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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0806945 (2010-08-24) |
등록번호 | US-8497063 (2013-07-30) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 1 인용 특허 : 473 |
A sex selected equine embryo production system for the production of sex selected equine embryos and offspring by fertilization of oocytes with sex selected sperm by intracytoplasmic sperm injection.
1. A method of producing an equine embryo of a selected sex comprising: (a) obtaining a plurality of equine sperm cells;(b) determining a sex of said plurality of equine sperm cells;(c) sorting said plurality of equine sperm cells based upon sex;(d) obtaining an equine oocyte;(e) intracytoplasmicall
1. A method of producing an equine embryo of a selected sex comprising: (a) obtaining a plurality of equine sperm cells;(b) determining a sex of said plurality of equine sperm cells;(c) sorting said plurality of equine sperm cells based upon sex;(d) obtaining an equine oocyte;(e) intracytoplasmically injecting said equine oocyte with one of said plurality of equine sperm cells of a selected sex; and(f) producing an equine embryo of said selected sex, wherein said equine embryo is capable of blastocyst formation. 2. The method of claim 1, further comprising freezing said plurality of equine sperm cells sorted based upon sex. 3. The method of claim 2, further comprising thawing said plurality of equine sperm cells. 4. The method of claim 3, further comprising washing said plurality of equine sperm cells. 5. The method of claim 4, wherein said washing comprises washing said plurality of equine sperm in a chemically defined medium containing heparin. 6. The method of claim 4, further comprising swimming up said equine sperm cells prior to said washing. 7. The method of claim 6, wherein said swimming up comprises swimming up of said equine sperm cells in condition defined medium containing about 2 mM caffeine and heparin. 8. The method of claim 1, further comprising utilizing a piezo injection system for said injecting said one of said plurality of equine sperm cells of a selected sex into said equine oocyte. 9. The method of claim 8, further comprising providing a sperm-injection pipette having an external diameter of about eight to ten micrometers. 10. The method of claim 1, further comprising providing a flow cytometer which performs said determining a sex of said plurality of equine sperm cells and said sorting said plurality of equine sperm cells based upon sex. 11. The method of claim 10, further comprising staining an amount of DNA contained within said plurality of equine sperm cells with a DNA selective material prior to said determining a sex of said plurality of equine sperm cells. 12. The method of claim 11, further comprising exposing said plurality of equine sperm cells to a light source to generate an amount of fluorescence which varies based on the amount of said DNA staining with said DNA selective material. 13. The method of claim 12, further comprising detecting said amount of fluorescence which varies based on the amount of said DNA stained with said DNA selective material.
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