초록

The present invention provides compositions including antibodies that specifically bind at least one siderophore receptor polypeptide from a gram positive microbe. The present invention also provides methods of making and methods of using such compositions.

대표청구항 ▼

1. An antibody composition that specifically binds at least one siderophore receptor polypeptide (SRP) of a gram positive microbe, the composition prepared by a method comprising: collecting a body fluid from an animal that comprises antibody that specifically binds to at least one SRP of a gram pos

1. An antibody composition that specifically binds at least one siderophore receptor polypeptide (SRP) of a gram positive microbe, the composition prepared by a method comprising: collecting a body fluid from an animal that comprises antibody that specifically binds to at least one SRP of a gram positive microbe, wherein the animal has been administered an effective amount of a composition comprising at least two siderophore receptor polypeptides (SRPs) isolated from a gram positive microbe. 2. The antibody composition of claim 1 further comprising a pharmaceutically acceptable carrier. 3. The antibody composition of claim 1 wherein the gram positive microbe is an enteropathogen. 4. The antibody composition of claim 1 wherein the gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 5. The antibody composition of claim 4 wherein the member of the family Micrococcaceae is Staphylococcus spp. 6. The antibody composition of claim 4 wherein the member of the family Deinococcaceae is Streptococcus spp. 7. The antibody composition of claim 6 wherein the Streptococcus spp. is S. agalactiae, S. uberis, S. bovis, S. equi, S. zooepidemicus, or S. dysgalatiae. 8. The antibody composition of claim 4 wherein the member of the family Bacillaceae is Bacillus spp. 9. The antibody composition of claim 4 wherein the member of the family Clostridiaceae is Clostridium spp. 10. The antibody composition of claim 4 wherein the member of the family Corynebateriaceae is Corynebacterium spp. 11. The antibody composition of claim 4 wherein the member of the family Erysipelotrichidae is Erysipelothrix spp. 12. The antibody composition of claim 4 wherein the member of the family Listeriaceae is Listeria spp. 13. The antibody composition of claim 4 wherein the member of the family Mycobateriaceae is Mycobacterium spp. 14. The antibody composition of claim 1 wherein the composition administered to the animal comprises at least two SRPs having molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 15. The antibody composition of claim 14 wherein the gram positive microbe comprises a Staphylococcus spp., and wherein the at least two SRPs have a molecular weight of between about 88 kDa and about 92 kDa, between about 82 kDa and about 86 kDa, between about 70 kDa and about 74 kDa, or between about 64 kDa and about 68 kDa, wherein the molecular weight is determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 16. The antibody composition of claim 1 wherein the composition administered to the animal further comprises at least one porin. 17. The antibody composition of claim 16 wherein the at least one porin comprises a porin having molecular weight of about 30 kDa or about 43 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 18. The antibody composition of claim 16 wherein the at least one porin comprises two or more porins. 19. The antibody composition of claim 1 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a bisontine, a cervine, or a companion animal. 20. The antibody composition of claim 1 comprising antibody that specifically binds to an SRP present on the surface of a second gram positive microbe. 21. The antibody composition of claim 20 wherein the second gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 22. The antibody composition of claim 1 wherein the body fluid comprises serum, plasma, blood, milk, or colostrum. 23. The antibody composition of claim 1 wherein the antibody that specifically binds to at least one SRP comprises an antibody fragment. 24. The antibody composition of claim 23 wherein the antibody fragment comprises an scFv, a Fab, a F(ab′)2 or a Fv. 25. An antibody composition that specifically binds at least one siderophore receptor polypeptide (SRP) of a gram positive microbe, the composition prepared by a method comprising: collecting a body fluid from an animal that comprises antibody that specifically binds to at least one SRP of a gram positive microbe, wherein the animal has been administered a genetically modified gram positive whole cell that expresses at least two siderophore receptor polypeptides (SRPs). 26. The antibody composition of claim 25 further comprising a pharmaceutically acceptable carrier. 27. The antibody composition of claim 25 wherein the genetically modified gram positive whole cell is an enteropathogen. 