Method for diagnosis and monitoring of viral infection by analysis of viral transrenal nucleic acids in urine
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12P-019/34
C07H-021/02
C07H-021/04
출원번호
US-0114289
(2011-05-24)
등록번호
US-8383335
(2013-02-26)
우선권정보
IT-RM2005A0067 (2005-02-17)
발명자
/ 주소
Melkonyan, Hovsep
Cannas, Angela
Tomei, Louis David
Umansky, Samuil R.
출원인 / 주소
Trovagene, Inc.
인용정보
피인용 횟수 :
0인용 특허 :
7
초록▼
The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is p
The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is performed through hybridization of the nucleic acids with specific probes, or through a chain amplification reaction with specific primers. The methods are applicable to all viral pathogenic agents, including RNA, DNA, episomal, or integrated viruses.
대표청구항▼
1. A method for diagnosing a virus infection in a subject, comprising a) obtaining a urine sample from a subject;b) separating a soluble fraction of said urine sample; andc) detecting the presence of a cell-free virus nucleic acid in said soluble fraction of said urine sample, wherein said nucleic a
1. A method for diagnosing a virus infection in a subject, comprising a) obtaining a urine sample from a subject;b) separating a soluble fraction of said urine sample; andc) detecting the presence of a cell-free virus nucleic acid in said soluble fraction of said urine sample, wherein said nucleic acid is less than about 300 bp in length, wherein said detecting is performed by a method selected from the group consisting of PCR, nested PCR, SSCP, LCR, and SDA, using a primer set comprisingat least one forward primer selected from the group consisting of SEQ ID NOs: 1 and 3 and at least one reverse primer selected from the group consisting of SEQ ID NOs: 2 and 4, ora forward primer of SEQ ID NO: 5 and at least one reverse primer selected from the group consisting of SEQ ID NOs: 6 and 7, ora forward primer of SEQ ID NO: 11 and a reverse primer of SEQ ID NO: 10,wherein the presence of said nucleic acid diagnoses a viral infection. 2. The method of claim 1, further comprising the step of quantifying the nucleic acid. 3. The method of claim 1, wherein the subject is a mammal. 4. The method of claim 3, wherein the mammal is a human. 5. A method for diagnosing a virus infection in a subject, comprising a) obtaining a urine sample from a subject;b) separating a soluble fraction of said urine sample; andc) detecting the presence of a cell-free virus nucleic acid in said soluble fraction of said urine sample by a method selected from the group consisting of PCR, nested PCR, SSCP, LCR, and SDA, using a forward primer of SEQ ID NO: 5 and a reverse primer of SEQ ID NO: 8, wherein the presence of said nucleic acid diagnoses a viral infection. 6. The method of claim 5, further comprising the step of quantifying the nucleic acid. 7. The method of claim 5, wherein the subject is a mammal. 8. The method of claim 7, wherein the mammal is a human. 9. A method for diagnosing a virus infection in a subject, comprising a) obtaining a urine sample from a subject;b) separating a soluble fraction of said urine sample; andc) detecting the presence of a cell-free virus nucleic acid in said soluble fraction of said urine sample by a method selected from the group consisting of PCR, nested PCR, SSCP, LCR, and SDA, using a forward primer of SEQ ID NO: 9 and a reverse primer of SEQ ID NO: 10, wherein the presence of said nucleic acid diagnoses a viral infection. 10. The method of claim 9, further comprising the step of quantifying the nucleic acid. 11. The method of claim 9, wherein the subject is a mammal. 12. The method of claim 11, wherein the mammal is a human. 13. The method of claim 2, wherein said primer set comprises at least one forward primer selected from SEQ ID NOs: 1 and 3 and at least one reverse primer selected from SEQ ID NOs: 2 and 4. 14. The method of claim 4, wherein said primer set comprises at least one forward primer selected from SEQ ID NOs: 1 and 3 and at least one reverse primer selected from SEQ ID NOs: 2 and 4. 15. The method of claim 2, wherein said primer set comprises a forward primer of SEQ ID NO: 5 and at least one reverse primer selected from the group consisting of SEQ ID NOs: 6 and 7. 16. The method of claim 4, wherein said primer set comprises a forward primer of SEQ ID NO: 5 and at least one reverse primer selected from the group consisting of SEQ ID NOs: 6 and 7. 17. The method of claim 2, wherein said primer set comprises a forward primer of SEQ ID NO: 11 and a reverse primer of SEQ ID NO: 10. 18. The method of claim 4, wherein said primer set comprises a forward primer of SEQ ID NO: 11 and a reverse primer of SEQ ID NO: 10.
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이 특허에 인용된 특허 (7)
McDonough Sherrol H. ; Ryder Thomas B. ; Yang Yeasing, Detection of human immunodeficiency virus type 1.
Leckie Gregor W. (Highland Park IL) Davis Alan H. (Vernon Hills IL) Semple-Facey Ingrid E. (Beach Park IL) Manlove Matthew T. (Vernon Hills IL) Solomon Natalie A. (Buffalo Grove IL), Materials and methods for the detection of Mycobacterium tuberculosis.
Crawford Jack T. (Atlanta GA) Eisenach Kathleen D. (Little Rock AR) Cave M. Donald (Little Rock AR) Bates Joseph H. (Little Rock AR), Repetitive DNA sequence specific for mycobacterium tuberculosis to be used for the diagnosis of tuberculosis.
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