A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancin
A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.
대표청구항▼
1. A non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding each of the following polypeptides: (i) a carbon monoxide dehydrogenase,(ii) a hydrogenase,(iii) an NAD(P)H:ferredoxin oxidoreductase, and(iv) a ferredoxin, wherein the polypeptides are expressed
1. A non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding each of the following polypeptides: (i) a carbon monoxide dehydrogenase,(ii) a hydrogenase,(iii) an NAD(P)H:ferredoxin oxidoreductase, and(iv) a ferredoxin, wherein the polypeptides are expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock; and wherein the microbial organism further comprises one or more nucleic acids encoding an enzyme selected from the group consisting of a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a pyruvate carboxylase, and a malic enzyme. 2. The non-naturally occurring microbial organism of claim 1 further comprising one or more nucleic acids encoding an enzyme selected from the group consisting of a malate dehydrogenase, a fumarase, a fumarate reductase, a succinyl-CoA synthetase, and a succinyl-CoA transferase. 3. The non-naturally occurring microbial organism of claim 1 further comprising a 1,4-butanediol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA reductase (aldehyde forming), 7) 1,4-butanediol dehydrogenase, 8) Succinate reductase, 9) Succinyl-CoA reductase (alcohol forming), 10) 4-Hydroxybutyryl-CoA transferase, 4-Hydroxybutyryl-CoA hydrolase, or 4-Hydroxybutyryl-CoA synthetase, 11) 4-Hydroxybutyrate reductase, 12) 4-Hydroxybutyryl-phosphate reductase, and 13) 4-Hydroxybutyryl-CoA reductase (alcohol forming). 4. The non-naturally occurring microbial organism of claim 1 further comprising a 1,3-butanediol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA dehydratase, 7) Crotonase, 8) 3-Hydroxybutyryl-CoA reductase (aldehyde forming), 9) 3-Hydroxybutyraldehyde reductase, 10) Succinate reductase, 11) Succinyl-CoA reductase (alcohol forming), 12) 4-Hydroxybutyryl-CoA transferase, or 4-Hydroxybutyryl-CoA synthetase, 13) 3-Hydroxybutyryl-CoA reductase (alcohol forming), 14) 3-Hydroxybutyryl-CoA hydrolase, or 3-Hydroxybutyryl-CoA synthetase, or 3-Hydroxybutyryl-CoA transferase, 15) 3-Hydroxybutyrate reductase. 5. The non-naturally occurring microbial organism of claim 1 further comprising a butanol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA dehydratase, 7) Butyryl-CoA dehydrogenase, 8) Butyryl-CoA reductase (aldehyde forming), 9) Butyraldehyde reductase, 10) Succinate reductase, 11) Succinyl-CoA reductase (alcohol forming), 12) 4-Hydroxybutyryl-CoA transferase, or 4-Hydroxybutyryl-CoA synthetase, 13) Butyryl-CoA reductase (alcohol forming), 14) Butyryl-CoA hydrolase, or Butyryl-CoA synthetase, or Butyryl-CoA transferase, 15) Butyrate reductase. 6. The non-naturally occurring microbial organism of claim 1 further comprising a 6-aminocaproic acid pathway. 7. The non-naturally occurring microbial organism of claim 1 further comprising a hexamethylenediamine pathway. 8. The non-naturally occurring microbial organism of claim 1 further comprising an adipic acid pathway. 9. The non-naturally occurring microbial organism of claim 1 further comprising a 1,3-propanediol pathway. 10. The non-naturally occurring microbial organism of claim 1 further comprising a glycerol pathway. 11. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen thereby increasing the yield of a redox-limited product via carbohydrate-based carbon feedstock, the method comprising culturing the non-naturally occurring microbial organism of claim 1 under conditions and for a sufficient period of time to produce the product. 12. A non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding each of the following polypeptides: (i) a carbon monoxide dehydrogenase,(ii) a hydrogenase,(iii) an NAD(P)H:ferredoxin oxidoreductase, and(iv) a ferredoxin, wherein the polypeptides are expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock; and wherein the microbial organism further comprises one or more nucleic acids encoding an enzyme selected from the group consisting of a fumarase or a fumarate reductase. 13. The non-naturally occurring microbial organism of claim 12 further comprising one or more nucleic acids encoding an enzyme selected from the group consisting of a malate dehydrogenase, a succinyl-CoA synthetase, and a succinyl-CoA transferase. 