Methods of controlling proliferation and differentiation of stem and progenitor cells
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/06
C12N-005/08
C12N-005/00
출원번호
US-0899268
(2010-10-06)
등록번호
US-8202724
(2012-06-19)
발명자
/ 주소
Peled, Tony
Fibach, Eitan
Treves, Avi
출원인 / 주소
Gamida Cell Ltd.
대리인 / 주소
Mintz Levin Cohn Ferris Glovsky and Popeo, P.C.
인용정보
피인용 횟수 :
0인용 특허 :
186
초록▼
A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for per
A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate.
대표청구항▼
1. A method of expanding a population of mesenchymal stem or progenitor cells, the method comprising providing the cells with conditions for cell proliferation and, at the same time, reducing a capacity of said cells in utilizing copper. 2. The method of claim 1, wherein reducing said capacity of th
1. A method of expanding a population of mesenchymal stem or progenitor cells, the method comprising providing the cells with conditions for cell proliferation and, at the same time, reducing a capacity of said cells in utilizing copper. 2. The method of claim 1, wherein reducing said capacity of the cells in utilizing copper is effected by exposure to a transition metal chelator which binds copper. 3. The method of claim 2, wherein said transition metal chelator is selected from the group consisting of polyamine chelating agents, ethylendiamine, diethylenetriamine, triethylenetetramine, triethylenediamine, tetraethylenepentamine, aminoethylethanolamine, aminoethylpiperazine, pentaethylenehexamine, triethylenetetramine-hydrochloride, tetraethylenepentamine-hydrochloride, pentaethylenehexamine-hydrochloride, tetraethylpentamine, captopril, penicilamine, N,N′-bis(3-aminopropyl)-1,3-propanediamine, N,N,Bis (2 animoethyl) 1,3 propane diamine, 1,7-dioxa-4,10-diazacyclododecane, 1,4,8,11-tetraaza cyclotetradecane-5,7-dione, 1,4,7-triazacyclononane trihydrochloride, 1-oxa-4,7,10-triazacyclododecane, 1,4,8,12-tetraaza cyclopentadecane, 1,4,7,10-tetraaza cyclododecane. 4. The method of claim 3, wherein providing the cells with said conditions for cell proliferation include providing the cells with nutrients and with cytokines. 5. The method of claim 1, wherein said cells are derived from bone marrow. 6. The method of claim 1, wherein said cells are selected from the group consisting of non-differentiated stem cells and committed progenitor cells.
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