Provided are electrokinetically-altered fluids (e.g., gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cel
Provided are electrokinetically-altered fluids (e.g., gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellular membrane potential and cellular membrane conductivity. Further provided are the methods of making the electrokinetically-altered ionic aqueous fluid compositions. Particular aspects provide for regulating or modulating intracellular signal transduction associated by modulation of at least one of cellular membranes, membrane potential, membrane proteins such as membrane receptors, including but not limited to G-Protein Coupled Receptors (GPCR), and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes). Other embodiments include particular methods of producing the electrokinetically-altered fluids. The electrokinetically-altered fluid compositions and methods of producing the fluid include electrokinetically-altered ionic aqueous fluids optionally in the form of solvated electrons stabilized with molecular oxygen.
대표청구항▼
1. A electrokinetically-altered fluid composition, comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous solution in an amount sufficient to provide, upon cont
1. A electrokinetically-altered fluid composition, comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous solution in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity. 2. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized oxygen-containing nanostructures comprise charge-stabilized oxygen gas-containing nanostructures. 3. The electrokinetically-altered fluid composition of claim 1, wherein the percentage of dissolved oxygen molecules present in the fluid as the charge-stabilized oxygen-containing nanostructures is a percentage greater than 0.01%. 4. The electrokinetically-altered fluid composition of claim 1, wherein the percentage of dissolved oxygen molecules present in the fluid as the charge-stabilized oxygen-containing nanostructures is a percentage greater than 50%. 5. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized oxygen-containing nanostructures have an average diameter of less than 90 nm. 6. The electrokinetically-altered fluid composition of claim 1, wherein the ionic aqueous solution comprises a saline solution. 7. The electrokinetically-altered fluid composition of claim 1, wherein the fluid is superoxygenated. 8. The electrokinetically-altered fluid composition of claim 1, wherein the fluid comprises at least one of a form of solvated electrons, and an electrokinetically modified or charged oxygen species. 9. The electrokinetically-altered fluid composition of claim 8, wherein the form of solvated electrons or electrokinetically modified or charged oxygen species are present in an amount of at least 0.01 ppm. 10. The electrokinetically-altered fluid composition of claim 8, wherein the electrokinetically-altered fluid comprises a form of solvated electrons stabilized, at least in part, by molecular oxygen. 11. The electrokinetically-altered fluid composition of claim 1, wherein the ability to modulation of at least one of cellular membrane potential and cellular membrane conductivity persists for at least two months in a closed gas-tight container. 12. The electrokinetically-altered fluid composition of claim 1, wherein alteration of the electrokinetically altered aqueous fluid comprises exposure of the fluid to hydrodynamically-induced, localized electrokinetic effects. 13. The electrokinetically-altered fluid composition of claim 12, wherein, exposure to the localized electrokinetic effects comprises exposure to at least one of voltage pulses and current pulses. 14. The electrokinetically-altered fluid composition of claim 12, wherein the exposure of the fluid to hydrodynamically-induced, localized electrokinetic effects, comprises exposure of the fluid to electrokinetic effect-inducing structural features of a device used to generate the fluid. 15. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises altering of a conformation, ligand binding activity, or a catalytic activity of a membrane associated protein. 16. The electrokinetically-altered fluid composition of claim 15, wherein the membrane associated protein comprises at least one selected from the group consisting of receptors, transmembrane receptors, ion channel proteins, intracellular attachment proteins, cellular adhesion proteins, and integrins. 17. The electrokinetically-altered fluid composition of claim 16, wherein the transmembrane receptor comprises a G-Protein Coupled Receptor (GPCR). 18. The electrokinetically-altered fluid composition of claim 17, wherein the G-Protein Coupled Receptor (GPCR) interacts with a G protein α subunit. 19. The electrokinetically-altered fluid composition of claim 18, wherein the G protein α subunit comprises at least one selected from the group consisting of Gαs, Gαi; Gαq, and Gα12. 20. The electrokinetically-altered fluid composition of claim 19, wherein the at least one G protein α subunit is Gαq. 21. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity, comprises modulating whole-cell conductance. 22. The electrokinetically-altered fluid composition of claim 21, wherein modulating whole-cell conductance, comprises modulating at least one of a linear and a non-linear voltage-dependent contribution of the whole-cell conductance. 23. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of a calcium dependant cellular messaging pathway or system. 24. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of phospholipase C activity. 25. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of adenylate cyclase (AC) activity. 26. The electrokinetically-altered fluid composition of claim 1, wherein the electrokinetically-altered aqueous fluid comprises dissolved oxygen in an amount of at least 8 ppm at atmospheric pressure. 