Method for producing biomaterials from bone tissue and material used for osteoplasty and tissue engineering
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-035/32
A61F-002/28
출원번호
US-0091741
(2005-10-27)
등록번호
US-8241673
(2012-08-14)
국제출원번호
PCT/RU2005/000526
(2005-10-27)
§371/§102 date
20080425
(20080425)
국제공개번호
WO2007/049988
(2007-05-03)
발명자
/ 주소
Panasyuk, Andrey Fedorovich
출원인 / 주소
Savaschuk, Dmitry Alekseevich
대리인 / 주소
Bardmesser Law Group
인용정보
피인용 횟수 :
0인용 특허 :
5
초록▼
A method for obtaining of biomaterials for osteoplasty and tissue engineering cleans a bone of natural origin, which is sawn to plates with a thickness of 0.2 to 2.0 cm, washed twice in 0.1 M phosphate buffer at 65° C., pH 5.8-6.0, calculated as two volumetric parts of the buffer solution per one pa
A method for obtaining of biomaterials for osteoplasty and tissue engineering cleans a bone of natural origin, which is sawn to plates with a thickness of 0.2 to 2.0 cm, washed twice in 0.1 M phosphate buffer at 65° C., pH 5.8-6.0, calculated as two volumetric parts of the buffer solution per one part of bone, digested in a solution of activated 0.1-0.4% papain at 65° C. for 24 hours. The plates are washed with 5 volumes of water at the temperature 40-80° C., treated with 0.4 N alkali solution at room temperature for 10-24 hours, washed with flowing water, dried, defatted in ethanol/chloroform mixtures in proportion of 1:2 and then in proportion of 2:1, decalcified in 0.4-1 N hydrochloric acid, treated with 1.5-3% hydrogen peroxide for 4 hours, washed with treated water, then washed with ethanol, dried at room temperature, packed and sterilized.
대표청구항▼
1. A method for obtaining bone plates, from tissue of a bone, the method comprising: (a) cleaning the bone;(b) sawing the bone into plates having a thickness of 0.2-2.0 cm;(c) washing the plates twice in a 0.1 M phosphate buffer solution having a pH of 5.8-6.0 and a temperature of 65° C., wherein th
1. A method for obtaining bone plates, from tissue of a bone, the method comprising: (a) cleaning the bone;(b) sawing the bone into plates having a thickness of 0.2-2.0 cm;(c) washing the plates twice in a 0.1 M phosphate buffer solution having a pH of 5.8-6.0 and a temperature of 65° C., wherein the ratio of bone plates to buffer solution is 1:2 by volume;(d) immersing the plates in a 0.1-0.4% solution of activated papain at 65° C. for 24 hours;(e) washing the plates five times with water having a temperature 40-80° C.;(f) treating the plates with 0.4 N alkali solution at room temperature for 10-24 hours;(g) washing the plates with water;(h) drying the plates;(i) removing fat from the plates by washing with an ethanol and chloroform mixture, wherein the ratio of ethanol to chloroform is 2:1 by volume;(j) removing fat from the plates by washing with an ethanol and chloroform mixture, wherein the ratio of ethanol to chloroform is 1:2 by volume;(k) decalcifying the plates with a solution of hydrochloric acid;(l) treating the plates with a solution of hydrogen peroxide for 4 hours;(m) washing the plates with water at room temperature until the hydrogen peroxide is removed from the plates;(n) washing the plates with ethanol;(o) drying the plates;(p) packing the plates; and(q) sterilizing the plates. 2. A method for obtaining bone fragments from tissue of a bone, the method comprising: (a) sawing the bone into fragments having a thickness of 0.2-2.0 cm;(b) washing the fragments in a 0.1 M phosphate buffer solution having a pH of 5.8-6.0 and a temperature of 65° C.;(c) immersing the fragments in a solution of activated papain;(d) washing the fragments with water;(e) treating the fragments with a 0.4 N alkali solution at room temperature;(f) washing the fragments with water;(g) drying the fragments;(h) removing fat from the fragments by washing with an ethanol/chloroform mixture;(i) decalcifying the fragments with a solution of an acid;(j) treating the fragments with hydrogen peroxide;(k) washing the fragments with water until the hydrogen peroxide is removed from the fragments;(l) washing the fragments with ethanol; and(m) drying the fragments. 3. The method of claim 1, wherein the drying in step (g) includes freeze-drying. 4. The method of claim 1, wherein the papain solution is 0.1-0.4% papain. 5. The method of claim 1, wherein the papain solution is at 65° C. 6. The method of claim 1, wherein the immersing step lasts for about 24 hours. 7. The method of claim 1, wherein step (d) is performed multiple times. 8. The method of claim 1, wherein the water in step (d) has a temperature of 40-80° C. 9. The method of claim 1, wherein step (e) is performed for 10-24 hours. 10. The method of claim 1, wherein the ethanol and chloroform mixture of step (h) has a 2:1 ratio by volume of ethanol to chloroform. 11. The method of claim 10, wherein in step (h) the bone fragments are further washed with an ethanol and chloroform mixture having a 1:2 ratio by volume of ethanol to chloroform. 12. The method of claim 1, wherein the acid in step (i) is hydrochloric acid. 13. The method of claim 12, wherein the hydrochloric acid concentration is 0.4-1.0 N. 14. The method of claim 1, wherein step (j) is performed for 4 hours. 15. The method of claim 1, wherein the water in step (k) is at room temperature. 16. The method of claim 1, further comprising the steps of: (n) packing the fragments; and(o) sterilizing the fragments. 17. The method of claim 1, wherein the fragments are any of plates, cubes, parallelepipeds and blocks. 18. The method of claim 1, further comprising cleaning the bone prior to step (a). 19. The method of claim 1, wherein in step (b) the ratio of bone fragments to buffer solution is 1:2 by volume.
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이 특허에 인용된 특허 (5)
Jefferies Steven R. (5802 Leith Walk Baltimore MD 21239), Bone graft material for osseous defects and method of making same.
Campbell Todd D. (Corona CA) Nashef Aws S. (Costa Mesa CA), Method of producing prostheses for replacement of articular cartilage and prostheses so produced.
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