IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0286904
(2011-11-01)
|
등록번호 |
US-8211309
(2012-07-03)
|
발명자
/ 주소 |
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
12 인용 특허 :
34 |
초록
▼
A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multis
A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
대표청구항
▼
1. A method of separating proteins from intact algal cells, comprising: providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids;dewatering the wet algal biomass by removing extracel
1. A method of separating proteins from intact algal cells, comprising: providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids;dewatering the wet algal biomass by removing extracellular water to increase the solid content of the wet algal biomass to between 5% and 50% to result in a partially dewatered algal biomass comprising intact algal cells;mixing the partially dewatered wet algal biomass with an amphipathic solvent set in a ratio of about one part amphipathic solvent set to about one part partially dewatered wet algal biomass by weight, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the water, amphipathic solvent set, partially dewatered wet algal biomass that comprises intact algal cells, and proteins that are soluble in a mixture of an amphipathic solvent and water, and the lighter phase comprises the hydrophobic solvent set and polar lipids;separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture;separating the partially dewatered wet algal biomass from the heavier phase of the extraction mixture to generate a protein mixture comprising proteins that are soluble in a mixture of an amphipathic solvent and water, water, and the amphipathic solvent set; andseparating the proteins of the protein mixture from at least a portion of the water and amphipathic solvent to generate a protein fraction, wherein the protein mixture has a higher concentration of proteins that are soluble in a mixture of an amphipathic solvent and water than the separated partially dewatered wet algal biomass. 2. The method of claim 1 wherein dewatering is by centrifuging, filtering, settling or float fractionating. 3. The method of claim 1 wherein the amphipathic solvent set is added in an amount to adjust the polarity index of the extraction mixture to between about 6.5 to 6.7 prior to the addition of the hydrophobic solvent set. 4. The method of claim 3 wherein the hydrophobic solvent set is added in an amount to adjust the polarity index of the extraction mixture to between about 5.7-5.9 after the addition of the amphipathic solvent set. 5. The method of claim 1 wherein the amphipathic solvent set is acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. 2-propanol, acetonitrile, t-butyl alcohol, 1-propanol, water, heavy (D2O), ethylene glycol, glycerin or a combination thereof. 6. The method of claim 1 wherein the hydrophobic solvent set is propane, butane, pentane, butene, propene, naphtha, an alkane, hexane, pentane, heptane, octane, an ester, ethyl acetate, butyl acetate, a ketone, methyl ethyl ketone, methyl isobutyl ketone, an aromatic, toluene, benzene, cyclohexane, tetrahydrofuran, a haloalkane, chloroform, trichloroethylene, an ether, diethyl ether, diesel, jet fuel, gasoline and mixtures thereof. 7. The method of claim 1, wherein the heavier phase is separated from the lighter phase by decanting, membrane filtering or centrifuging. 8. The method of claim 1, wherein the wet algal biomass is separated from the mixture of algal biomass with an amphipathic solvent set, protein and water by decanting, membrane filtering or centrifuging. 9. The method of claim 1 wherein the amphipathic solvent set and water are removed from the protein mixture by evaporation or distillation. 10. The method of claim 1 further comprising recovering the amphipathic solvent set. 11. The method of claim 1 further comprising condensing and recovering the amphipathic solvent set. 12. The method of claim 1, wherein the extraction mixture is heated. 13. The method of claim 12, wherein the mixture is heated with microwaves, water, steam, or hot oil or electricity. 14. The method of claim 12, wherein the extraction mixture is heated at atmospheric pressure. 15. The method of claim 12, wherein the extraction mixture is heated in a pressurized reactor. 16. The method of claim 15, wherein the pressurized reactor is a batch or a continuous reactor. 17. A method of separating proteins from intact algal cells, comprising: providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids;mixing the wet algal biomass with an amphipathic solvent set in a ratio of about one part amphipathic solvent to about one part partially dewatered wet algal biomass by weight, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the water, amphipathic solvent set, wet algal biomass that comprises intact algal cells, and proteins that are soluble in a mixture of an amphipathic solvent and water, and the lighter phase comprises the hydrophobic solvent set and polar lipids;separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture;separating the wet algal biomass from the heavier phase of the extraction mixture to generate a protein mixture comprising proteins that are soluble in a mixture of an amphipathic solvent and water, water, and amphipathic solvent set; andseparating the proteins of the protein mixture to generate a protein fraction, wherein the protein mixture has a higher concentration of proteins that are soluble in a mixture of an amphipathic solvent and water than the separated partially dewatered wet algal biomass.
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