초록 ▼

The invention relates to a method for producing a multi-layer collagen scaffold. The method generally comprises the steps of: preparing a first suspension of collagen and freezing or lyophilizing the suspension to provide a first layer; optionally preparing a further suspension of collagen and addin

The invention relates to a method for producing a multi-layer collagen scaffold. The method generally comprises the steps of: preparing a first suspension of collagen and freezing or lyophilizing the suspension to provide a first layer; optionally preparing a further suspension of collagen and adding the further suspension onto the layer formed in the previous step to form a further layer, and freezing or lyophilizing the layers, wherein when the layer formed in the previous step is formed by lyophilization the lyophilized layer is re-hydrated prior to addition of the next layer; optionally, repeating the aforementioned step to form one or more further layers; and preparing a final suspension of collagen and pouring the final suspension onto the uppermost layer to form a final layer, and freeze-drying the layers to form the multilayer collagen composite scaffold.

대표청구항 ▼

1. A process for producing a multi-layer scaffold in which each layer has a porous structure, the process comprising an iterative layering technique in which each layer is independently formed by freezing, or lyophilisation followed by rehydration with an acidic solvent, prior to addition of a follo

1. A process for producing a multi-layer scaffold in which each layer has a porous structure, the process comprising an iterative layering technique in which each layer is independently formed by freezing, or lyophilisation followed by rehydration with an acidic solvent, prior to addition of a following layer, wherein the final layer is formed by lyophilisation. 2. A method as claimed in claim 1 in which all layers in the scaffold are formed by lyophilisation. 3. A method according to claim 1 for producing a three layer collagen scaffold, the method comprising the steps of: preparing a first homogenous suspension of collagen and lyophilising the suspension to provide a first layer;rehydrating the formed first layer with an acidic solvent;preparing a second homogenous suspension of collagen and pouring the second homogenous suspension onto the re-hydrated first layer to form a two-layered composite, and lyophilising the two-layer composite;re-hydrating the two-layer composite; andpreparing a third homogenous suspension of collagen and pouring the third homogenous suspension onto the two-layer composite to form a three-layer composite, and lyophilising the three-layer composite to form the three-layer collagen scaffold. 4. A method according to claim 1 for preparing a three layer collagen scaffold suitable for use as an osteochondral repair scaffold, the method comprising the steps of: preparing a first suspension of Type I collagen and HA in the form of a slurry and freezing or lyophilising the slurry to provide a first layer;when the first layer is formed by freeze-drying, rehydrating the formed first layer with an acidic solvent;preparing a second suspension of Type I collagen, Type II collagen, and HA in the form of a slurry and adding the slurry onto the first layer to form a two-layer composite, and freezing or lyophilising the two-layer composite;when the second layer is formed by freeze-drying, rehydrating the second layer with an acidic solvent;preparing a third suspension of Type I and Type II collagen and optionally a polymer and/or a biologic, and adding the third suspension onto the two-layer composite to form a three-layer composite, and lyophilising the three layer composite to form the three-layer collagen scaffold; andoptionally, crosslinking one or more of the layers of the scaffold.