Apparatus and method for detecting and quantifying analytes in solution
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01J-005/02
G01J-005/00
출원번호
US-0196340
(2011-08-02)
등록번호
US-8344323
(2013-01-01)
발명자
/ 주소
Hartley, Frank Thomas
출원인 / 주소
Roc8Sci Co.
대리인 / 주소
Kaplan Breyer Schwarz & Ottesen, LLP
인용정보
피인용 횟수 :
2인용 특허 :
3
초록▼
A method for identifying and quantifying one or more analytes included in a sample comprising a background solvent is disclosed. The present invention enables in-situ calibration and removal of the spectral signature of the background solvent from a composite spectrum so that the spectral features a
A method for identifying and quantifying one or more analytes included in a sample comprising a background solvent is disclosed. The present invention enables in-situ calibration and removal of the spectral signature of the background solvent from a composite spectrum so that the spectral features associated with the analyte(s) can be more easily and precisely identified. Further, the method enables estimation of the concentration of the analyte(s) by normalizing the spectrum based on the path length of the infrared radiation through the sample.
대표청구항▼
1. An apparatus comprising a spectrometer, the spectrometer comprising: (1) a source of mid-infrared radiation that is within the wavelength range of approximately 2.5 microns to approximately 12.5 microns, the source being dimensioned and arranged to enable illumination of a sample with the infrare
1. An apparatus comprising a spectrometer, the spectrometer comprising: (1) a source of mid-infrared radiation that is within the wavelength range of approximately 2.5 microns to approximately 12.5 microns, the source being dimensioned and arranged to enable illumination of a sample with the infrared radiation;(2) a wavelength separator, the wavelength separator receiving a first composite signal from the sample, the first composite signal being based on the infrared radiation and spectral characteristics of the sample, wherein the wavelength separator distributes the first composite signal into a plurality of wavelength signals; and(3) a plurality of detectors, wherein each of the plurality of detectors generates a first electrical signal based on one of the first plurality of wavelength signals, and wherein each of the plurality of detectors comprises; (i) a plate, wherein the plate comprises an absorber that converts incident light to heat;(ii) an electrode disposed on a substrate, the electrode and the plate collectively defining a parallel-plate capacitor whose capacitance is based on a first separation between the electrode and the plate; and(iii) an actuator, the actuator being dimensioned and arranged to change the first separation in response to a change in the temperature of the actuator;wherein the plate and the actuator are thermally coupled to enable transfer of heat from the plate to the actuator, and wherein the actuator and the substrate are thermally coupled to enable transfer of heat from the actuator to the substrate. 2. The apparatus of claim 1, each actuator comprising a first bi-material element having a first end and a second end, the first end being mechanically coupled with the plate and the second end being substantially immovable with respect to the substrate, wherein a change in the temperature of the first bi-material element induces motion of the first end with respect to the substrate. 3. The apparatus of claim 2 wherein the first material comprises diamond. 4. The apparatus of claim 2, each actuator comprising a second bi-material element having a third end and a fourth end, the third end being mechanically coupled with the plate and the fourth end being substantially immovable with respect to the substrate, wherein a change in the temperature of the second bi-material element induces motion of the third end with respect to the substrate, and wherein the plate, first bi-material element, and second bi-material element are dimensioned and arranged such that motion of the first end and third end induces the plate to move with respect to the electrode while remaining substantially parallel with the electrode. 5. The apparatus of claim 1 wherein the substrate is electrically conductive. 6. The apparatus of claim 1 wherein the source is dimensioned and arranged to emit the infrared radiation as a pulse of infrared radiation. 7. The apparatus of claim 6 wherein the source emits the infrared radiation in response to a drive signal that is an electrical pulse. 8. The apparatus of claim 1 further comprising: (4) a processor; and(5) a database comprising a plurality of spectra for each of a first chemical constituent and a second chemical constituent, wherein the sample comprises the first chemical constituent and the second chemical constituent;wherein the processor is dimensioned and arranged to receive the plurality of wavelength signals and provide an output signal that is based on the plurality of wavelength signals and the plurality of spectra. 9. A method comprising: providing a first composite spectral signal that is based on a first sample comprising a first chemical constituent and a second chemical constituent, wherein the first composite spectral signal includes a first spectral signal and a second spectral signal, the first spectral signal being based on the first chemical constituent and the second spectral signal being based on the second chemical constituent;providing a first spectral model that is based on spectra corresponding to the first chemical constituent and spectra corresponding to the second chemical constituent;processing the first composite spectral signal and the first spectral model to reduce the magnitude of the first spectral signal; and estimating a first concentration of the second chemical constituent in the first sample. 10. The method of claim 9 further comprising providing wavelength registration for the first composite signal based a wavelength reference, wherein the wavelength reference is based on temperature-related changes in a plurality of spectral peaks in the spectra corresponding to the first chemical constituent. 11. The method of claim 9 wherein the first composite spectral signal is provided by operations comprising: providing a first composite light signal by exposing the first sample to infrared radiation that comprises a first range of wavelengths, wherein the first composite light signal comprises spectral information that is based on the first sample;distributing the first composite light signal into a plurality of wavelength signals, wherein the plurality of wavelength signals collectively span the first range of wavelengths; andreceiving each of the plurality of wavelength signals at a different one of a plurality of detectors, wherein the plurality of detectors collectively generates the first composite spectral signal. 