Hydrolases, nucleic acids encoding them and methods for biocatalytic synthesis of structured lipids
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/34
C12Q-001/00
C21N-009/14
C21N-001/20
C21N-015/00
C12P-007/00
C12P-021/04
C12P-021/06
C07H-021/04
출원번호
US-0083480
(2011-04-08)
등록번호
US-8541191
(2013-09-24)
발명자
/ 주소
Dayton, Christopher L. G.
Hitchman, Tim
Kline, Katie
Lyon, Jonathan
Wall, Mark A.
Barton, Nelson R.
출원인 / 주소
Bunge Oils, Inc.
대리인 / 주소
Jones Day
인용정보
피인용 횟수 :
0인용 특허 :
132
초록▼
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, pa
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
대표청구항▼
1. A method of producing a diacylglyceride, the method comprising the steps of: (a) providing an oil composition comprising a quantity of triacylglyceride,(b) providing a polypeptide having a palmitase activity, said polypeptide being selected from the group consisting of isolated, synthetic and rec
1. A method of producing a diacylglyceride, the method comprising the steps of: (a) providing an oil composition comprising a quantity of triacylglyceride,(b) providing a polypeptide having a palmitase activity, said polypeptide being selected from the group consisting of isolated, synthetic and recombinant polypeptides having a palmitase activity and said polypeptide either i. being encoded by a nucleic acid comprising a nucleic acid sequence having at least 85% sequence identity to full length SEQ ID NO:1, and wherein the nucleic acid encodes at least one polypeptide having palmitase activity and the polypeptide having at least one amino acid residue change recited below in Table A, orii. having at least 85% sequence identity to full length SEQ ID NO:2, and having at least one amino acid residue change recited in Table A,Table A AminoOriginalNewAcidAminoAminoResidueAcidAcid27GQ45GL54SH61DAE62VAG72REKST74FI83VM96AI98GL116EAFHLQQR, H140RRSSTV117LR, I161L120KL133SA147IF151IAGH160PT172RQ212SR213KT214TVNRY215GAH216AVCLoriii. having at least 85% sequence identity to full length of SEQ ID NO:2 and comprising at least one amino acid residue modification D61A; D61E; R72E; R72K; E116A; E116Q; E116R; E116T; E116V; S133A; I151G; I151A; V163R; or D164R(c) contacting said oil composition of step (a) with said polypeptide of step (b) under conditions sufficient for the polypeptide to hydrolyze a palmitase residue on the triacylglyceride to form a diacylglyceride. 2. The method of claim 1, wherein said polypeptide is Sn2 specific and said diacylglyceride is a 1,3-diacylglyceride. 3. The method of claim 1, wherein said polypeptide is Sn1 or Sn3 specific and said diacylglyceride is a 1,2-diacylglyceride or a 2,3-diacylglyceride. 4. The method of claim 1, wherein the polypeptide has at least 85% sequence identity to full length SEQ ID NO:2, and has at least one amino acid residue change recited in Table A. 5. The method of claim 1, wherein the polypeptide has at least 85% sequence identity to full length SEQ ID NO:2 and further comprises at least one of amino acid residue modification D61A; D61E; R72E; R72K; E116A; E116Q; E116R; E116T; E116V; S133A; I151G; 1151A; V163R; or D164R. 6. The method of claim 1, wherein the polypeptide has at least 85% sequence identity to full length SEQ ID NO:2 and further comprises at least one of amino acid residue modification I20L; V62S; G77P; V83C; D88H; Y113G; E116T; E116G; H140K; K146S; I167S; L180E; E194M; A211Q; S212Y; G215C; G215V; G215W; A218H; A218S; V223A; A225M; or A225Q.
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