최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0241353 (2008-09-30) |
등록번호 | US-8440429 (2013-05-14) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 0 인용 특허 : 338 |
An automated method for preparing and amplifying a sequence contained in a nucleic acid present in a sample, the nucleic acid being prepared in a receptacle that is part of a unit that includes a plurality of receptacles and holds a removable contact-limiting element for aspirating a fluid component
An automated method for preparing and amplifying a sequence contained in a nucleic acid present in a sample, the nucleic acid being prepared in a receptacle that is part of a unit that includes a plurality of receptacles and holds a removable contact-limiting element for aspirating a fluid component of the sample from the receptacle.
1. A method for detecting the presence of a nucleic acid in a sample, the method comprising performing in an analyzer the automated steps of: (a) combining, in a receptacle, a sample containing a nucleic acid and a solid support comprising a magnetically-responsive particle, the solid support formin
1. A method for detecting the presence of a nucleic acid in a sample, the method comprising performing in an analyzer the automated steps of: (a) combining, in a receptacle, a sample containing a nucleic acid and a solid support comprising a magnetically-responsive particle, the solid support forming a complex with the nucleic acid, wherein the complex is suspended in a fluid component of the sample, the receptacle being part of a unit comprising a plurality of integrally formed receptacles, and the unit holding a removable contamination-limiting element configured to permit passage of the fluid component of the sample therethrough;(b) subjecting the contents of the receptacle to a magnetic field, thereby isolating the nucleic acid within the receptacle;(c) mounting the contamination-limiting element onto a fluid aspirator;(d) purifying the nucleic acid isolated in step (b) by contacting the fluid component of the sample with the contamination-limiting element and aspirating at least a portion of the fluid component with the fluid aspirator while the complex remains subjected to the magnetic field;(e) forming a reaction mixture comprising the purified nucleic acid of step (d) and all reagents required to perform a nucleic acid amplification;(f) performing a nucleic acid amplification with the reaction mixture formed in step (e), the nucleic acid amplification resulting in the enzymatic synthesis of amplification products, each of the amplification products comprising (i) a target sequence contained in the nucleic acid or (ii) the complement of the target sequence, wherein the reaction mixture is modified by the synthesis of the amplification products;(g) selectively hybridizing a detectably labeled probe to an amplification product that is one of the amplification products synthesized in step (f), thereby forming a hybrid in the modified reaction mixture that comprises the probe and the amplification product, wherein the amplification product comprises the target sequence or its complement, and wherein the probe hybridizes to the target sequence or its complement in the amplification product; and(h) while the hybrid formed in step (g) is in the modified reaction mixture, detecting the presence of the hybrid, wherein the presence of the hybrid in the modified reaction mixture is an indication of the presence of the nucleic acid in the sample,wherein the method is performed without human intervention. 2. The method of claim 1, wherein each of the receptacles comprises a cylindrical tube. 3. The method of claim 1, further comprising performing in the analyzer an automated step of sensing the level of the fluid component in step (d), wherein the contamination-limiting element is mounted on an aspirator tube of the fluid aspirator, and wherein the aspirator tube and the contamination-limiting element constitute a portion of a capacitor for performing capacitive fluid level sensing. 4. The method of claim 1, wherein the contamination-limiting element is one of a plurality of contamination-limiting elements held by the unit, wherein each of the contamination-limiting elements is configured to permit passage of a fluid component of a sample therethrough, and wherein each of the contamination-limiting elements is operationally associated with one of the receptacles of the unit. 5. The method of claim 1, wherein the contamination-limiting element is held in a frictional fit by the unit when the contamination-limiting element is mounted onto the fluid aspirator in step (c). 6. The method of claim 1, wherein the complex further comprises a capture probe, and wherein the capture probe is immobilized on the solid support and hybridized to the nucleic acid. 7. The method of claim 1, further comprising performing in the analyzer an automated step of washing the solid support one or more times with a wash buffer after step (d) and prior to step (e). 8. The method of claim 1, wherein each of the amplification products consists of a nucleic acid sequence having the target sequence or its complement. 9. The method of claim 1, wherein the amplification products include nucleic acid sequences having a sequence that is the same as the target sequence and nucleic acid sequences having a sequence that is the complement of the target sequence. 10. The method of claim 1, wherein step (f) is carried out in a temperature-controlled incubator maintained at a substantially uniform temperature. 11. The method of claim 1, further comprising performing in the analyzer, prior to step (h) and after step (g), an automated step of providing a selection reagent to the modified reaction mixture, whereby a label of an unhybridized, detectably labeled probe in the modified reaction mixture is selectively hydrolyzed, wherein the label is an acridinium ester. 