Method and apparatus for lysing and processing algae
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B01D-011/02
C12N-001/12
B01D-011/04
C02F-001/26
출원번호
US-0083895
(2011-04-11)
등록번호
US-8388846
(2013-03-05)
발명자
/ 주소
Chew, Geoffrey
Reich, Alton J.
Dykes, Jr., H. Waite H.
Di Salvo, Roberto
출원인 / 주소
Streamline Automation, LLC
인용정보
피인용 횟수 :
0인용 특허 :
2
초록▼
Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells at lower temperatures than existing algae processing methods. A salt or salt solution is used as a separation agent and to remove water from the ionic liquid, allowing the ionic l
Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells at lower temperatures than existing algae processing methods. A salt or salt solution is used as a separation agent and to remove water from the ionic liquid, allowing the ionic liquid to be reused. The used salt may be dried or concentrated and reused. The relatively low lysis temperatures and recycling of the ionic liquid and salt reduce the environmental impact of the algae processing while providing biofuels and other useful products.
대표청구항▼
1. A method for processing algae cells comprising the steps of: a) mixing an amount of wet algae cells with an amount of hydrophilic ionic liquid and thereby lysing said algae cells to produce a cell lysate, said lysate comprising at least 50% by weight ionic liquid;b) mixing an amount of salt with
1. A method for processing algae cells comprising the steps of: a) mixing an amount of wet algae cells with an amount of hydrophilic ionic liquid and thereby lysing said algae cells to produce a cell lysate, said lysate comprising at least 50% by weight ionic liquid;b) mixing an amount of salt with the cell lysate to form a salting-out suspension;c) allowing the salting-out suspension to rest for a time sufficient for the formation of a lipid-containing hydrophobic phase, an ionic liquid phase, and an aqueous salt solution phase; andd) isolating the lipid-containing hydrophobic phase, the ionic liquid phase, and the aqueous salt solution phase from one another to produce an algae lipid extract, an ionic liquid, and a dilute salt solution. 2. The method of claim 1, wherein a precipitate is formed during one or more of steps a), b), and c) and further comprising isolating the precipitate. 3. The method of claim 2, wherein steps b) and c) are repeated at least once. 4. The method of claim 1, and further comprising the step of reusing the ionic liquid phase in a subsequent mixing step a). 5. The method of claim 4, and further comprising the step of drying the ionic liquid phase before its reuse in the subsequent mixing step a). 6. The method of claim 1 and further comprising the steps of removing water from the aqueous salt solution phase to form a concentrated salt solution and using said concentrated salt solution in a subsequent mixing step b). 7. The method of claim 6 and further comprising the step of performing electrodialysis on the aqueous salt solution phase and/or the concentrated salt solution. 8. The method of claim 1, wherein the algae cells are in the form of intact, chopped, shredded, or ground marcoalgae. 9. The method of claim 1, wherein the algae cells are in the form of a water suspension of microalgae that contains approximately 0.1 kg to 1.0 kg algae cells per liter. 10. The method of claim 1, and further comprising the step of removing water from a culture of algae cells before mixing step a) so that the algae cells are present in a cell suspension in an amount of approximately 0.1 kg to 1.0 kg algae cells per liter of cell suspension. 11. The method of claim 1, wherein the hydrophilic ionic liquid is selected from the group consisting of 1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride, 1-Methyl-3-octylimidazolium chloride, 1-Hexyl-3-methylimidazolium chloride, 1-Hexyl-3-methylimidazolium iodide and mixtures thereof. 12. The method of claim 1, wherein mixing step a) is performed at a temperature of 100° C. or lower. 13. The method of claim 1, wherein mixing step a) is performed at a temperature of 70° C. or lower. 14. The method of claim 1, wherein mixing step a) is performed at a temperature of 40° C. or lower. 15. The method of claim 1, wherein the ionic liquid and wet algae cells are present in step a) in a mass ratio of between 20:1 and 2:1. 16. The method of claim 15, wherein the temperature of lysis in mixing step a) is between 70° C. and 105° C. 17. The method of claim 15, wherein the temperature of lysis in mixing step a) is between 20° C. and 70° C. 18. The method of claim 15, wherein the wet algae cells are present in a concentration of at least 80% by weight. 19. The method of claim 1, wherein the algae cells are selected from the group consisting of Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidea, Chlamydomonas reinhardtii, Chlamydomonas moewusii, Scenedesmus dimorphus, Selenastrum capricornotum, Chlorococcum (various species), Nannochloropsis oculata, Tetraselmis (various species), Sargassum muticum, Laminaria digitata, and mixtures thereof. 20. The method of claim 1, wherein the salt is in the form of a solid and/or an aqueous salt solution containing a salt selected from the group consisting of K3PO4, K2HPO4, K2SO4, MgS04, Li2S04, ZnS04, Al2(S04)3, Mg3(P04)2, Li3P04, Zn3(P04)2, ALP04, Na2CO3, (NH4)2S04, (NH4)2HPO4, and mixtures thereof.
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이 특허에 인용된 특허 (2)
Holzwarth,Thomas R.; Samuel,Bashir, Method of treatment of effluent stream.
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