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A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel

A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.

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1. A degas assembly comprising: a low pressure fluid channel for carrying a wash fluid at a first pressure;a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure;a degas separator defining a fluid barrier between the low pressure fluid channel a

1. A degas assembly comprising: a low pressure fluid channel for carrying a wash fluid at a first pressure;a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure;a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel;wherein the pressurized fluid channel extends along an outer periphery of the low pressure fluid channel, and the second pressure is at least about 1000 psi. 2. The degas assembly according to claim 1, the degas assembly comprises a central lumen extending within an outer tubing, wherein the central lumen forms the low pressure fluid channel, an annular space between the central lumen and the tubing defines the pressurized channel, and a wall of the central lumen defines the degas separator. 3. The degas assembly according to claim 1, wherein the pressurized fluid channel extends along substantially the entire outer periphery and substantially the entire length of the low pressure fluid channel. 4. The degas assembly according to claim 1, wherein the eluent is received from an eluent generator and has a pressure of at least about 3300 psi. 5. The degas assembly according to claim 1, wherein the eluent is received from an eluent generator and has a pressure between about 3300 psi and about 5000 psi. 6. The degas assembly according to claim 1, further comprising a low pressure channel member defining the low pressure fluid channel and a pressurized channel member defining the pressurized fluid channel, each of the channel members comprising inert polymer tubing. 7. The degas assembly according to claim 6, wherein the low pressure fluid channel member comprises amorphous fluoropolymer tubing. 8. The degas assembly according to claim 6, wherein the pressurized fluid channel member comprises reinforced polyetheretherketone (PEEK) tubing. 9. The degas assembly according to claim 1, further comprising: an inlet housing including: an eluent inlet for connecting to an eluent generator; anda wash inlet for connecting to a wash source; andan outlet housing including: an eluent outlet; anda wash outlet. 10. The degas assembly according to claim 9, wherein the low pressure fluid channel is fluidicly connected to the wash inlet and the wash outlet, and the pressurized fluid channel is fluidicly connected to the eluent inlet and the eluent outlet. 11. The degas assembly according to claim 10, the low pressure fluid channel and pressurized fluid channel being substantially coaxial flexible tubes, wherein at least one of the low pressure and pressurized tubes includes a splined portion at an outlet end and clamped within the outlet housing, the splined portion configured to allow fluid to flow through the splines when the splined portion is clamped. 12. The degas assembly according to claim 11, wherein the splined portion extends along substantially the entire length of the pressurized tube. 13. The degas assembly according to claim 2, wherein the outer tubing has a non-circular cross-section. 14. The degas assembly according to claim 2, wherein an outer surface of the central lumen is non-cylindrical. 15. A liquid chromatography system comprising: the degas assembly according to claim 10; anda pressurized liquid chromatography column;wherein eluent from the outlet housing flows to the column. 16. The system according to claim 15, further comprising a pump, wherein the degas assembly is positioned downstream from the pump. 17. The system according to claim 15, further comprising a pump, wherein the degas assembly is positioned upstream from an inlet of the pump. 18. A method of separating gas from an eluent for liquid chromatography, the method comprising: flowing high pressure eluent to a degas assembly, the degas assembly including an inner flow channel and outer flow channel with a degas separator disposed therebetween, said degas separator being a permeable membrane;flowing the eluent to the outer flow channel; andseparating gas from the eluent into the inner flow channel hut retaining liquid from the eluent in the outer flow channel via the degas separator;wherein the high pressure eluent has a pressure of at least about 1000 psi. 19. The method according to claim 18, wherein the eluent includes gas resulting from electrolysis. 20. The method according to claim 18, further comprising directing the separated eluent from an outlet of the outer flow channel to a liquid chromatography column. 21. The method according to claim 18, further comprising flowing regenerant through the inner flow channel thereby flushing the separated gas. 22. The method according to claim 18, wherein the eluent is received from an eluent generator. 23. The method according to claim 22, wherein eluent from the eluent generator is at a pressure of at least about 3300 psi. 24. The method according to claim 22, wherein eluent from the eluent generator is at a pressure of between about 3300 psi and about 5000 psi. 25. The method according to claim 18, wherein the degas separator is a polymer tube. 26. The method according to claim 18, the outer flow channel at least partially enveloping the inner flow channel, wherein the flowing eluent to the outer flow channel is performed so as to place the inner flow channel in compression. 27. The degas assembly according to claim 1, the pressurized channel being in fluid communication with an eluent generator and at least one of a chromatography column and a suppressor, wherein the pressurized channel is located upstream from the at least one of a chromatography column and a suppressor. 28. The degas assembly according to claim 6, wherein the low pressure channel member includes at least one flared end, and the at least one flared end is configured to form a fluid seal when engaged with a fluid inlet or outlet port. 29. The degas assembly according to claim 28, wherein the at least one flared end is thermally formed. 30. The degas assembly according to claim 1, wherein the low pressure fluid channel is capped at one end. 31. A degas assembly comprising: a low pressure fluid channel for carrying a wash fluid at a first pressure;a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, the pressurized channel including an eluent inlet downstream from an eluent generator, and an eluent outlet located upstream from a sample injector; anda degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, wherein the pressurized fluid channel extends along an outer periphery of the low pressure fluid channel;wherein the degas separator retains liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel in order to remove gas from the eluent before the eluent reaches the sample injector.