최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
---|---|
국제특허분류(IPC7판) |
|
출원번호 | US-0931722 (2007-10-31) |
등록번호 | US-8569019 (2013-10-29) |
발명자 / 주소 |
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 | 피인용 횟수 : 2 인용 특허 : 343 |
An automated process for isolating and amplifying a target nucleic acid in a self-contained analyzer.
1. A method for detecting the presence of a nucleic acid in a specimen, the method comprising the steps of: (A) providing a specimen tube containing specimen to a self-contained, standard-alone analyzer;(B) performing an automated process within a housing of the analyzer that comprises: (1) transfer
1. A method for detecting the presence of a nucleic acid in a specimen, the method comprising the steps of: (A) providing a specimen tube containing specimen to a self-contained, standard-alone analyzer;(B) performing an automated process within a housing of the analyzer that comprises: (1) transferring at least a portion of the specimen from the specimen tube to a receptacle, wherein the transferred portion of the specimen is dispensed into the receptacle with a robotic pipettor, and wherein the transferred portion of the specimen contains a nucleic acid;(2) immobilizing the nucleic acid on a solid support within the receptacle, the solid support comprising a magnetically-responsive particle;(3) applying a magnetic field to the receptacle and its contents;(4) during step (3), purifying the nucleic acid immobilized in step (2) by accessing and removing at least a portion of a fluid component of the specimen from the receptacle while the nucleic acid remains immobilized on the solid support within the receptacle;(5) forming a reaction mixture comprising the purified nucleic acid of step (4) and all reagents required to perform a nucleic acid amplification;(6) performing a nucleic acid amplification with the reaction mixture formed in step (5), the nucleic acid amplification resulting in the enzymatic synthesis of amplification products, each of the amplification products comprising (i) a target sequence contained in the nucleic acid or (ii) the complement of the target sequence;(7) selectively hybridizing a probe having a label to the target sequence or its complement of an amplification product that is one of the amplification products synthesized in step (6), thereby forming a hybrid in a mixture that comprises the probe and the amplification product; and(8) in the mixture, detecting [the label as an indication of] the formation of the hybrid, wherein the formation of the hybrid in the mixture is an indication of the presence of the nucleic acid in the specimen. 2. The method of claim 1 further comprising, prior to step (A), a step of loading the specimen tube onto a specimen tube rack. 3. The method of claim 1 further comprising, prior to step (A), a step of providing to the analyzer all reagents required to perform a nucleic acid amplification, wherein the specimen tube and the reagents required to perform a nucleic acid amplification are separately provided to the analyzer. 4. The method of claim 1, wherein the receptacle is a cylindrical tube. 5. The method of claim 1, wherein the receptacle is one of a plurality of integrally formed receptacles. 6. The method of claim 5, wherein the integrally formed receptacles are arranged in a linear array. 7. The method of claim 1, wherein the nucleic acid is immobilized on the solid support with a capture probe in step (2). 8. The method of claim 1, wherein the automated process further comprises performing within the housing of the analyzer a step of washing the solid support one or more times after step (4) and prior to step (5). 9. The method of claim 1, wherein each of the amplification products consists of a nucleic acid sequence having a sequence contained in the nucleic acid or its complement. 10. The method of claim 1, wherein the amplification products include nucleic acid sequences having a sequence that is the same as the target sequence and nucleic acid and sequences having a sequence that is the complement of the target sequence. 11. The method of claim 1, wherein step (6) is carried out in a temperature-controlled incubator maintained at a substantially uniform temperature. 12. The method of claim 1, wherein step (6) is performed in a temperature-controlled chamber of a first incubator, and wherein step (7) is performed in a temperature-controlled chamber of a second incubator, the first and second incubators being in a spaced-apart relationship. 13. The method of claim 12, wherein the hybrid is in solution. 14. The method of claim 1, wherein the label is a fluorescent or chemiluminescent label. 15. The method of claim 1, wherein the housing remains closed during the automated process. 16. The method of claim 1, wherein the automated process is performed at multiple locations of a processing deck contained within the housing. 