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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0568887 (2009-09-29) |
등록번호 | US-8425758 (2013-04-23) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 0 인용 특허 : 455 |
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agen
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
1. A method for determining a concentration of an analyte in a sample fluid, comprising the steps of: positioning a sensor in a sensor reader;contacting a sample fluid with a sensor, wherein the sensor comprises: a working electrode and a counter electrode;a first substrate having a proximal end and
1. A method for determining a concentration of an analyte in a sample fluid, comprising the steps of: positioning a sensor in a sensor reader;contacting a sample fluid with a sensor, wherein the sensor comprises: a working electrode and a counter electrode;a first substrate having a proximal end and a distal end, the first substrate defining a first side edge and a second side edge of the electrochemical sensor extending from the proximal end to the distal end of the first substrate, the distal end being configured and arranged for insertion into a sensor reader;a second substrate disposed over the first substrate;a spacer comprising a first part and a second part disposed between the first and second substrates, wherein the first part of the spacer, the second part of the spacer, the first substrate, and the second substrate define a first aperture along the first side edge of the sensor and a second aperture along the second side edge of the sensor;a sample chamber for holding the sample fluid, the sample chamber comprising the working electrode, the counter electrode, an analyte-responsive enzyme and a redox mediator, wherein the sample chamber is sized to contain a volume of no more than about 1 μL of the sample fluid, and wherein the sample chamber has a length which extends from the first aperture to the second aperture and a substantially uniform width between the first part of the spacer and the second part of the spacer along the entire length of the sample chamber, and wherein the width of the sample chamber is not wider than either the first aperture or the second aperture;wherein following contacting the sample fluid with the sensor, the sensor reader: activates an auditory or a visual sign to alert a user when the sample chamber is sufficiently filled for initiating a reading;initiates a reading by generating a sensor signal at the working electrode, anddetermines the concentration of the analyte using the sensor signal. 2. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by coulometry using the sensor signal. 3. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by amperometry using the sensor signal. 4. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by potentiometry using the sensor signal. 5. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by chronoamperometry using the sensor signal. 6. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by chronopotentiometry using the sensor signal. 7. The method according to claim 1, wherein determining the concentration of the analyte comprises determining the concentration of the analyte by a Cotrell measurement technique using the sensor signal. 8. The method according to claim 1, further comprising: providing calibration data on a batch of the electrochemical sensors to a measurement instrument, said calibration data comprising information related to a magnitude of a background charge for the batch of the electrochemical sensors;wherein the step of determining the concentration of the analyte comprises determining the concentration of the analyte using the sensor signal and the calibration data. 9. The method according to claim 1, wherein the sensor reader activates a visual sign to alert a user when the sample chamber is sufficiently filled for initiating a reading. 10. The method according to claim 1, wherein the sensor reader activates an auditory sign to alert a user when the sample chamber is sufficiently filled for initiating a reading. 11. The method according to claim 1, wherein the analyte is glucose or a ketone. 12. The method according to claim 11, wherein the analyte is glucose and the analyte-responsive enzyme is glucose dehydrogenase or glucose oxidase. 13. The method according to claim 12, wherein the analyte-responsive enzyme is pyrroloquinoline quinone (PQQ) glucose dehydrogenase. 14. The method according to claim 1, wherein the redox mediator comprises a transition metal complex. 15. The method according to claim 14, wherein the transition metal complex comprises a transition metal selected from the group consisting of osmium, ruthenium, iron and cobalt. 16. The method according to claim 15, wherein the transition metal is osmium. 17. The method according to claim 14, wherein the transition metal complex comprises two or more ligands coordinately bound to a transition metal, wherein at least one of the ligands is a heterocyclic nitrogen-containing bidentate ligand. 18. The method according to claim 17, wherein the transition metal is osmium. 19. The method according to claim 1, wherein the redox mediator comprises ferricyanide. 20. The method according to claim 1, wherein the sensor comprises a proximal end having a width and a distal end having a width, the distal end being configured for insertion into a sensor reader, wherein the width of the distal end is greater than the width of the proximal end. 21. The method according to claim 1, wherein the working electrode is disposed on a first substrate and the counter electrode is disposed on a second substrate. 22. The method according to claim 21, wherein the sensor comprises a proximal end and a distal end, the distal end being configured for insertion into a sensor reader, wherein the first substrate comprises a cutout portion at the distal end to expose a portion of the counter electrode disposed on the second substrate. 23. The method according to claim 21, wherein sensor comprises a proximal end and a distal end, the distal end being configured for insertion into a sensor reader, wherein the second substrate comprises a cutout portion at the distal end to expose a portion of the working electrode disposed on the first substrate. 24. The method according to claim 1, wherein the working electrode comprises a material selected from the group consisting of gold, carbon, platinum, ruthenium dioxide, and palladium. 25. The method according to claim 1, wherein the counter electrode comprises a material selected from the group consisting of gold, silver, silver chloride, silver bromide, carbon, platinum, ruthenium dioxide, and palladium. 26. The method according to claim 1, comprising obtaining the sample fluid from a user. 27. The method according to claim 26, wherein the sample fluid is blood obtained from a finger of the user. 28. The method according to claim 26, wherein the sample fluid is blood obtained from a region of the user having a lower nerve end density as compared to a fingertip. 29. The method according to claim 28, wherein the region of the user having a lower nerve end density as compared to a fingertip is selected from the group consisting of: a forearm region, and a thigh region.
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