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A method for producing an image of an object within a chamber of a microfluidic device includes providing the microfluidic device having x, y, and z dimensions and a chamber depth center point located along the z dimension. The chamber depth center point is located a known z dimension distance from

A method for producing an image of an object within a chamber of a microfluidic device includes providing the microfluidic device having x, y, and z dimensions and a chamber depth center point located along the z dimension. The chamber depth center point is located a known z dimension distance from a fiducial marking embedded within the microfluidic device. The method also includes placing the microfluidic device within an imaging system that includes an optical device capable of detecting the fiducial marking. The optical device defines an optical path axially aligned with the z dimension and has a focal plane perpendicular to the optical path. When the focal plane is moved along the optical path, the fiducial marking is maximally detected when the focal plane is at the z depth in comparison to when the focal plane is not substantially in-plane with the z depth.

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1. A method for producing an image of an object within a chamber of a microfluidic device comprising the steps of: providing said microfluidic device, said microfluidic device having x, y, and z dimensions and further comprising a chamber depth center point located between a top wall and a bottom wa

1. A method for producing an image of an object within a chamber of a microfluidic device comprising the steps of: providing said microfluidic device, said microfluidic device having x, y, and z dimensions and further comprising a chamber depth center point located between a top wall and a bottom wall of said chamber along said z dimension, said chamber depth center point being located a known z dimension distance from an optically detectable fiducial marking embedded within said microfluidic device at a z depth;placing said microfluidic device within an imaging system comprising: an optical device capable of detecting said fiducial marking and transmitting said image of said object, said optical device defining an optical path axially aligned with said z dimension of said microfluidic device and having a focal plane perpendicular to said optical path, wherein when said focal plane is moved along said optical path in line with said fiducial marking, said fiducial marking is maximally detected when said focal plane is at said z depth in comparison to when said focal plane is not substantially in-plane with said z depth,an image processing device in communication with said optical device, said image processing device being able to control said optical device to cause said focal plane to move along said z axis and move said focal plane to maximally detect said fiducial marking, said image processing device being further able to transmit said image of said object;controlling said optical device with said image processing device to cause said focal plane to move along said optical path until said optical device maximally detects said fiducial marking;further controlling said optical device with said image processing device to move said focal plane along said optical path said z dimension distance to cause said field depth center point to be located at said chamber depth center point; and,imaging said object within said chamber while said focal plane is located said chamber depth center point. 2. The method of claim 1 wherein said microfluidic device is made wholly or partly from an elastomeric material. 3. The method of claim 2 wherein said elastomeric material is polydimethlysiloxane. 4. The method of claim 1 wherein said microfluidic device is partly made from glass material. 5. The method of claim 4 wherein said chamber is formed wholly or partly in said glass material. 6. The method of claim 2 wherein said chamber is wholly or partly within said elastomeric material. 7. The method of claim 1 wherein said depth of field is greater than, equal to, or less than the z dimension of said chamber. 8. The method of claim 1 wherein said optical device comprises an analog output charged coupled device type image detector and said image processor comprises an analog to digital converter. 9. The method of claim 1 wherein said optical device comprises a digital detection device. 10. The method of claim 1 wherein said image processing device comprises a digital computer and a data storage device. 11. The method of claim 10 wherein said digital computer comprises an output display for displaying said image of said object. 12. A system for producing an image of an object within a chamber of a microfluidic device comprising: said microfluidic device, said microfluidic device having x, y, and z dimensions and further comprising a chamber depth center point located between a top wall and a bottom wall of said chamber along said z dimension, said chamber depth center point being located a known z dimension distance from a optically detectable fiducial marking embedded within said microfluidic device at a z depth;an imaging system for placing said microfluidic device therein comprising: an optical device capable of detecting said fiducial marking and transmitting said image of said object, said optical device defining an optical path axially aligned with said z dimension of said microfluidic device and having a focal plane, wherein when said focal plane is moved along said optical path in line with said fiducial marking, said fiducial marking is maximally detected when said focal plane is substantially in-plane with said z depth as compared to when said field depth center point is not substantially in-plane with said z depth,an image processing device in communication with said optical device, said image processing device being able to control said optical device to cause said focal plane to move along said z axis and move said field depth center point to maximally detect said fiducial marking, said image processing device being further able to transmit said image of said object, said image processing device being in operable communication with said optical device to cause said focal plane to move along said optical path until said optical device maximally detects said fiducial marking,wherein when said image processing device causes said optical device to move said focal plane along said optical path said z dimension distance, said focal point is located at said chamber depth center point. 13. The system of claim 12 wherein said microfluidic device is made wholly or partly from an elastomeric material. 14. The system of claim 13 wherein said elastomeric material is polydimethylsiloxane. 15. The system of claim 12 wherein said microfluidic device is partly made from glass material. 16. The system of claim 12 wherein said chamber is formed wholly or partly in said glass material. 17. The system of claim 13 wherein said chamber is wholly or partly within said elastomeric material. 18. The system of claim 12 wherein said depth of field is greater than, equal to, or less than the z dimension of said chamber. 19. The system of claim 12 wherein said optical device comprises an analog output charged coupled device type image detector and said image processor comprises an analog to digital converter. 20. The system of claim 12 wherein said optical device comprises a digital detection device. 21. The system of claim 12 wherein said image processing device comprises a digital computer and a data storage device. 22. The system of claim 21 wherein said digital computer comprises an output display for displaying said image of said object. 23. The system of claim 22 wherein said output display comprises a graphical user interface.