27. The electrokinetically-altered fluid composition of claim 26, wherein the oxygen in the fluid or solution is present in an amount of at least 25 ppm. 28. A method of producing an electrokinetically-altered aqueous fluid or solution, comprising: providing a flow of a fluid material between two spaced surfaces in relative motion and defining a mixing volume therebetween, wherein the dwell time of a single pass of the flowing fluid material within and through the mixing volume is greater than 0.06 seconds or greater than 0.1 seconds; andintroducing oxygen (O2) into the flowing fluid material within the mixing volume under conditions suitable to dissolve at least 20 ppm oxygen into the material, and electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 29. The method of claim 28, wherein the oxygen is infused into the material in less than 400 milliseconds. 30. An electrokinetically altered oxygenated aqueous fluid or solution made according to any one of claims 28 and 29. 31. A method of producing an electrokinetically-altered aqueous fluid or solution, comprising: providing a flow of a fluid material between two spaced surfaces defining a mixing volume therebetween; andintroducing oxygen into the flowing material within the mixing volume under conditions suitable to infuse at least 20 ppm oxygen into the material in less than 100 milliseconds, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 32. The method of claim 31, wherein the dwell time of the flowing material within the mixing volume is greater than 0.06 seconds. 33. The method of any one of claims 28 through 31, wherein the ratio of surface area to the volume is at least 12. 34. An electrokinetically altered oxygenated aqueous fluid or solution made according to any one of claims 31 and 32. 35. A method of producing an electrokinetically-altered aqueous fluid or solution, comprising use of a mixing device for creating an output mixture by mixing a first material and a second material, the device comprising: a first chamber configured to receive the first material from a source of the first material;a stator;a rotor having an axis of rotation, the rotor being disposed inside the stator and configured to rotate about the axis of rotation therein, at least one of the rotor and stator having a plurality of through-holes;a mixing chamber defined between the rotor and the stator, the mixing chamber being in fluid communication with the first chamber and configured to receive the first material therefrom, and the second material being provided to the mixing chamber via the plurality of through-holes formed in the one of the rotor and stator;a second chamber in fluid communication with the mixing chamber and configured to receive the output material therefrom; anda first internal pump housed inside the first chamber, the first internal pump being configured to pump the first material from the first chamber into the mixing chamber, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 36. A method of producing an electrokinetically altered oxygenated aqueous fluid or solution, comprising use of a mixing device for creating an output mixture by mixing a first material and a second material, the device comprising: a stator;a rotor having an axis of rotation, the rotor being disposed inside the stator and configured to rotate about the axis of rotation therein;a mixing chamber defined between the rotor and the stator, the mixing chamber having an open first end through which the first material enters the mixing chamber and an open second end through which the output material exits the mixing chamber, the second material entering the mixing chamber through at least one of the rotor and the stator;a first chamber in communication with at least a majority portion of the open first end of the mixing chamber; anda second chamber in communication with the open second end of the mixing chamber, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures having an average diameter of less than about 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 37. The method of claim 35, wherein the first internal pump is configured to impart a circumferential velocity into the first material before it enters the mixing chamber. 38. The electrokinetically-altered fluid composition of claim 1, wherein the amount of oxygen present in charge-stabilized oxygen-containing nanostructures of the electrokinetically-altered fluid is at least 8 ppm at atmospheric pressure. 39. The composition of claim 1, wherein the charge-stabilized oxygen-containing nanostructures comprise at least one of nanocages, nanoclusters and nanobubbles. 40. The composition of claim 39, comprising saline or oxygen enriched saline.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (322)
Wallace, Scott D., Absorption field reclamation and maintenance system.
Borroni, Edilio Maurizio; Huber-Trottmann, Gerda; Kilpatrick, Gavin John; Norcross, Roger David, Adenosine receptor ligands and their use in the treatment of disease.
Varlamov Vladimir M. (ulitsa 50 let Oktyabrya ; 1 ; kv. 16 Zlatoust Chelyabinskoi oblasti SUX) Sopin Anatoly I. (prospekt Gagarina ; 4 linia ; 2 ; kv. 7 Zlatoust Chelyabinskoi oblasti SUX) Judaev Vas, Apparatus for creating acoustic oscillations in a running liquid medium.
Carre Olof G. (AlnSEX) Josefsson Paul W. (Srberge SEX) Nsman Lars E. (AlnSEX) Zetterqvist Stig B. H. (Sundsbruk SEX), Apparatus for gas or liquid admixture.
Dahllf Hakan (Edsvalla SEX) Ekholm Rolf (Karlstad SEX) Jansson Ulf (Karlstad SEX), Apparatus for mixing a suspension of cellulosic fibrous material and fluid.
Vincent Divino, Jr. ; Seth A. Foerster ; James M. Gessert ; Robert A. Mest ; Paul J. Zalesky, Apparatus for the preparation and delivery of gas-enriched fluids.
Jubin Robert T. (Powell TN) Randolph John D. (Maryville TN), Centrifugal contactor with liquid mixing and flow control vanes and method of mixing liquids of different phases.
Hideyuki Mori JP; Atsushi Komatsu JP; Makoto Kokubun JP; Toyohiko Yamadera JP; Mitsuo Hamada JP, Continuous mixing apparatus with upper and lower disk impellers each having scrapers.
Sher Samuel E. (Rockaway NJ) Borgianini Stephen A. (Mount Holly NJ) Carpenter Robert E. (Nutley NJ) Santora Scott (Hammonton NJ) Scavuzzo William S. (Clark NJ), Device for taking, preserving and transporting a fluid sample for analysis.