14. The non-naturally occurring microbial organism of claim 12 further comprising a 1,4-butanediol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA reductase (aldehyde forming), 7) 1,4-butanediol dehydrogenase, 8) Succinate reductase, 9) Succinyl-CoA reductase (alcohol forming), 10) 4-Hydroxybutyryl-CoA transferase, 4-Hydroxybutyryl-CoA hydrolase, or 4-Hydroxybutyryl-CoA synthetase, 11) 4-Hydroxybutyrate reductase, 12) 4-Hydroxybutyryl-phosphate reductase, and 13) 4-Hydroxybutyryl-CoA reductase (alcohol forming). 15. The non-naturally occurring microbial organism of claim 12 further comprising a 1,3-butanediol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA dehydratase, 7) Crotonase, 8) 3-Hydroxybutyryl-CoA reductase (aldehyde forming), 9) 3-Hydroxybutyraldehyde reductase, 10) Succinate reductase, 11) Succinyl-CoA reductase (alcohol forming), 12) 4-Hydroxybutyryl-CoA transferase, or 4-Hydroxybutyryl-CoA synthetase, 13) 3-Hydroxybutyryl-CoA reductase (alcohol forming), 14) 3-Hydroxybutyryl-CoA hydrolase, or 3-Hydroxybutyryl-CoA synthetase, or 3-Hydroxybutyryl-CoA transferase, 15) 3-Hydroxybutyrate reductase. 16. The non-naturally occurring microbial organism of claim 12 further comprising a butanol pathway, and wherein said microbial organism further comprises at least one exogenous nucleic acid encoding an enzyme selected from the group consisting of: 1) Succinyl-CoA transferase, or Succinyl-CoA synthetase (or succinyl-CoA ligase), 2) Succinyl-CoA reductase (aldehyde forming), 3) 4-Hydroxybutyrate dehydrogenase, 4) 4-Hydroxybutyrate kinase, 5) Phosphotrans-4-hydroxybutyrylase, 6) 4-Hydroxybutyryl-CoA dehydratase, 7) Butyryl-CoA dehydrogenase, 8) Butyryl-CoA reductase (aldehyde forming), 9) Butyraldehyde reductase, 10) Succinate reductase, 11) Succinyl-CoA reductase (alcohol forming), 12) 4-Hydroxybutyryl-CoA transferase, or 4-Hydroxybutyryl-CoA synthetase, 13) Butyryl-CoA reductase (alcohol forming), 14) Butyryl-CoA hydrolase, or Butyryl-CoA synthetase, or Butyryl-CoA transferase, 15) Butyrate reductase. 17. The non-naturally occurring microbial organism of claim 12 further comprising a 6-aminocaproic acid pathway. 18. The non-naturally occurring microbial organism of claim 12 further comprising a hexamethylenediamine pathway. 19. The non-naturally occurring microbial organism of claim 12 further comprising an adipic acid pathway. 20. The non-naturally occurring microbial organism of claim 12 further comprising a 1,3-propanediol pathway 21. The non-naturally occurring microbial organism of claim 12 further comprising a glycerol pathway. 22. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen thereby increasing the yield of a redox-limited product via carbohydrate-based carbon feedstock, the method comprising culturing the non-naturally occurring microbial organism of claim 12 under conditions and for a sufficient period of time to produce the product.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (156)
Gokarn,Ravi R.; Selifonova,Olga V.; Jessen,Holly Jean; Gort,Steven John; Selmer,Thorsten; Buckel,Wolfgang, 3-Hydroxypropionic acid and other organic compounds.
Gaddy James L. (Fayetteville AR) Clausen Edgar C. (Fayetteville AR), Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism.
Severson David K. (Manitowoc WI) Barrett Cheryl L. (Manitowoc WI), Lactobacillus delbrueckii ssp. bulgaricus strain and fermentation process for producing L-(+)-lactic acid.
Frost John W. (West Lafayette IN) Draths Karen M. (West Lafayette IN), Bacterial cell tranformants for production of cis, cis-muconic acid and catechol.
Raemakers Franken,Petronella C.; Nossin,Petrus M. M.; Brandts,Paul M.; Wubbolts,Marcel G.; Peeters,Wijnand P. H.; Ernste,Sandra; Wildeman De,Stefaan M. A.; Schuermann,Martin, Biochemical synthesis of 6-amino caproic acid.
Laffend Lisa Anne (Wilmington DE) Nagarajan Vasantha (Wilmington DE) Nakamura Charles Edwin (Claymont DE), Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism.
Huisman Gjalt W. ; Skraly Frank ; Martin David P. ; Peoples Oliver P., Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids.
Huisman, Gjalt W.; Skraly, Frank; Martin, David P.; Peoples, Oliver P., Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids.
Tobin Allan J. (Los Angeles CA) Erlander Mark G. (Tarzana CA) Kaufman Daniel L. (Santa Monica CA) Clare-Salzler Michael J. (Los Angeles CA), Cloned glutamic acid decarboxylase peptides.
