IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0773825
(2010-05-04)
|
등록번호 |
US-8445546
(2013-05-21)
|
발명자
/ 주소 |
- Wood, Anthony B.
- Watson, Richard L.
- Archambeau, Gregory J.
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
13 인용 특허 :
322 |
초록
▼
Provided are electrokinetically-altered fluids (e.g., gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellul
Provided are electrokinetically-altered fluids (e.g., gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellular membrane potential and cellular membrane conductivity. Further provided are the methods of making the electrokinetically-altered ionic aqueous fluid compositions. Particular aspects provide for regulating or modulating intracellular signal transduction associated by modulation of at least one of cellular membranes, membrane potential, membrane proteins such as membrane receptors, including but not limited to G-Protein Coupled Receptors (GPCR), and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes). Other embodiments include particular methods of producing the electrokinetically-altered fluids. The electrokinetically-altered fluid compositions and methods of producing the fluid include electrokinetically-altered ionic aqueous fluids optionally in the form of solvated electrons stabilized, at least in part, with molecular gas (e.g., oxygen).
대표청구항
▼
1. A electrokinetically-altered fluid composition, comprising an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in
1. A electrokinetically-altered fluid composition, comprising an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity. 2. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized gas-containing nanostructures comprise charge-stabilized oxygen gas-containing nanostructure. 3. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized gas-containing nanostructures comprise charge-stabilized nitrogen gas-containing nanostructures. 4. The electrokinetically-altered fluid composition of claim 2, wherein the percentage of dissolved oxygen molecules present in the fluid as the charge-stabilized oxygen gas-containing nanostructures is a percentage. 5. The electrokinetically-altered fluid composition of claim 1, wherein the percentage of dissolved oxygen molecules present in the fluid as the charge-stabilized oxygen gas-containing nanostructures is a percentage greater than 50%. 6. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized gas-containing nanostructures have an average diameter of less than 90 nm. 7. The electrokinetically-altered fluid composition of claim 1, wherein the ionic aqueous solution comprises a saline solution. 8. The electrokinetically-altered fluid composition of claim 1, wherein the fluid is super-gasified relative to the gas concentration of the fluid at atmospheric pressure and ambient temperature. 9. The electrokinetically-altered fluid composition of claim 1, wherein the charge-stabilized gas-containing nanostructures comprise at least one positively-charged ion selected from the group consisting of alkali metal based salts including Li+, Na+, K+, Rb+, and Cs+, alkaline earth based salts including Mg++ and Ca++, and transition metal-based positive ions including Cr, Fe, Co, Ni, Cu, and Zn, in each case along with any suitable counterionic components. 10. The electrokinetically-altered fluid composition of claim 1, wherein the fluid comprises at least one of a form of solvated electrons, and an electrokinetically modified or charged oxygen species. 11. The electrokinetically-altered fluid composition of claim 10, wherein the form of solvated electrons or electrokinetically modified or charged oxygen species are present in an amount of at least 0.01 ppm. 12. The electrokinetically-altered fluid composition of claim 10, wherein the electrokinetically-altered fluid comprises a form of solvated electrons stabilized, at least in part, by molecular oxygen. 13. The electrokinetically-altered fluid composition of claim 1, wherein the ability to modulation of at least one of cellular membrane potential and cellular membrane conductivity persists for at least two months in a closed gas-tight container. 14. The electrokinetically-altered fluid composition of claim 1, wherein alteration of the electrokinetically altered aqueous fluid comprises exposure of the fluid to hydrodynamically-induced, localized electrokinetic effects. 15. The electrokinetically-altered fluid composition of claim 14, wherein, exposure to the localized electrokinetic effects comprises exposure to at least one of voltage pulses and current pulses. 16. The electrokinetically-altered fluid composition of claim 14, wherein the exposure of the fluid to hydrodynamically-induced, localized electrokinetic effects, comprises exposure of the fluid to electrokinetic effect-inducing structural features of a device used to generate the fluid. 17. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises altering of a conformation, ligand binding activity, or a catalytic activity of a membrane associated protein. 18. The electrokinetically-altered fluid composition of claim 17, wherein the membrane associated protein comprises at least one selected from the group consisting of receptors, transmembrane receptors, ion channel proteins, intracellular attachment proteins, cellular adhesion proteins and integrins. 19. The electrokinetically-altered fluid composition of claim 18, wherein the transmembrane receptor comprises a G-Protein Coupled Receptor (GPCR). 20. The electrokinetically-altered fluid composition of claim 19, wherein the G-Protein Coupled Receptor (GPCR) interacts with a G protein α subunit. 21. The electrokinetically-altered fluid composition of claim 20, wherein the G protein α subunit comprises at least one selected from the group consisting of Gαs, Gαi, Gαq, and Gα12. 