12. The method of claim 11 wherein each of the plurality of detectors generates a portion of the first composite spectral signal by operations comprising: receiving one of the plurality of wavelength signals at a plate comprising an absorber, wherein the absorber converts received wavelength signal into heat, and wherein the heat is based on the intensity of the wavelength signal;controlling a separation between the plate and an electrode, wherein the plate and the electrode collectively define a capacitor whose capacitance is based on the separation, and wherein the separation is based on the temperature of an actuator that is thermally coupled with the plate; andenabling the flow of heat from the actuator;wherein the portion of the first composite spectral signal is based on the capacitance of the capacitor. 13. The method of claim 9 further comprising normalizing the first composite spectral signal, wherein the first composite spectral signal is normalized based on the spectra corresponding to each of the first chemical constituent, and wherein the first concentration for the second chemical constituent is estimated based on the magnitude of the normalized first composite spectral signal. 14. The method of claim 13 wherein the first sample further comprises a third chemical constituent, and wherein the first composite spectral signal further includes a third spectral signal that is based on the third chemical constituent, and wherein the first spectral model is based further on spectra corresponding to the third chemical constituent, and further wherein the method further comprises estimating a second concentration for the third chemical constituent in the first sample based on the magnitude of the normalized first composite signal. 15. The method of claim 9 further comprising providing the first spectral model, wherein the first spectral model is provided by operations comprising: generating a plurality of first spectra, wherein each of the plurality of first spectra is based substantially only the first chemical constituent and wherein each of the plurality of first spectra is based on the first chemical constituent at a different temperature;storing the plurality of first spectra in a database;generating a plurality of second spectra, wherein each of the plurality of second spectra is generated by measuring a different one of a plurality of third samples that consist essentially of the first chemical constituent and the second chemical constituent, and wherein each of the plurality of third samples has a different concentration of the second chemical constituent;determining a best-fit first spectrum for each of the plurality of second spectra;normalizing each of the plurality of second spectra based on its corresponding best-fit first spectrum;modifying each of the plurality of second spectra by substantially removing its corresponding best-fit first spectrum; andstoring the plurality of modified second spectra in the database. 16. The method of claim 15 further comprising establishing a wavelength reference based on a temperature dependence of at least one wavelength peak that is included in each of the plurality of first spectra. 17. The method of claim 16 wherein the temperature dependence of the at least one wavelength peak is based on stretch-and-bend vibrations in the first chemical constituent. 18. A method comprising: providing a first composite light signal by exposing a first sample to mid-infrared radiation that comprises a first range of wavelengths, wherein the first sample comprises a first chemical constituent and a second chemical constituent, and wherein the first composite light signal comprises spectral information that is based each of the first chemical constituent and the second chemical constituent;distributing the first composite light signal into a plurality of wavelength signals, wherein the plurality of wavelength signals collectively span the first range of wavelengths; andproviding a first composite spectral signal that comprises a plurality of first output signals, wherein each of the plurality of output signals is provided by operations comprising:receiving each of the plurality of wavelength signals at a different one of a plurality of detectors, wherein each of the detectors provides a first output signal that is based on the intensity of its received wavelength signal, and wherein each of the detectors provides the first output signal by operations comprising;converting incident light into heat;conveying at least a portion of the heat to an actuator, wherein the actuator controls the capacitance of a capacitor based on the temperature of the actuator, wherein the actuator is thermally coupled with a substrate such that the flow of heat from the actuator to the substrate is facilitated; andproviding the first output signal based on the capacitance of the capacitor. 19. The method of claim 18 further comprising providing the infrared radiation, wherein the infrared radiation is provided by operations comprising: providing a first electrical signal to a source, wherein the source is dimensioned and arranged to convert electrical energy into infrared radiation characterized by the first range of wavelengths; andmodulating the first electrical signal such that the first electrical signal comprises a plurality of pulses of electrical energy. 20. The method of claim 18 further comprising generating a second output signal that is based on first composite spectral signal and a first spectral model, wherein the first spectral model is based on a plurality of first spectra that are based on substantially only the first chemical constituent and a plurality of second spectra that are based on the second chemical constituent. 21. The method of claim 20 further comprising providing the first spectral model by operations comprising: generating the plurality of first spectra, wherein each of the plurality of first spectra is based substantially only the first chemical constituent and wherein each of the plurality of first spectra is based on the first chemical constituent at a different temperature;storing the plurality of first spectra in a database;generating the plurality of second spectra, wherein each of the plurality of second spectra is generated by measuring a different one of a plurality of third samples that consist essentially of the first chemical constituent and the second chemical constituent, and wherein each of the plurality of third samples has a different concentration of the second chemical constituent;determining a best-fit first spectrum for each of the plurality of second spectra;normalizing each of the plurality of second spectra based on its corresponding best-fit first spectrum;modifying each of the plurality of second spectra by substantially removing its corresponding best-fit first spectrum; andstoring the plurality of modified second spectra in the database.
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