초록 ▼

A semen extender composition is provided. The semen extender composition includes a sperm cell protecting amount of phospholipid, an effective amount of surfactant to reduce ice crystal formation during freezing of the composition; a carbohydrate, and a biological buffer to provide a semen extender

A semen extender composition is provided. The semen extender composition includes a sperm cell protecting amount of phospholipid, an effective amount of surfactant to reduce ice crystal formation during freezing of the composition; a carbohydrate, and a biological buffer to provide a semen extender use solution having a pH of between about 6.9 and about 7.5, and wherein the use solution exhibits an osmolality of about 250 mOsM to about 350 mOsM. Methods for manufacturing and using a semen extender composition are provided.

대표청구항 ▼

1. A method for freezing semen comprising: (a) introducing ejaculate comprising sperms cells into a semen extender composition to provide a cryogenic solution, the semen extender composition comprising:(i) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source;(ii) about 0.0

1. A method for freezing semen comprising: (a) introducing ejaculate comprising sperms cells into a semen extender composition to provide a cryogenic solution, the semen extender composition comprising:(i) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source;(ii) about 0.0001 wt. % to about 1 wt. % anionic surfactant comprising a sulfate;(iii) about 0.5 wt. % to about 3 wt. % carbohydrate;(iv) about 3 wt. % to about 14 wt. % freeze agent; and(v) biological buffer to provide the composition with a pH of about 6.9 to about 7.5, and wherein the composition comprises a sufficient amount of water so that the composition exhibits an osmolality of about 250 mOsM to about 350 mOsM, wherein the composition is substantially free of animal products; and(b) freezing the cryogenic solution to provide a frozen solution. 2. A method according to claim 1, further comprising a step of: (a) thawing the frozen solution to provided a thawed solution. 3. A method according to claim 2, further comprising a step of: (a) washing the sperm cells recovered from the thawed solution. 4. A method according to claim 1, where in the step of introducing an ejaculate into a semen extender composition comprises buffering raw ejaculate to provide a buffered ejaculate, a combining the buffered ejaculate with the semen extender composition. 5. A method according to claim 1, wherein the composition comprises at least about 90 wt. % water. 6. A method according to claim 1, further comprising: (a) antioxidant. 7. A method according to claim 4, wherein the antioxidant comprises at least one of vitamin E, vitamin C, vitamin A, BHA, BHT, or derivatives thereof. 8. The method according to claim 1, wherein the anionic surfactant comprises at least one of sodium lauryl sulfate, sodium laureth sulfate, or mixtures thereof. 9. The method according to claim 1, wherein the semen extender composition further comprises at least about 1 IU/ml antioxidant. 10. The method according to claim 1, wherein the semen extender composition further comprises at least about 5 IU/ml antioxidant. 11. The method according to claim 1, wherein the semen extender composition further comprises about 1 wt. % to about 3 wt. % antioxidant. 12. The method according to claim 9, wherein the antioxidant comprises at least one of vitamin E, vitamin C, vitamin A, BHA, BHT, or derivatives thereof. 13. The method according to claim 1, wherein the freeze agent comprises polyol. 14. The method according to claim 1, wherein the freeze agent comprises dimethylsulfoxide. 15. The method according to claim 1, wherein the phospholipid comprises lecithin.