초록 ▼

A number of β-hemolytic streptococci polynucleotides and polypeptides, particularly Streptococcus pyogenes polypeptides and polynucleotides, are described. Two or more of the polypeptides of the invention can be formulated for use as immunogenic compositions. Also disclosed are methods for immunizin

A number of β-hemolytic streptococci polynucleotides and polypeptides, particularly Streptococcus pyogenes polypeptides and polynucleotides, are described. Two or more of the polypeptides of the invention can be formulated for use as immunogenic compositions. Also disclosed are methods for immunizing against and reducing infection caused by β-hemolytic streptococci.

대표청구항 ▼

1. An immunogenic composition comprising a mixture of two polypeptides, a first polypeptide encoded by a nucleic acid set forth as SEQ ID NO:1 and a second polypeptide encoded by a nucleic acid set forth as SEQ ID NO:3. 2. The immunogenic composition of claim 1, which further comprises a physiologic

1. An immunogenic composition comprising a mixture of two polypeptides, a first polypeptide encoded by a nucleic acid set forth as SEQ ID NO:1 and a second polypeptide encoded by a nucleic acid set forth as SEQ ID NO:3. 2. The immunogenic composition of claim 1, which further comprises a physiologically-acceptable vehicle. 3. The immunogenic composition of claim 1, which further comprises an effective amount of an adjuvant. 4. The immunogenic composition of claim 1 wherein each polypeptide is capable of generating an antibody that specifically recognizes said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in a susceptible animal. 5. The immunogenic composition of claim 1, which further comprises a physiologically-acceptable vehicle. 6. The immunogenic composition of claim 1, which further comprises an effective amount of an adjuvant. 7. The immunogenic composition of claim 4, wherein the β-hemolytic streptococci is Group A streptococci. 8. The immunogenic composition of claim 7, wherein the Group A streptococci is Streptococcus pyogenes. 9. An immunogenic composition comprising a mixture of two polypeptides, a first polypeptide having the amino acid sequence set forth as SEQ ID NO:2 and a second polypeptide having the amino acid sequence set forth as SEQ ID NO:4. 10. The immunogenic composition of claim 9, which further comprises a physiologically-acceptable vehicle. 11. The immunogenic composition of claim 9, which further comprises an effective amount of an adjuvant. 12. The immunogenic composition of claim 9, wherein each polypeptide is capable of generating an antibody that specifically recognizes said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in a susceptible animal. 13. The immunogenic composition of claim 12, which further comprises a physiologically-acceptable vehicle. 14. The immunogenic composition of claim 12, which further comprises an effective amount of an adjuvant. 15. The immunogenic composition of claim 12, wherein the β-hemolytic streptococci is Group A streptococci. 16. The immunogenic composition of claim 15, wherein the Group A streptococci is Streptococcus pyogenes. 17. A method of treating a susceptible animal against colonization or infection by β-hemolytic streptococci comprising administering to the mammal an effective amount of the immunogenic composition of claim 1, wherein each polypeptide is capable of generating an antibody specific to said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in the susceptible animal. 18. The method of claim 17, wherein the immunogenic composition is administered by subcutaneous injection, by intramuscular injection, by oral ingestion, intranasally, or combinations thereof. 19. The method of claim 17 wherein the β-hemolytic streptococci is Group A streptococci. 20. The method of claim 19 wherein the Group A streptococci is Streptococcus pyogenes. 21. A method of treating a susceptible animal against colonization or infection by β-hemolytic streptococci comprising administering to the mammal an effective amount of the immunogenic composition of claim 9, wherein each polypeptide is capable of generating an antibody specific to said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in the susceptible mammal animal. 22. The method of claim 21, wherein the immunogenic composition is administered by subcutaneous injection, by intramuscular injection, by oral ingestion, intranasally, or combinations thereof. 23. The method of claim 21 wherein the streptococci is Group A streptococci. 24. The method of claim 23 wherein the Group A streptococci is Streptococcus pyogenes. 25. The immunogenic composition of clam 1 further comprising at least one other polypeptide encoded by nucleic acid set forth as SEQ. ID. NO: 5, 7 or 9. 26. The immunogenic composition of claim 25, which further comprises a physiologically-acceptable vehicle. 27. The immunogenic composition of claim 25, which further comprises an effective amount of an adjuvant. 28. The immunogenic composition of claim 25 wherein each polypeptide is capable of generating an antibody that specifically recognizes said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in a susceptible animal. 29. The immunogenic composition of claim 28, which further comprises a physiologically-acceptable vehicle. 30. The immunogenic composition of claim 28, which further comprises an effective amount of an adjuvant. 31. The immunogenic composition of claim 28, wherein the β-hemolytic streptococci is Group A streptococci. 32. The immunogenic composition of claim 31, wherein the Group A streptococci is Streptococcus pyogenes. 33. The immunogenic composition of clam 9 further comprising at least one other polypeptide set forth as SEQ. ID. NO: 6, 8 or 10. 34. The immunogenic composition of claim 33, which further comprises a physiologically-acceptable vehicle. 35. The immunogenic composition of claim 34, which further comprises an effective amount of an adjuvant. 36. The immunogenic composition of claim 33 wherein each polypeptide is capable of generating an antibody that specifically recognizes said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in a susceptible animal. 37. The immunogenic composition of claim 36, which further comprises a physiologically-acceptable vehicle. 38. The immunogenic composition of claim 36, which further comprises an effective amount of an adjuvant. 39. The immunogenic composition of claim 36, wherein the β-hemolytic streptococci is Group A streptococci. 40. The immunogenic composition of claim 39, wherein the Group A streptococci is Streptococcus pyogenes. 41. A method of treating a susceptible animal against colonization or infection by β-hemolytic streptococci comprising administering to the mammal an effective amount of the immunogenic composition of claim 25, wherein each polypeptide is capable of generating an antibody specific to said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in the susceptible animal. 42. The method of claim 41, wherein the immunogenic composition is administered by subcutaneous injection, by intramuscular injection, by oral ingestion, intranasally, or combinations thereof. 43. The method of claim 41 wherein the β-hemolytic streptococci is Group A streptococci. 44. The method of claim 43 wherein the Group A streptococci is Streptococcus pyogenes. 45. A method of treating a susceptible animal against colonization or infection by β-hemolytic streptococci comprising administering to the mammal an effective amount of the immunogenic composition of claim 33, wherein each polypeptide is capable of generating an antibody specific to said polypeptide, and wherein the amount of said immunogenic composition is effective to ameliorate colonization or infection by β-hemolytic streptococci in the susceptible animal. 46. The method of claim 45, wherein the immunogenic composition is administered by subcutaneous injection, by intramuscular injection, by oral ingestion, intranasally, or combinations thereof. 47. The method of claim 45 wherein the β-hemolytic streptococci is Group A streptococci. 48. The method of claim 47 wherein the Group A streptococci is Streptococcus pyogenes. 49. An immunogenic composition comprising a mixture of: (a) an SCP polypeptide having the amino acid sequence of SEQ ID NO:2;(b) a peptidylpropyl isomerase polypeptide having the amino acid sequence of SEQ ID NO:4; and(c) a putative adhesion polypeptide having the amino acid sequence of SEQ ID NO:8.