. 16. The method of claim 15, wherein the reporter agent is selected from the group consisting of: colloidal gold and fluorescent dyes. 17. The method of claim 11, wherein the monoclonal antibody is specific for a conformational epitope of the polypeptide of SEQ ID NO: 1 that is present in both amastigotes and promastigotes. 18. The method of claim 11, wherein the presence of the polypeptide of SEQ ID NO: 1 is detected by means of ELISA. 19. The method of claim 11, wherein the polyclonal antibody is immobilized on a solid support. 20. The method of claim 19, wherein the solid support is a nitrocellulose membrane.
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이 특허에 인용된 특허 (2)
Steven G. Reed ; Antonio Campos-Neto ; John R. Webb CA; Davin C. Dillon ; Yasir A. W. Skeiky, Leishmania antigens for use in the therapy and diagnosis of Leishmaniasis.
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