Biodegradable polyacetals for in vivo polynucleotide delivery
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-031/74
C07H-001/00
출원번호
US-0879948
(2010-09-10)
등록번호
US-8383091
(2013-02-26)
발명자
/ 주소
Van, Sang
Zhou, Jiadong
Fu, Xiaoli
Yu, Lei
출원인 / 주소
Nitto Denko Corporation
인용정보
피인용 횟수 :
0인용 특허 :
23
초록
Degradable complexes comprising a polycation, a polyanion and a polynucleotide are useful for in vivo polynucleotide delivery applications.
대표청구항▼
1. A polyanion comprising recurring units represented by Formula (I): wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)2—O—(CH2)2—O—(CH2)2—;wherein W is a targeting ligand selected from the group consisting of galactose, lactose, mannose, transferrin, antibod
1. A polyanion comprising recurring units represented by Formula (I): wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)2—O—(CH2)2—O—(CH2)2—;wherein W is a targeting ligand selected from the group consisting of galactose, lactose, mannose, transferrin, antibody, antibody fragment, and RGD peptide;wherein m and n are each individually integers in the range of 1 to 100,000; andwherein Z is selected from the group consisting of Li, Na, K, Rb, Cs, Be, Mg, and Ca. 2. The polyanion of claim 1, wherein Y is —(CH2)2—O—(CH2)2—O—(CH2)2—. 3. The polyanion of claim 1, wherein Y is —(CH2)2—O—(CH2)2—. 4. The polyanion of claim 1, wherein Y is —(CH2)2—. 5. The polyanion of claim 1, wherein Z is Li or Na. 6. The polyanion of claim 1, wherein the polyanion is acid degradable. 7. The polyanion of claim 1, wherein the polyanion has a molecular weight in the range of about 500 Daltons to about 5,000,000 Daltons. 8. A polyanion comprising recurring units represented by formula (II): wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)2—O—(CH2)2—O—(CH2)2—;wherein m and n are each individually integers in the range of 1 to 100,000; andwherein Z is selected from the group consisting of Li, Na, K, Rb, Cs, Be, Mg, and Ca. 9. The polyanion of claim 8, wherein Z is Li or Na. 10. A method for making the polyanion of claim 1, comprising: hydrolyzing a polyacetal to form a hydrolyzed polyacetal; andcoupling a galactosamine with the hydrolyzed polyacetal to form the polyanion of claim 1. 11. The method of claim 10, wherein the polyacetal comprises a recurring unit having the following structure: wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)2—O—(CH2)2—O—(CH2)2—; andwherein m is an integer in the range of 1 to 100,000. 12. The method of claim 11, wherein Y is —(CH2)2—O—(CH2)2—. 13. The method of claim 10, wherein the hydrolyzed polyacetal comprises a recurring unit having the following structure: wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)2—O—(CH2)2—O—(CH2)2—;wherein m is an integer in the range of 1 to 100,000; and wherein Z is selected from the group consisting of Li, Na, K, Rb, Cs, Be, Mg, and Ca. 14. The method of claim 13, wherein Z is Li or Na. 15. The method of claim 13, wherein Y is —(CH2)2—O—(CH2)2—. 16. The method of claim 10 in which the coupling is conducted using a coupling reagent selected from the group consisting of DIC, NHS and DMAP. 17. The method of claim 10 in which the coupling is conducted using DMAP.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (23)
Van,Sang; Viroonchatapan,Nitnara; Ji,Shouping; Yu,Lei, Acid-sensitive polyacetals and methods.
Robert Allen Snow ; Paul Mark Henrichs ; Daniel Joseph Delecki ; William Anthony Sanderson ; Vinay Chandrakant Desai ; Edward Bacon ; Kenneth Robert Hollister ; Eric Paul Hohenschuh, Compounds.
Saudek Vladimr (Prague CSX) Rypacek Frantisek (Prague CSX) Drobnik Jaroslav (Prague CSX), Soluble and biodegradable polyamino acid activated for bonding of biologically active compound.
Hata Tadashige (Kurashiki JPX) Matsuzaki Kazuhiko (Kurashiki JPX) Yahiro Shiyuzi (Kurashiki JPX), Thermoplastic graft copolymer and process for producing the same.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.