12. The method of claim 1, wherein step (f) is performed in a temperature-controlled chamber of a first incubator, and wherein step (g) is performed in a temperature-controlled chamber of a second incubator, the first and second incubators being in a spaced-apart relationship. 13. The method of claim 1, wherein the hybrid is in solution in steps (g) and (h). 14. The method of claim 1, wherein the steps of the method are performed within a housing of the analyzer. 15. The method of claim 14, wherein the steps of the method are performed at multiple locations of a processing deck contained within the housing. 16. The method of claim 14, wherein the housing remains closed during the method. 17. A method for detecting the presence of a nucleic acid in a sample, the method comprising performing in an analyzer the automated steps of: (a) combining, in a receptacle, a sample containing a nucleic acid and a solid support comprising a magnetically-responsive particle, the solid support forming a complex with the nucleic acid, wherein the complex is suspended in a fluid component of the sample, the receptacle being part of a unit comprising a plurality of integrally formed receptacles, and the unit holding a removable tiplet comprising an axially extending through-hole for drawing fluids therethrough;(b) subjecting the contents of the receptacle to a magnetic field, thereby isolating the nucleic acid within the receptacle;(c) mounting the tiplet onto a fluid aspirator;(d) purifying the nucleic acid isolated in step (b) by contacting the fluid component of the sample with the tiplet and aspirating at least a portion of the fluid component with the fluid aspirator while the complex remains subjected to the magnetic field;(e) forming a reaction mixture comprising the purified nucleic acid of step (d) and all reagents required to perform a nucleic acid amplification;(f) performing a nucleic acid amplification with the reaction mixture formed in step (e), the nucleic acid amplification resulting in the enzymatic synthesis of amplification products, each of the amplification products comprising (i) a target sequence contained in the nucleic acid or (ii) the complement of the target sequence, wherein the reaction mixture is modified by the synthesis of the amplification products;(g) selectively hybridizing a detectably labeled probe to an amplification product that is one of the amplification products synthesized in step (f), thereby forming a hybrid in the modified reaction mixture that comprises the probe and the amplification product, wherein the amplification product comprises the target sequence or its complement, and wherein the probe hybridizes to the target sequence or its complement in the amplification product; and(h) while the hybrid formed in step (g) is in the modified reaction mixture, detecting the presence of the hybrid, wherein the presence of the hybrid in the modified reaction mixture is an indication of the presence of the nucleic acid in the sample,wherein the method is performed without human intervention. 18. The method of claim 17, wherein each of the receptacles comprises a cylindrical tube. 19. The method of claim 17, wherein the tiplet is electrically conductive. 20. The method of claim 19, further comprising performing in the analyzer an automated step of sensing the level of the fluid component in step (d), wherein the tiplet is mounted on an aspirator tube of the fluid aspirator, and wherein the aspirator tube and the tiplet constitute a portion of a capacitor for performing capacitive fluid level sensing. 21. The method of claim 17, wherein the tiplet is one of a plurality of tiplets held by the unit, wherein each of the tiplets is configured to permit passage of a fluid component of a sample therethrough, and wherein each of the tiplets is operationally associated with one of the receptacles of the unit. 22. The method of claim 17, wherein the tiplet is held in a frictional fit by the unit when the tiplet is mounted onto the fluid aspirator in step (c). 23. The method of claim 17, wherein the complex further comprises a capture probe, and wherein the capture probe is immobilized on the solid support and hybridized to the nucleic acid. 24. The method of claim 17 further comprising performing in the analyzer an automated step of washing the solid support one or more times with a wash buffer after step (d) and prior to step (e). 25. The method of claim 17, wherein each of the amplification products consists of a nucleic acid sequence having the target sequence or its complement. 26. The method of claim 17, wherein the amplification products include nucleic acid sequences having a sequence that is the same as the target sequence and nucleic acid sequences having a sequence that is the complement of the target sequence. 27. The method of claim 17, wherein step (f) is carried out in a temperature-controlled incubator maintained at a substantially uniform temperature. 28. The method of claim 17, further comprising performing in the analyzer, prior to step (h) and after step (g), an automated step of providing a selection reagent to the modified reaction mixture, whereby a label of an unhybridized, detectably labeled probe in the modified reaction mixture is selectively hydrolyzed, wherein the label is an acridinium ester. 29. The method of claim 17, wherein step (f) is performed in a temperature-controlled chamber of a first incubator, and wherein step (g) is performed in a temperature-controlled chamber of a second incubator, the first and second incubators being in a spaced-apart relationship. 30. The method of claim 17, wherein the hybrid is in solution in steps (g) and (h). 31. The method of claim 17, wherein the steps of the method are performed within a housing of the analyzer. 32. The method of claim 31, wherein the steps of the method are performed at multiple locations of a processing deck contained within the housing. 33. The method of claim 31, wherein the housing remains closed during the method.
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