22. The electrokinetically-altered fluid composition of claim 21, wherein the at least one G protein α subunit is Gαq. 23. The electrokinetically-altered fluid composition of claim 20, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity, comprises modulating whole-cell conductance. 24. The electrokinetically-altered fluid composition of claim 23, wherein modulating whole-cell conductance, comprises modulating at least one of a linear or non-linear voltage-dependent contribution of the whole-cell conductance. 25. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of a calcium dependent cellular messaging pathway or system. 26. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of phospholipase C activity. 27. The electrokinetically-altered fluid composition of claim 1, wherein modulation of at least one of cellular membrane potential and cellular membrane conductivity comprises modulation of adenylate cyclase (AC) activity. 28. The electrokinetically-altered fluid composition of claim 1, wherein the electrokinetically-altered aqueous fluid comprises dissolved gas in an amount of at least 8 ppm at atmospheric pressure. 29. The electrokinetically-altered fluid composition of claim 28, wherein the gas in the charge-stabilized nanostructures of the fluid or solution is present in an amount of at least 25 ppm. 30. A method of producing an electrokinetically-altered fluid, comprising: providing a flow of a fluid material between two spaced surfaces in relative motion and defining a mixing volume therebetween, wherein the dwell time of a single pass of the flowing fluid material within and through the mixing volume is greater than 0.06 seconds or greater than 0.1 seconds; andintroducing gas into the flowing fluid material within the mixing volume under conditions suitable to dissolve at least 20 ppm into the material, and electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 31. The method of claim 30, wherein the gas is infused into the material in less than 100 milliseconds. 32. A method of producing an electrokinetically-altered fluid, comprising: providing a flow of a fluid material between two spaced surfaces defining a mixing volume therebetween; andintroducing gas into the flowing material within the mixing volume under conditions suitable to infuse at least 20 ppm gas into the material in less than 100 milliseconds, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 33. The method of claim 32, wherein the dwell time of the flowing material within the mixing volume is greater than 0.06 seconds. 34. The method of any one of claims 30, 31, 32 and 33, wherein the ratio of surface area to the volume is at least 12. 35. A method of producing an electrokinetically-altered fluid, comprising use of a mixing device for creating an output mixture by mixing a first material and a second material, the device comprising: a first chamber configured to receive the first material from a source of the first material;a stator;a rotor having an axis of rotation, the rotor being disposed inside the stator and configured to rotate about the axis of rotation therein, at least one of the rotor and stator having a plurality of through-holes;a mixing chamber defined between the rotor and the stator, the mixing chamber being in fluid communication with the first chamber and configured to receive the first material therefrom, and the second material being provided to the mixing chamber via the plurality of through-holes formed in the one of the rotor and stator;a second chamber in fluid communication with the mixing chamber and configured to receive the output material therefrom; anda first internal pump housed inside the first chamber, the first internal pump being configured to pump the first material from the first chamber into the mixing chamber, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 36. A method of producing an electrokinetically-altered fluid, comprising use of a mixing device for creating an output mixture by mixing a first material and a second material, the device comprising: a stator;a rotor having an axis of rotation, the rotor being disposed inside the stator and configured to rotate about the axis of rotation therein;a mixing chamber defined between the rotor and the stator, the mixing chamber having an open first end through which the first material enters the mixing chamber and an open second end through which the output material exits the mixing chamber, the second material entering the mixing chamber through at least one of the rotor and the stator;a first chamber in communication with at least a majority portion of the open first end of the mixing chamber; anda second chamber in communication with the open second end of the mixing chamber, to electrokinetically alter the fluid or solution, wherein an electrokinetically altered aqueous fluid comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures having an average diameter of less than 100 nanometers and stably configured in the ionic aqueous fluid in an amount sufficient to provide, upon contact of a living cell by the fluid, modulation of at least one of cellular membrane potential and cellular membrane conductivity is provided. 37. The method of claim 35, wherein the first internal pump is configured to impart a circumferential velocity into the first material before it enters the mixing chamber. 38. The composition of claim 1, wherein the charge-stabilized gas-containing nanostructures of the electrokinetically-altered fluid comprise at least one positively-charged ion selected from the group consisting of alkali metal based salts including Li+, Na+, K+, Rb+, and Cs+, alkaline earth based salts including Mg++ and Ca++, and transition metal-based positive ions including Cr, Fe, Co, Ni, Cu, and Zn. 39. The composition of claim 1, wherein the charge-stabilized gas-containing nanostructures comprise at least one of nanocages, nanoclusters and nanobubbles. 40. The composition of claim 38, comprising saline or oxygen gas enriched saline.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.