Yersinia pestis antigens, vaccine compositions, and related methods
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/102
A61K-039/02
C07K-014/24
출원번호
US-0668258
(2008-07-11)
등록번호
US-8404252
(2013-03-26)
국제출원번호
PCT/US2008/069860
(2008-07-11)
§371/§102 date
20101022
(20101022)
국제공개번호
WO2009/009759
(2009-01-15)
발명자
/ 주소
Yusibov, Vidadi
Mett, Vadim
Musiychuk, Konstantin
출원인 / 주소
Fraunhofer USA, Inc.
대리인 / 주소
Fish & Richardson P.C.
인용정보
피인용 횟수 :
5인용 특허 :
74
초록▼
The present invention provides antigens and vaccines useful in prevention of infection by Yersinia pestis. The present invention provides pharmaceutical compositions of such antigens and/or vaccines. The present invention provides methods for the production of Y. pestis protein antigens in plants, a
The present invention provides antigens and vaccines useful in prevention of infection by Yersinia pestis. The present invention provides pharmaceutical compositions of such antigens and/or vaccines. The present invention provides methods for the production of Y. pestis protein antigens in plants, as well as methods for their use in the treatment and/or prevention of Y. pestis infection.
대표청구항▼
1. An isolated antigen comprising a Yersinia pestis protein fused to a modified thermostable protein; wherein the Yersinia pestis protein comprises a full-length F1 protein that is at least 95% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7, and wherein the modified thermostable pro
1. An isolated antigen comprising a Yersinia pestis protein fused to a modified thermostable protein; wherein the Yersinia pestis protein comprises a full-length F1 protein that is at least 95% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7, and wherein the modified thermostable protein is a LicKM protein. 2. The isolated antigen of claim 1, wherein the Yersinia pestis protein comprises an amino acid sequence that is at least 98% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7. 3. The isolated antigen of claim 1, wherein the Yersinia pestis protein comprises the amino acid sequence set forth as SEQ ID NO.: 1, 3, 5, or 7. 4. The isolated antigen of claim 1, wherein the Yersinia pestis protein fused to the modified thermostable protein is one or more of an N-terminal fusion, a C-terminal fusion, or a surface loop insertion fusion protein. 5. A vaccine composition comprising a pharmaceutically acceptable carrier and an antigen comprising a Yersinia pestis protein fused to a modified thermostable protein, wherein the Yersinia pestis protein comprises a full-length F1 protein that is at least 95% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7; wherein the modified thermostable protein is a LicKM protein; and wherein the composition is capable of eliciting an immune response upon administration to a subject. 6. The vaccine composition of claim 5, wherein the Yersinia pestis protein comprises an amino acid sequence that is at least 98% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7. 7. The vaccine composition of claim 5, wherein the Yersinia pestis protein comprises the amino acid sequence set forth as SEQ ID NO.: 1, 3, 5, or 7. 8. The vaccine composition of claim 5, wherein the Yersinia pestis protein fused to the modified thermostable protein is one or more of an N-terminal fusion, a C-terminal fusion, or a surface loop insertion fusion protein. 9. The vaccine composition of claim 5, wherein the antigen is produced in a plant selected from a transgenic plant and a plant transiently expressing the antigen. 10. The vaccine composition of claim 5, wherein the composition comprises antigen which is purified, partially purified, or unpurified from plant cells, a plant, seeds, fruit, or an extract thereof. 11. The vaccine composition of claim 5, further comprising at least one vaccine adjuvant. 12. The vaccine composition of claim 11, wherein the adjuvant is selected from the group consisting of complete Freund's adjuvant, incomplete Freund's adjuvant, alum, MF59, saponin, aluminum hydroxide, QuilA, and macrophage-activating lipopeptide (Malp-2). 13. A method for inducing a protective immune response against Yersinia pestis infection in a subject comprising administering to a subject an effective amount of an anti-Yersinia pestis vaccine composition, wherein the administration is sufficient to stimulate production of antigen specific antibodies or stimulate a cellular immune response by the subject; thereby inducing a protective immune response; wherein the vaccine composition comprises an antigen comprising a Yersinia pestis protein fused to a modified thermostable protein; and wherein the Yersinia pestis protein comprises a full-length F1 protein that is at least 95% identical to the sequence set forth as SEQ ID NO:1, 3, 5, or 7; and wherein the modified thermostable protein is a LicKM protein. 14. The method of claim 13, wherein the composition is administered orally, intranasally, subcutaneously, intravenously, intraperitoneally, or intramuscularly. 15. The method of claim 14, wherein the composition is administered orally via feeding plant cells to the subject. 16. The method of claim 13, wherein the subject is human. 17. The method of claim 13, wherein the Yersinia pestis protein comprises an amino acid sequence that is at least 98% identical to the sequence set forth as SEQ ID NO: 1, 3, 5, or 7. 18. The method of claim 13, wherein the Yersinia pestis protein comprises the amino acid sequence set forth as SEQ ID NO: 1, 3, 5, or 7.
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