초록 ▼

Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include e

Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.

대표청구항 ▼

1. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising: a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample

1. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising: a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and including an anchoring element including a surface facing the bottom wall to anchor the separating structure in embedding material during a microtome sectioning process; andtissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 2. The histologic tissue sample support device of claim 1, wherein the biasing structure further comprises a resilient cellular material configured to engage the tissue sample and hold the tissue sample flat against a reference surface, the resilient cellular material being porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material used to embed the tissue while the tissue sample is retained by the resilient cellular material. 3. The histologic tissue sample support device of claim 2, wherein the resilient cellular material further comprises an open cell foam material. 4. The histologic tissue sample support device of claim 3, wherein the open cell foam material further includes at least one of a polyether or a polyurethane. 5. The histologic tissue sample support device of claim 3, wherein the open cell foam is a fully reticulated foam. 6. The histologic tissue sample support device of claim 1, further comprising: fingers on opposite sides of the first and second channels to hold the tissue sample or samples in a desired orientation. 7. The histologic tissue sample support device of claim 6, wherein the cassette is formed of a polymer. 8. The histologic tissue sample support device of claim 7, wherein the polymer is a fluorinated polymer. 9. The histologic tissue sample support device of claim 7, wherein the polymer is a fluoropolymer. 10. The histologic tissue sample support of claim 1, wherein the cassette includes a lid and the biasing structure further comprises a plurality of spaced apart tabs connected with and extending downwardly from the lid into the first and second channels. 11. The histologic tissue sample support of claim 1, wherein the separating structure is perforated. 12. The histologic tissue sample support of claim 1, wherein the separating structure is configured to allow the tissue sample to extend from the first channel to the second channel. 13. A histologic tissue sample support device, comprising: a frame;a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and including an anchoring element including a surface facing the bottom wall to anchor the separating structure in embedding material during a microtome sectioning process; andtissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples in place during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 14. The histologic tissue sample support device of claim 13, wherein the tissue cassette is configured to be releasably coupled to the frame and the frame is further configured for releasable securement within a microtome chuck. 15. The histologic tissue sample support device of claim 14, wherein the frame includes an interior and the tissue cassette is sized to fit and move within the interior between at least a first position and a second position, the first position being used during processing of the tissue sample, and the second position being used to expose the tissue outward of the frame in a position for allowing the tissue sample to be sectioned in the microtome. 16. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising: a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and configured to allow the tissue sample to extend from the first channel to the second channel; andtissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 17. A histologic tissue sample support device, comprising: a frame;a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and configured to allow the tissue sample to extend from the first channel to the second channel; andtissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples in place during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 18. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising: a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including an elongate channel for receiving at least one tissue sample, the channel including resilient fingers on opposite sides thereof for resiliently engaging and holding the tissue sample during processing and embedding; andtissue sample biasing structure configured to extend into the channel to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 19. The histologic tissue sample support device of claim 18 wherein the resilient fingers have a one-way barbed-type configuration to prevent the tissue sample from backing out of the channel. 20. A histologic tissue sample support device, comprising: a frame;a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including an elongate channel for receiving at least one tissue sample, the channel including resilient fingers on opposite sides thereof for resiliently engaging and holding the tissue sample during processing and embedding; andtissue sample biasing structure configured to extend into the channel to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. 21. The histologic tissue sample support device of claim 20 wherein the resilient fingers have a one-way barbed-type configuration to prevent the tissue sample from backing out of the channel.