28. The antibody composition of claim 25 wherein the genetically modified gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 29. The antibody composition of claim 28 wherein the member of the family Micrococcaceae is Staphylococcus spp. 30. The antibody composition of claim 28 wherein the member of the family Deinococcaceae is Streptococcus spp. 31. The antibody composition of claim 30 wherein the Streptococcus spp. is S. agalactiae, S. uberis, S. bovis, S. equi, S. zooepidemicus, or S. dysgalatiae. 32. The antibody composition of claim 28 wherein the member of the family Bacillaceae is Bacillus spp. 33. The antibody composition of claim 28 wherein the member of the family Clostridiaceae is Clostridium spp. 34. The antibody composition of claim 28 wherein the member of the family Corynebateriaceae is Corynebacterium spp. 35. The antibody composition of claim 28 wherein the member of the family Erysipelotrichidae is Erysipelothrix spp. 36. The antibody composition of claim 28 wherein the member of the family Listeriaceae is Listeria spp. 37. The antibody composition of claim 28 wherein the member of the family Mycobateriaceae is Mycobacterium spp. 38. The antibody composition of claim 25 wherein the genetically modified gram positive whole cell administered to the animal comprises at least two SRPs having molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 39. The antibody composition of claim 38 wherein the genetically modified gram positive whole cell comprises a Staphylococcus spp., and wherein the at least two SRPs have a molecular weight of between about 88 kDa and about 92 kDa, between about 82 kDa and about 86 kDa, between about 70 kDa and about 74 kDa, or between about 64 kDa and about 68 kDa, wherein the molecular weight is determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 40. The antibody composition of claim 25 wherein the genetically modified gram positive whole cell administered to the animal further expresses at least one porin. 41. The antibody composition of claim 40 wherein the at least one porin comprises a porin having molecular weight of about 30 kDa or about 43 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 42. The antibody composition of claim 40 wherein the at least one porin comprises two or more porins. 43. The antibody composition of claim 25 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a bisontine, a cervine, or a companion animal. 44. The antibody composition of claim 25 comprising antibody that specifically binds to an SRP present on the surface of a second gram positive microbe. 45. The antibody composition of claim 44 wherein the second gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 46. The antibody composition of claim 25 wherein the body fluid comprises serum, plasma, blood, milk, or colostrum. 47. The antibody composition of claim 25 wherein the antibody that specifically binds to at least one SRP comprises an antibody fragment. 48. The antibody composition of claim 47 wherein the antibody fragment comprises an scFv, a Fab, a F(ab′)2 or a Fv. 49. An isolated composition comprising: antibody that specifically binds to at least two siderophore receptor polypeptides (SRPs) from a gram positive microbe. 50. The antibody composition of claim 49 further comprising a pharmaceutically acceptable carrier. 51. The antibody composition of claim 49 wherein the gram positive microbe is an enteropathogen. 52. The antibody composition of claim 49 wherein the genetically modified gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 53. The antibody composition of claim 52 wherein the member of the family Micrococcaceae is Staphylococcus spp. 54. The antibody composition of claim 52 wherein the member of the family Deinococcaceae is Streptococcus spp. 55. The antibody composition of claim 54 wherein the Streptococcus spp. is S. agalactiae, S. uberis, S. bovis, S. equi, S. zooepidemicus, or S. dysgalatiae. 56. The antibody composition of claim 52 wherein the member of the family Bacillaceae is Bacillus spp. 57. The antibody composition of claim 52 wherein the member of the family Clostridiaceae is Clostridium spp. 58. The antibody composition of claim 52 wherein the member of the family Corynebateriaceae is Corynebacterium spp. 59. The antibody composition of claim 52 wherein the member of the family Erysipelotrichidae is Erysipelothrix spp. 60. The antibody composition of claim 52 wherein the member of the family Listeriaceae is Listeria spp. 61. The antibody composition of claim 52 wherein the member of the family Mycobateriaceae is Mycobacterium spp. 62. The antibody composition of claim 49 wherein the composition administered to the animal comprises at least two SRPs having molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 63. The antibody composition of claim 62 wherein the gram positive microbe comprises a Staphylococcus spp., and wherein the at least two SRPs have a molecular weight of between about 88 kDa and about 92 kDa, between about 82 kDa and about 86 kDa, between about 70 kDa and about 74 kDa, or between about 64 kDa and about 68 kDa, wherein the molecular weight is determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 64. The antibody composition of claim 49 further comprising antibody that specifically binds to at least one porin. 