Method of providing portable biological testing capabilities
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/00
C12M-001/34
출원번호
US-0032396
(2011-02-22)
등록번호
US-8518637
(2013-08-27)
발명자
/ 주소
Barnes, Allen C.
Barnes, Janice
출원인 / 주소
Barnes, Allen C.
대리인 / 주소
Knobbe, Martens, Olson & Bear LLP
인용정보
피인용 횟수 :
0인용 특허 :
53
초록▼
A method for providing portable biological testing capabilities free from biological contamination from an environment outside the device is provided. The method includes providing components configured to be assembled together to seal a volume against passage of biological materials between the vol
A method for providing portable biological testing capabilities free from biological contamination from an environment outside the device is provided. The method includes providing components configured to be assembled together to seal a volume against passage of biological materials between the volume and an environment outside the volume. The method further includes sterilizing the components and providing a sterilized culture medium. The method further includes assembling the components together with the sterilized culture medium within the volume. The method further includes sterilizing the assembled components by elevating the temperature. The method further includes flowing gas from within the volume to the environment while at an elevated temperature. The method further includes reducing the temperature to be less than the elevated temperature while preventing gas from flowing from the environment to the volume, thereby creating a pressure within the volume which is less than a pressure outside the volume.
대표청구항▼
1. A method of providing portable biological testing capabilities free from biological contamination from a local environment, the method comprising: providing components of a portable device, the components configured to be assembled together to seal a volume within the device against passage of bi
1. A method of providing portable biological testing capabilities free from biological contamination from a local environment, the method comprising: providing components of a portable device, the components configured to be assembled together to seal a volume within the device against passage of biological materials between the volume and an environment outside the device;sterilizing the components;providing a sterilized culture medium;assembling the components together with the sterilized culture medium within the volume, thereby forming an assembled device;sterilizing the assembled device, wherein sterilizing the assembled device comprises elevating a temperature of the assembled device;flowing gas from within the volume to the environment while the assembled device is at an elevated temperature due to said sterilizing the assembled device; andreducing the temperature of the assembled device to be less than the elevated temperature while preventing gas from flowing from the environment to the volume, thereby creating a pressure within the volume which is less than a pressure outside the volume. 2. The method of claim 1, wherein sterilizing the components comprises exposing the components to gamma radiation or ultraviolet radiation. 3. The method of claim 1, wherein sterilizing the assembled device comprises exposing the assembled device to gamma radiation or ultraviolet radiation. 4. The method of claim 1, further comprising: providing a desiccant material;placing the assembled device and the desiccant material within a container; andsealing the container against passage of biological materials and water vapor between the assembled device and a region outside the container, wherein sterilizing the assembled device is performed while the assembled device is sealed within the container. 5. The method of claim 1, wherein the assembled device comprises a valve in fluidic communication with the volume and the environment, wherein flowing gas from within the volume to the environment comprises opening the valve, and preventing gas from flowing from the environment to the volume comprises closing the valve. 6. The method of claim 5, wherein the valve opens in response to an elevated pressure within the volume and closes once there is no longer a pressure differential force keeping the valve open. 7. The method of claim 5, wherein the valve comprises a hole through a housing of the assembled device and a flexible layer covering the hole, wherein a portion of the flexible layer is configured to flex away from the hole in response to pressure within the volume being greater than pressure within the environment. 8. The method of claim 1, wherein the assembled device comprises a growth medium while the assembled device is being sterilized. 9. A method of providing a sterilized volume with a reduced pressure, the method comprising: providing a device comprising: a volume sealed against passage of biological material between the volume and a region outside the volume; anda valve which can be closed or opened, the valve inhibiting gas from flowing from the region to the volume when closed, the valve allowing gas to flow from the volume to the region when opened, wherein the valve opens in response to a pressure within the volume being greater than a pressure within the region;sterilizing the volume, wherein said sterilizing increases a temperature within the volume and increases the pressure within the volume to be greater than the pressure within the region;opening the valve in response to the increased pressure within the volume, thereby allowing gas to flow through the valve from the volume to the region; andcooling the volume and closing the valve, wherein said cooling decreases the pressure within the volume to create a pressure differential across the valve. 10. The method of claim 9, wherein sterilizing the volume comprises irradiating the volume with gamma radiation or ultraviolet radiation. 11. The method of claim 9, further comprising: providing a desiccant material;placing the device and the desiccant material within a container; andsealing the container against passage of biological materials and water vapor between the device and a region outside the container, wherein sterilizing the volume is performed while the device is sealed within the container. 12. The method of claim 9, wherein the valve comprises a hole through a housing of the device and a flexible layer covering the hole, wherein a portion of the flexible layer is configured to flex away from the hole in response to pressure within the volume being greater than pressure within the environment. 13. The method of claim 9, wherein the valve opens in response to an elevated pressure within the volume and closes once there is no longer a pressure differential force keeping the valve open. 14. The method of claim 9, wherein the device comprises a growth medium while the volume is being sterilized. 15. A method of using a biological testing device, the method comprising: providing a device comprising: a housing;a volume surrounded by the housing and sealed against passage of biological materials between the volume and the environment outside the device;a growth medium within the volume;a port configured to provide access to the volume while avoiding biological contamination of the volume; andone or more channels within the volume, the one or more channels in fluidic communication with the port, with the culture medium, and with a region of the volume above the culture medium;a valve in fluidic communication with the volume and the environment, the valve having an open state in which gas flows from within the volume to the environment outside the device and having a closed state in which gas is inhibited from flowing between the volume and the environment, wherein the valve is in the open state in response to a pressure within the volume larger than a pressure of the environment outside the device, thereby reducing the pressure within the volume;elevating a temperature of the volume;opening the valve while the volume is at an elevated temperature;reducing the temperature of the volume while the valve is closed, thereby reducing a pressure within the volume;introducing a liquid specimen to the port at an inlet pressure; andflowing the liquid specimen from the port, through the one or more channels, to the culture medium, wherein the flowing of the liquid specimen is facilitated by a pressure differential force between the inlet pressure at the port and the reduced pressure within the volume. 16. The method of claim 15, wherein elevating the temperature of the volume comprises sterilizing the volume. 17. The method of claim 16, wherein sterilizing the volume comprises exposing the volume to gamma radiation or ultraviolet radiation. 18. The method of claim 16, further comprising: providing a desiccant material;placing the volume and the desiccant material within a container; andsealing the container against passage of biological materials and water vapor between the volume and a region outside the container, wherein sterilizing the volume is performed while the volume is sealed within the container. 19. The method of claim 15, wherein the valve closes once there is no longer a pressure differential force keeping the valve open. 20. The method of claim 15, wherein the valve comprises a hole through the housing and a flexible layer covering the hole, wherein a portion of the flexible layer is configured to flex away from the hole in response to pressure within the volume being greater than pressure within the environment.
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