Burdette Douglas S. ; Zeikus Joseph G., Cloning and expression of the gene encoding thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and enzy.
Berry, David A.; Robertson, Dan E.; Skraly, Frank A.; Green, Brian D.; Ridley, Christian P.; Kosuri, Sriram; Reppas, Nikos B.; Sholl, Martha; Afeyan, Noubar B., Engineered CO2 fixing microorganisms producing carbon-based products of interest.
Davis,S. Christopher; Grate,John H.; Gray,David R.; Gruber,John M.; Huisman,Gjalt W.; Ma,Steven K.; Newman,Lisa M.; Sheldon,Roger; Wang,Li A, Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives.
Davis,S. Christopher; Grate,John H.; Gray,David R.; Gruber,John M.; Huisman,Gjalt W.; Ma,Steven K.; Newman,Lisa M.; Sheldon,Roger; Wang,Li A, Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters.
Cao,Yongwei; Hinkle,Gregory J.; Slater,Steven C.; Chen,Xianfeng; Goldman,Barry S., Expression of microbial proteins in plants for production of plants with improved properties.
Datta Rathin (442 W. Melrose Ave. ; #3 Chicago IL 60657) Glassner David A. (4454 Satinwood Rd. Okemos MI 48864) Jain Mahendra K. (3950 Jonquil La. Okemos MI 48864) Vick Roy John R. (1642 Seven Trails, Fermentation and purification process for succinic acid.
Rieping, Mechthild; Bastuck, Christine; Hermann, Thomas; Thierbach, Georg, Fermentation process for the preparation of L-amino acids using strains of the family Enterobacteriaceae.
Lee,Sang Yup; Song,Hyohak; Jang,Yu Sin; Lim,Sung Won, Gene encoding formate dehydrogenases D & E and method for preparing succinic acid using the same.
Tobin Allan J. ; Erlander Mark G. ; Kaufman Daniel L., Hybridomas and monoclonal antibodies that specifically bind to glutamic acid decarboxylase peptides.
Broecker Franz Josef (Ludwigshafen DT) Schwarzmann Matthias (Limburgerhof DT), Manufacture of butanediol and/or tetrahydrofuran from maleic and/or succinic anhydride via g
상세보기
Ravi R. Gokarn ; Mark A. Eiteman ; Elliot Altman, Metabolically engineered E. coli for enhanced production of oxaloacetate-derived biochemicals.
Donnelly Mark I. ; Sanville-Millard Cynthia ; Chatterjee Ranjini, Method for construction of bacterial strains with increased succinic acid production.
Guettler Michael V. (Holt MI) Jain Mahendra K. (Okemos MI), Method for making succinic acid, anaerobiospirillum succiniciproducens variants for use in process and methods for obtai.
Guettler Michael V. (Holt MI) Jain Mahendra K. (Okemos MI) Rumler Denise (Leslie MI), Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants.
Ben A. Bulthuis NL; Gregory M. Whited ; Donald E. Trimbur ; Anthony A. Gatenby, Method for the production of 1,3-propanediol by recombinant organisms comprising genes for vitamin B12 transport.
Allan J. Tobin ; Mark G. Erlander ; Daniel L. Kaufman, Methods and kits useful for determining the status of and detecting pancreatic B-cell associated autoimmune diseases.
Gaddy,James L.; Arora,Dinesh K.; Ko,Ching Whan; Phillips,John Randall; Basu,Rahul; Wikstrom,Carl V.; Clausen,Edgar C., Methods for increasing the production of ethanol from microbial fermentation.
Burk, Mark J.; Pharkya, Priti; Van Dien, Stephen J.; Burgard, Anthony P.; Schilling, Christophe H., Methods for the synthesis of olefins and derivatives.
Gruys Kenneth James ; Mitsky Timothy Albert ; Kishore Ganesh Murthy ; Slater Steven Charles ; Padgette Stephen Rogers ; Stark David Martin, Methods of optimizing substrate pools and biosynthesis of poly-.beta.-hydroxybutyrate-co-poly-.beta.-hydroxyvalerate in.
Gruys Kenneth James ; Mitsky Timothy Albert ; Kishore Ganesh Murthy ; Slater Steven Charles ; Padgette Stephen Rogers ; Stark David Martin, Methods of optimizing substrate pools and biosynthesis of poly-.beta.-hydroxybutyrate-co-poly-.beta.-hydroxyvalerate in.
Timothy A. Mitsky ; Steven C. Slater ; Steven E. Reiser ; Ming Hao ; Kathryn L. Houmiel, Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways.