65. The antibody composition of claim 64 wherein the at least one porin comprises a porin having molecular weight of about 30 kDa or about 43 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 66. The antibody composition of claim 64 wherein the at least one porin comprises two or more porins. 67. The antibody composition of claim 49 comprising antibody that specifically binds to an SRP present on the surface of a second gram positive microbe. 68. The antibody composition of claim 67 wherein the second gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 69. The antibody composition of claim 49 comprising a body fluid from an animal. 70. The antibody composition of claim 69 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a bisontine, a cervine, or a companion animal. 71. The antibody composition of claim 69 wherein the body fluid comprises serum, plasma, blood, milk, or colostrum. 72. The antibody composition of claim 49 wherein the antibody that specifically binds to at least one SRP comprises an antibody fragment. 73. The antibody composition of claim 72 wherein the antibody fragment comprises an scFv, a Fab, a F(ab′)2 or a Fv. 74. A composition comprising: a body fluid from an animal, or a fraction thereof, comprising antibody that specifically binds to at least two siderophore receptor polypeptides (SRPs) from a gram positive microbe; anda pharmaceutically acceptable excipient. 75. The composition of claim 74 wherein the body fluid comprises serum, plasma, blood, milk, or colostrum. 76. The composition of claim 74 wherein the animal is an avian, a bovine, a caprine, a porcine, an ovine, a bisontine, a cervine, or a companion animal. 77. The antibody composition of claim 74 wherein the gram positive microbe is an enteropathogen. 78. The antibody composition of claim 74 wherein the gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae. 79. The antibody composition of claim 78 wherein the member of the family Micrococcaceae is Staphylococcus spp. 80. The antibody composition of claim 78 wherein the member of the family Deinococcaceae is Streptococcus spp. 81. The antibody composition of claim 80 wherein the Streptococcus spp. is S. agalactiae, S. uberis, S. bovis, S. equi, S. zooepidemicus, or S. dysgalatiae. 82. The antibody composition of claim 78 wherein the member of the family Bacillaceae is Bacillus spp. 83. The antibody composition of claim 78 wherein the member of the family Clostridiaceae is Clostridium spp. 84. The antibody composition of claim 78 wherein the member of the family Corynebateriaceae is Corynebacterium spp. 85. The antibody composition of claim 78 wherein the member of the family Erysipelotrichidae is Erysipelothrix spp. 86. The antibody composition of claim 78 wherein the member of the family Listeriaceae is Listeria spp. 87. The antibody composition of claim 78 wherein the member of the family Mycobateriaceae is Mycobacterium spp. 88. The antibody composition of claim 74 wherein the composition administered to the animal comprises at least two SRPs having molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 89. The antibody composition of claim 88 wherein the gram positive microbe comprises a Staphylococcus spp., and wherein the at least two SRPs have a molecular weight of between about 88 kDa and about 92 kDa, between about 82 kDa and about 86 kDa, between about 70 kDa and about 74 kDa, or between about 64 kDa and about 68 kDa, wherein the molecular weight is determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 90. The antibody composition of claim 74 further comprising antibody that specifically binds to at least one porin. 91. The antibody composition of claim 90 wherein the at least one porin comprises a porin having molecular weight of about 30 kDa or about 43 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 92. The antibody composition of claim 90 wherein the at least one porin comprises two or more porins. 93. The antibody composition of claim 74 comprising antibody that specifically binds to an SRP present on the surface of a second gram positive microbe. 94. The antibody composition of claim 89 wherein the second gram positive microbe is a member of the family Micrococcaceae, family Deinococcaceae, family Bacillaceae, family Clostridiaceae, family Corynebateriaceae, family Erysipelotrichidae, family Listeriaceae, or family Mycobateriaceae.