Mayer Karl M. (Immenhofen 55 8951 Ruderatshofen DEX) Schfer Rudolf (Blaichacher Str. 21 8971 Burgberg DEX), Device for the continuous mixing of a dry finished mortar.
Sumita,Osao, Electrolytic cell for producing charged anode water suitable for surface cleaning or treatment, and method for producing the same and use of the same.
Miyashita Kohichi,JPX ; Nagano Keiji,JPX ; Nakamura Toshikazu,JPX, Electrolyzed functional water, and production process and production apparatus thereof.
Nemes Sandor (Tatabanya HUX) Solymos Karoly (Tatabanya HUX) Barna Laszlo (Budapest HUX) Bognar Tibor (Budapest HUX) Szigetvari Jozsef (Budapest HUX) Vajda Ferenc (Budapest HUX) Tornai Endre (Budapest, Equipment for the delivery of slurries and for refinement during delivery.
Gouttebessis Jacques (Volvic FRX) Martel Michel (Clermont-Ferrand FRX), Installation for preparing a liquid or pasty mixture intended to be molded and method for the use of said installation.
Kohlmetz Charles W. (27 Brook Hill La. Rochester NY 14625) Urban James J. (Main St. Dunstable MA 01827) Kenny John (Main St. Dunstable MA 01827), Kinetic heater.
Ashbrook Clifford L. (Rte. 2 ; Box 439 Spicewood TX 78669) Scarborough Douglas B. (Rte. 27 ; Box 124-A-3 San Antonio TX 78238), Magnetic molecular agglomerate reducer and method.
Kuo Alex C. (Charleston WV) Nielsen Kenneth A. (Charleston WV) Condron James A. (Hurricane WV) Hoy Kenneth L. (St. Albans WV), Method and apparatus for metering and mixing non-compressible and compressible fluids.
Kuo Alex C. (Charleston WV) Nielsen Kenneth A. (Charleston WV) Condron James A. (Hurricane WV) Hoy Kenneth L. (St. Albans WV), Method and apparatus for metering and mixing non-compressible and compressible fluids.
Nyman Bror G. (Ulvila FIX) Hultholm Stig-Erik (Pori FIX) Lilja Launo L. (Pori FIX) Mkitalo Valto J. (Pori FIX), Method and apparatus for mixing and separating two liquid phases while preventing aeration and emulsions using a mixer-s.
Aanonsen Bent (Saetre NOX) Odberg Paul-Johny (Saetre NOX) Samuelsen Eirik (Lierbyen NOX), Method and apparatus for the continuous production of a slurry explosive containing an emulsified liquid component.
Huber Gordon R. ; Strahm Bradley L. ; Sevatson Eric S. ; Kesler David L., Method and apparatus for the production of high viscosity paste products with added components.
Ashbrook Clifford L. (Rte. 2 ; Box 439 Spicewood TX 78669) Scarborough Douglas B. (Rte. 17 ; Box 124-A3 San Antonio TX 78238), Method and apparatus for treating cooling tower water.
Divino ; Jr. Vincent ; Foerster Seth A. ; Gessert James M. ; Mest Robert A. ; Zalesky Paul J. ; Spears James Richard, Method for the preparation and delivery of gas-enriched fluids.
Divino, Jr., Vincent; Foerster, Seth A.; Gessert, James M.; Mest, Robert A.; Zalesky, Paul J.; Spears, James Richard, Method for the preparation and delivery of gas-enriched fluids.
Lambert Karel J. (San Diego CA) Jablonski Edward G. (Escondido CA) Hulle Carl (San Diego CA), Method of making encapsulated gas microspheres useful as an ultrasonic imaging agent.
Wood, Anthony B.; Archambeau, Gregory J.; Watson, Richard L., Mixing device for creating an output mixture by mixing a first material and a second material.
Iwako Hiroyuki (Tokyo JPX) Ando Shigeki (Tokyo JPX), Process and apparatus for mechanically mixing two immiscible liquids and one or more other substances.
Snyder Larry L. (Lincoln NE) Smith ; Jr. Martin T. (Lincoln NE), Spraying apparatus having a fluid storage tank with agitator and anti-vortex tank fittings.
Derek, Daw J.; Patterson, William R.; Myrick, Stephen E.; Creech, Jeffrey L.; Divino, Jr., Vincent; Watson, Gregory P.; Zalesky, Paul J., System for enriching a bodily fluid with a gas.
Mano, Yoshihiro; Sato, Kenji; Cho, Yuichiro; Chiba, Kaneo, Tissue preservation method comprising contacting tissue with a solution of nanobubbles and salt.
Erickson Lennart G. (1070 E. Meadow Cir. Palo Alto CA 94303) Worne Howard E. (Lyon Industrial Pk. ; Rte. 73 Berlin NJ 08009), Wastewater energy recycling method.
Wood, Anthony B.; Archambeau, Gregory J.; Watson, Richard L., Mixing device for creating an output mixture by mixing a first material and a second material.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.