17. A method for detecting the presence of a nucleic acid in a specimen, the method comprising the steps of: (A) providing a specimen tube containing a specimen to a self-contained, standard-alone unit analyzer;(B) performing an automated process within a housing of the analyzer that comprises: (1) transferring at least a portion of the specimen from the specimen tube to a receptacle, wherein the transferred portion of the specimen is dispensed into the receptacle with a robotic pipettor, and wherein the transferred portion of the specimen contains a nucleic acid;(2) immobilizing the nucleic acid on a solid support within the receptacle, the solid support comprising a magnetically-responsive particle;(3) applying a magnetic field to the receptacle and its contents;(4) during step (3), purifying the nucleic acid immobilized in step (2) by contacting a fluid component of the specimen with a fluid aspirator and aspirating at least a portion of the fluid component from the receptacle while the nucleic acid remains immobilized on the solid support within the receptacle;(5) forming a reaction mixture comprising the purified nucleic acid of step (4) and all reagents required to perform a nucleic acid amplification;(6) performing a nucleic acid amplification procedure with the reaction mixture formed in step (5), the nucleic acid amplification resulting in the enzymatic synthesis of amplification products, each of the amplification products comprising (i) a target sequence contained in the nucleic acid or (ii) the complement of the target sequence;(7) selectively hybridizing a probe having a label to the target sequence or it complement of an amplification product that is one of the amplification products synthesized in step (6), thereby forming a hybrid in a mixture that comprises the probe and the amplification product; and(8) in the mixture, detecting [the label as an indication of] the formation of the hybrid in the mixture is an indication of the presence of the nucleic acid in the specimen. 18. The method of claim 17, wherein step (6) is performed in a temperature-controlled chamber of a first incubator, and wherein step (7) is performed in a temperature-controlled chamber of a second incubator, the first and second incubators being in a spaced-apart relationship. 19. The method of claim 17 further comprising, prior to step (A), a step of loading the specimen tube onto a specimen tube rack. 20. The method of claim 17 further comprising, prior to step (A), a step of providing to the analyzer all reagents required to perform a nucleic acid amplification, wherein the specimen tube and the reagents required to perform a nucleic acid amplification are separately provided to the analyzer. 21. The method of claim 17, wherein the receptacle is a cylindrical tube. 22. The method of claim 17, wherein the receptacle is one of a plurality of integrally formed receptacles. 23. The method of claim 22, wherein the integrally formed receptacles are arranged in a linear array. 24. The method of claim 17, wherein the nucleic acid is immobilized on the solid support with a capture probe in step (2). 25. The method of claim 17, wherein the automated process further comprises performing within the housing of the analyzer a step of washing the solid support one or more times after step (4) and prior to step (5). 26. The method of claim 17, wherein the fluid aspirator comprises an aspirator tube in frictional engagement with a contamination-limiting element configured to permit the passage of the fluid component of the specimen therethrough, and wherein the contamination-limiting element contacts the fluid component of the specimen in step (4). 27. The method of claim 26, wherein the contamination-limiting element is a tiplet. 28. The method of claim 17, wherein each of the amplification products consists of a nucleic acid sequence having the sequence contained in the nucleic acid or its complement. 29. The method of claim 17, wherein the amplification products include nucleic acid sequences having a sequence that is the same as the target sequence and nucleic acid sequences having a sequence that is the complement of the target sequence. 30. The method of claim 17, wherein step (6) is carried out in a temperature-controlled incubator maintained at a substantially uniform temperature. 31. The method of claim 17, wherein the label is a fluorescent or chemiluminescent label. 32. The method of claim 17, wherein the housing remains closed during the automated process. 33. The method of claim 17, wherein the automated process is performed at multiple locations of a processing deck contained within the housing. 34. The method of claim 17, wherein the hybrid is in solution.
Copyright KISTI. All Rights Reserved.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.