Jain Mahendra K. (Okemos MI) Beacom Daniel (East Lansing MI) Datta Rathin (Chicago IL), Mutant strain of C. acetobutylicum and process for making butanol.
Steinbuchel Alexander,DEX ; Liebergesell Mathias,DEX ; Valentin Henry ; Pries Andreas,DEX, PHA E and PHA C components of poly(hydroxy fatty acid) synthase from thiocapsa pfennigii.
Tobin Allan J. (Los Angeles CA) Erlander Mark G. (Tarzana CA) Kaufman Daniel L. (Santa Monica CA) Clare-Salzler Michael J. (Gainesville FL), Peptides derived from glutamic acid decarboxylase.
Cannon, Maura; Cannon, Francis C.; McCool, Gabriel J.; Valentin, Henry E.; Gruys, Kenneth J., Polyhydroxyalkanoate biosynthesis associated proteins and coding region in bacillus megaterium.
Ward ; Jr. N. Robert (Seattle WA) DesRosier John P. (Seattle WA) Marshall ; III Elliott D. (Renton WA) Ford Judith (Bothell WA) Mallinak Nancy J. S. (Seattle WA), Precipitate test for microorganisms.
Guettler Michael V. (Holt MI) Jain Mahendra K. (Okemos MI) Soni Bhupendra K. (Westmont IL), Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms.
Matsuyama Akinobu (Niigata JPX) Nikaido Teruyuki (Niigata JPX) Kobayashi Yoshinori (Niigata JPX), Process for producing optically active 1,3-butanediol by reduction of 4-hydroxy-2-butanone.
Emptage,Mark; Haynie,Sharon L.; Laffend,Lisa A.; Pucci,Jeff P.; Whited,Gregory, Process for the biological production of 1,3-propanediol with high titer.
Emptage,Mark; Haynie,Sharon L.; Laffend,Lisa A.; Pucci,Jeff P.; Whited,Gregory Marshall, Process for the biological production of 1,3-propanediol with high titer.
Gottschalk G. (Nrtenhardenberg DEX) Averhoff Beate (Gottingen DEX), Process for the microbiological preparation of 1,3-propane-diol from glycerol by citrobacter.
Inoue Shigeo (Utsunomiya JPX) Kimura Yoshiharu (Utsunomiya JPX) Adachi Shigehito (Ichikai JPX), Process for the preparation of dicarboxylic acid using microorganism.
Wolters Henricus F. W. (Echt NLX) Lane Samuel L. (Beaumont TX) Buijs Wim (Schinnen NLX) Haasen Nicolaas F. (Sittard NLX) Herkes Frank E. (Wilmington DE), Process for the preparation of epsilon-caprolactam and epsilon-caprolactam precursors.
Glassner David A. (4454 Satinwood Rd. Okemos MI 48864) Datta Rathin (442 W. Melrose Ave. ; #3 Chicago IL 60657), Process for the production and purification of succinic acid.
Rieping,Mechthild; Hermann,Thomas, Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene.
Somerville Christopher R. (Portola Valley CA) Nawrath Christiane (Palo Alto CA) Poirier Yves (Palo Alto CA), Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants.
Vineet Rajgarhia ; Vassily Hatzimanikatis ; Stacey Olson ; Ting Carlson ; John N. Starr ; Jeffrey J. Kolstad ; Aharon Eyal IL, Production of lactate using crabtree negative organisms in varying culture conditions.
Jao Go Pan KR; Soo An Shin KR; Chan Kyu Park KR; Pil Kim KR; Dong Eun Chang KR; Jae Eun Kim KR, Pta LDHA double mutant Escherichia coli SS373 and the method of producing succinic acid therefrom.
Hespell Robert B. ; Wyckoff Herbert A. ; Dien Bruce S. ; Bothast Rodney J., Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities.
Somerville Christopher R. (Okemos MI) Poirier Yves (East Lansing MI) Dennis Douglas E. (Weyers Cave VA), Transgenic plants producing polyhydroxyalkanoates.
Burgard, Anthony P.; Osterhout, Robin E.; Van Dien, Stephen J.; Tracewell, Cara Ann; Pharkya, Priti; Andrae, Stefan, Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing adipate, 6-aminocaproate, hexamethylenediamine or caprolactam related thereto.
Burgard, Anthony P.; Burk, Mark J.; Osterhout, Robin E.; Sun, Jun; Pharkya, Priti, Microorganisms for producing 1,3-butanediol and methods related thereto.
Tracy, Bryan P.; Jones, Shawn William; Eyal, Aharon M., Mixotrophic fermentation method for making acetone, isopropanol, butyric acid and other bioproducts, and mixtures thereof.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.