Process of separating components of a fermentation broth
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-007/18
C12P-007/02
C12P-007/00
출원번호
US-0793623
(2010-06-03)
등록번호
US-8597918
(2013-12-03)
발명자
/ 주소
Clark, Warren
Japs, Michael
Burk, Mark J.
출원인 / 주소
Genomatica, Inc.
대리인 / 주소
Jones Day
인용정보
피인용 횟수 :
5인용 특허 :
103
초록▼
A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for produci
A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.
대표청구항▼
1. A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth comprising separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, wherein said step of separating said liquid fraction comprises microfiltration;removing water from said liquid fraction, wh
1. A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth comprising separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, wherein said step of separating said liquid fraction comprises microfiltration;removing water from said liquid fraction, wherein removing water is accomplished by evaporation with an evaporator system comprising one or more effects and a mechanical vapor recompressor;removing salts from said liquid fraction, wherein salts are removed by nanofiltration and by ion exchange; andpurifying 1,4-BDO. 2. The process of claim 1, wherein microfiltration comprises filtering through a membrane having pore sizes from about 0.05 microns to about 5.0 microns. 3. The process of claim 1, wherein said step of separating said liquid fraction further comprises ultrafiltration. 4. The process of claim 3, wherein ultrafiltration comprises filtering through a membrane having pore sizes from about 0.005 to about 0.1 microns. 5. The process of claim 1, wherein said evaporator system comprises a double- or triple-effect evaporator. 6. The process of claim 1, wherein said evaporator system comprises an evaporator selected from the group consisting of a falling film evaporator, a short path falling film evaporator, a forced circulation evaporator, a plate evaporator, a circulation evaporator, a fluidized bed evaporator, a rising film evaporator, a counterflow-trickle evaporator, a stirrer evaporator, and a spiral tube evaporator. 7. The process of claim 1, wherein said evaporator system comprises a vacuum. 8. The process of claim 1, wherein substantially all of the salts are removed prior to removal of water. 9. The process of claim 1, wherein substantially all of the salts are removed after removal of a portion of water. 10. The process of claim 1, wherein salts are removed after removal of substantially all of the water. 11. The process of claim 1, wherein nanofiltration comprises filtering through a membrane having a pore size range from about 0.0005 to about 0.005 microns. 12. The process of claim 1, wherein said solid fraction comprising cells is recycled into said fermentation broth. 13. The process of claim 1, wherein said removed water is recycled into said fermentation broth. 14. The process of claim 1, wherein said removed salts are recycled into said fermentation broth. 15. The process of claim 1, wherein said step of purifying 1,4-BDO comprises distillation. 16. A process for producing 1,4-BDO comprising: culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO, said 1,4-BDO producing microorganism comprising a microorganism having a 1,4-BDO pathway comprising one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions;isolating 1,4-BDO by a process according to claim 1. 17. The process of claim 1, wherein removing salts by ion exchange comprises removing salts by cation exchange followed by anion exchange.
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