A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which result
A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which results in the formation of lyso-phospholipids and free fatty acids. The diacylglycerols and the free fatty acids from these two separate reactions then combine in the presence of the enzymes to generate new triacylglycerol molecules.
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1. A method of generating triacylglycerols from oil gums, the method comprising (a) providing an oil composition containing a quantity of oil gums, said gums comprising phospholipids,(b) separating said oil gums from said oil composition to provide a first fraction substantially free of oil gums and
1. A method of generating triacylglycerols from oil gums, the method comprising (a) providing an oil composition containing a quantity of oil gums, said gums comprising phospholipids,(b) separating said oil gums from said oil composition to provide a first fraction substantially free of oil gums and a second fraction containing said separated oil gums,(c) treating said second fraction with one or more enzymes having PLA activity to generate free fatty acids,(d) treating said second fraction with one or more enzymes having PLC activity to generate diacylglycerols,(e) reacting said fatty acids and said diacylglycerols with one another in the presence of at least one of said enzymes having PLA or PLC activity, thereby forming triacylglycerols. 2. The method of claim 1 wherein steps (c) and (d) occur substantially simultaneously. 3. The method of claim 1, wherein step (c) occurs before step (d). 4. The method of claim 1 wherein step (d) occurs before step (c). 5. The method of claim 1, wherein the treating of the second fraction with the enzymes in steps (c) or (d) is no more than about four hours. 6. The method of claim 5, wherein the treating of the second fraction with the enzymes in steps (c) or (d) is about thirty minutes. 7. The method of claim 1, wherein said one or more enzymes having PLA activity are selected from the group consisting of a phospholipase A1 enzyme and a phospholipase A2 enzyme. 8. The method of claim 1, wherein said one or more enzymes having PLC activity are selected from the group consisting of a phospholipase C enzyme and a phosphatidyl-inositol specific phospholipase C enzyme. 9. The method claim 1, wherein said treating of steps (c) or (d) or the reacting of step (e) comprises treating or reacting at a pH of about 8 or less. 10. The method claim 9, wherein said pH is a pH of about 3-7. 11. The method of claim 1, wherein said reaction of the enzymes with the second fraction occurs at a temperature of about 40-80° C. 12. The method of claim 11, wherein said reaction of the enzymes with the second fraction occurs at a temperature of about 40-60° C. 13. The method of claim 1, wherein said oil composition comprises a crude oil. 14. The method claim 1, wherein in step (b) the separating of the oil gums from the oil composition is accomplished by a method selected from the group consisting of water degumming, acid degumming, caustic refining, and enzyme degumming, provided that the enzyme of the enzyme degumming is other than a combination of enzymes PLA and PLC. 15. The method claim 14, wherein in step (b) the separating of the oil gums from the oil composition is accomplished by caustic refining, and wherein before the treating of steps (c) or (d) the separated gums in the caustic refined second fraction are brought to a pH of about 8 or less. 16. The method of claim 1, wherein in steps (d) or (e) said PLC enzyme is present in a quantity of about 30 ppm of active enzyme or less. 17. The method of claim 16, wherein said PLC enzyme is present in a quantity of about 20 ppm of active enzyme or less. 18. The method of claim 17, wherein said PLC enzyme is present in a quantity of about 10 ppm of active enzyme or less. 19. The method of claim 1, wherein in steps (c) or (e) said PLA enzyme is present in a quantity of about 2 ppm of active enzyme or less. 20. The method of claim 19, wherein said PLA enzyme is present in a quantity of about 1 ppm of active enzyme or less. 21. The method of claim 20, wherein said PLA enzyme is present in a quantity of about 0.5 ppm of active enzyme or less. 22. The method claim 1, wherein the treating of steps (c) or (d) comprise shear mixing the second fraction and the respective PLA or PLC enzymes. 23. The method of claim 22, wherein said shear mixing continues for a duration of at least 45 seconds. 24. The method claim 22, wherein the treating of steps (c) or (d) further comprises adding a quantity of water to the mixture. 25. The method of claim 24, wherein said quantity of water is at least about 1.5% by weight of the total mixture. 26. The method of claim 25, wherein said quantity of water is at least about 3.0% by weight of the total mixture. 27. The method of claim 26, wherein said quantity of water is at least about 4.5% by weight of the total mixture.
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