A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matr
A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
대표청구항▼
1. A tissue matrix comprising isolated CD34−, CD10+, SH2+ placental stem cells, wherein said tissue matrix is synthetic. 2. The tissue matrix of claim 1 wherein said placental stem cells are additionally CD90+ or CD45−. 3. The tissue matrix of claim 2 wherein said placental stem cells are additional
1. A tissue matrix comprising isolated CD34−, CD10+, SH2+ placental stem cells, wherein said tissue matrix is synthetic. 2. The tissue matrix of claim 1 wherein said placental stem cells are additionally CD90+ or CD45−. 3. The tissue matrix of claim 2 wherein said placental stem cells are additionally CD90+ and CD45−. 4. The tissue matrix of claim 1 wherein said placental stem cells are additionally one or more of CD29+, CD38−, CD44+, CD54+, SH3+ or SH4+. 5. The tissue matrix of claim 4 wherein said placental stem cells are additionally one or more of CD29+, CD38−, CD44+, CD54+, SH3+ or SH4+. 6. The tissue matrix of claim 4 wherein said placental stem cells are CD44+. 7. The tissue matrix of claim 5 wherein said placental stem cells are CD44+. 8. The tissue matrix of claim 1, wherein said placental stem cells are additionally MHC Class II−. 9. The tissue matrix of claim 1 wherein said placental stem cells are tissue culture plastic adherent. 10. The tissue matrix of claim 1, wherein said placental stem cells are additionally one or more of CD29+, CD45−, CD90+, SH3+, SH4+, or MHC Class II−. 11. The tissue matrix of claim 10, wherein said placental stem cells are additionally each of CD29+, CD45−, CD90+, SH3+, SH4+, and MHC Class II−. 12. A method of making a tissue matrix, comprising seeding isolated CD34−, CD 10+, SH2+ placental stem cells onto a synthetic tissue matrix, wherein said plcental stem cells are from a human placenta that has been drained of cord blood. 13. The method of claim 12 wherein said placental stem cells are additionally CD90+ or CD45−. 14. The method of claim 13 wherein said placental stem cells are additionally CD90+ and CD45−. 15. The method of claim 12 wherein said placental stem cells are additionally one or more of CD29+, CD38−, CD44+, CD54+, SH3+ or SH4+. 16. The method of claim 13 wherein said placental stem cells are additionally one or more of CD29+, CD38−, CD44+, CD54+, SH3+ or SH4+. 17. The method of claim 13 wherein said placental stem cells are CD44+. 18. The method of claim 14 wherein said placental stem cells are CD44+. 19. The method of claim 12, wherein said placental stem cells are additionally MHC Class II−. 20. The method of claim 12 wherein said placental stem cells are tissue culture plastic adherent. 21. The method of claim 12, wherein said placental stem cells are additionally one or more of CD29+, CD45−, CD90+, SH3+, SH4+, or MHC Class II−. 22. The method of claim 21, wherein said placental stem cells are additionally each of CD29+, CD45−, CD90+, SH3+, SH4+, and MHC Class II−.
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