초록

Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

대표청구항 ▼

1. A multi-enzyme product comprising isolated enzymes from a Trichoderma and exhibiting: a glucoamylase activity of at least about 0.001 U per milligram of dry matter, a β-xylosidase activity of at least about 280 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 454 nmol/min/mg

1. A multi-enzyme product comprising isolated enzymes from a Trichoderma and exhibiting: a glucoamylase activity of at least about 0.001 U per milligram of dry matter, a β-xylosidase activity of at least about 280 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 454 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 100 nmol/min/mg of dry matter, wherein the multi-enzyme product increases the yield of fermentable sugars from Distiller's Dried Grains (DDG) over compositions not including the claimed amount of glucoamylase. 2. The multi-enzyme product of claim 1, wherein the product is a crude fermentation product. 3. The multi-enzyme product of claim 1, wherein the product has been subjected to a purification step. 4. The multi-enzyme product of claim 1, wherein the Trichoderma is Trichoderma reesei. 5. The multi-enzyme product of claim 1, wherein the product is capable of converting lignocellulosic material to sugars. 6. The multi-enzyme product of claim 1, wherein the fermentable sugars comprise glucose. 7. The multi-enzyme product of claim 1, wherein the DDG is derived from corn.