Microorganism concentration process and agent
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-001/00
C12N-011/00
C12N-011/14
C12N-011/16
C12Q-001/00
C12Q-001/02
C12Q-001/24
C12Q-001/68
출원번호
US-0678579
(2008-10-02)
등록번호
US-8546100
(2013-10-01)
국제출원번호
PCT/US2008/078575
(2008-10-02)
§371/§102 date
20100317
(20100317)
국제공개번호
WO2009/046191
(2009-04-09)
발명자
/ 주소
Kshirsagar, Manjiri T.
Kshirsagar, Tushar A.
Wood, Thomas E.
출원인 / 주소
3M Innovative Properties Company
대리인 / 주소
Crooks, Stephen L.
인용정보
피인용 횟수 :
1인용 특허 :
20
초록▼
A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale
A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
대표청구항▼
1. A process comprising (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sampl
1. A process comprising (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; (c) contacting said concentration agent with said sample such that said at least one microorganism strain is bound to or captured by said concentration agent, such that the at least one microorganism strain remains viable; and (d) detecting the presence of at least one bound microorganism strain. 2. The process of claim 1, wherein said concentration agent is a particulate concentration agent. 3. The process of claim 1, wherein said surface modifier is selected from fine-nanoscale gold, fine-nanoscale platinum, fine-nanoscale gold in combination with at least one metal oxide, titanium dioxide, titanium dioxide in combination with at least one other metal oxide, and combinations thereof. 4. The process of claim 3, wherein said metal oxide is selected from ferric oxide, titanium dioxide, zinc oxide, aluminum oxide, and combinations thereof. 5. The process of claim 3, wherein said surface modifier is selected from fine-nanoscale gold, fine-nanoscale platinum, fine-nanoscale gold in combination with at least ferric oxide or titanium dioxide, titanium dioxide, titanium dioxide in combination with at least ferric oxide, and combinations thereof. 6. The process of claim 1, wherein said sample is in the form of a fluid. 7. The process of claim 1, wherein said microorganism strain is selected from strains of bacteria, fungi, yeasts, protozoans, viruses, bacterial endospores, and combinations thereof. 8. The process of claim 7, wherein said microorganism strain is selected from strains of bacteria, yeasts, viruses, bacterial endospores, and combinations thereof. 9. The process of claim 1, wherein said contacting is carried out by mixing said concentration agent and said sample. 10. The process of claim 1, wherein said detecting is carried out by a method selected from culture-based methods, microscopy and other imaging methods, genetic detection methods, immunologic detection methods, bioluminescence-based detection methods, and combinations thereof. 11. The process of claim 1, wherein said process further comprises segregating the resulting microorganism-bound concentration agent. 12. The process of claim 11, wherein said segregating is effected by a method selected from gravitational settling, centrifugation, filtration, and combinations thereof. 13. The process of claim 12, wherein said segregating is effected at least in part by gravitational settling. 14. The process of claim 11, wherein said process further comprises separating the resulting segregated concentration agent from said sample. 15. A kit comprising (a) a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; and (b) a testing container for use in carrying out the process of claim 1.
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이 특허에 인용된 특허 (20)
Castle Richard B., At least partly fused particulates and methods of making them by flame fusion.
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Chamberlain Craig S. (Woodbury MN) Vesley George F. (Hudson MN) Zimmerman Patrick G. (St. Paul MN) McAllister Jerome W. (Hudson MN), Coated glass microbubbles and article incorporating them.
Mach Patrick A. (Shorewood MN) Hesselroth Karen E. (South St. Paul MN) Adams Carl A. (Apple Valley MN) Schwab Debra L. (Stillwater MN), Culture medium for rapid count of coliform bacteria.
Coulter Wallace H. (Miami Springs FL) Hollinger John D. (Miami FL) Russell Thomas (Miami FL) Rodriguez Carlos (Miami FL) Paul Ronald (North Miami Beach FL), Method and apparatus for rapid mixing of small volumes for enhancing biological reactions.
Coulter Wallace H. (Miami FL) Zwerner Robert K. (Ft. Lauderdale FL) Schmittling Robert J. (Cooper City FL) Russell Thomas R. (Miami FL), Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity se.
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