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특허 상세정보

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내보내기 페이스북 공유 카카오톡 공유

Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12N-005/00    C12N-015/00   
미국특허분류(USC) 435/387; 435/395; 435/455
출원번호 US-0170219 (2008-07-09)
등록번호 US-8637311 (2014-01-28)
발명자 / 주소
  • Mandalam, Ramkumar
  • Xu, Chunhui
  • Gold, Joseph D.
  • Carpenter, Melissa K.
출원인 / 주소
  • Asterias Biotherapeutics, Inc.
대리인 / 주소
    Law Office of Salvatore Arrigo and Scott Lee, LLP
인용정보 피인용 횟수 : 7  인용 특허 : 47
초록
▼

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being pr...

대표
청구항
▼

1. A method of genetically modifying human embryonic stem (“hES”) cells, comprising: a. culturing hES cells essentially feeder free on an extracellular matrix in a non-conditioned nutrient media comprising fibroblast growth factor (FGF); andb. introducing a nucleic acid into the hES cells of a) thereby genetically modifying the hES cells. 2. The method of claim 1, wherein the extracellular matrix comprises at least one of fibronectin, laminin, proteoglycan, entactin, and heparin sulfate. 3. The method of claim 1, wherein the extracellular matrix comprise...

이 특허에 인용된 특허 (47)

  1. Seilhamer Jeffrey J. ; Scott Randal W., Comparative gene transcript analysis.
  2. Xu, Chunhui; Gold, Joseph D., Conditioned media for propagating human pluripotent stem cells.
  3. Wandrey Christian,DEX ; Biselli Manfred,DEX ; Schroder Bernd,DEX ; Schmoll Hans-Joachim,DEX, Culturing cells on macroporous glass carriers coated with gelatin, extracellular matrix protein and stromal cells.
  4. Knowles Barbara B. ; Rothstein Jay L. ; Johnson Dabney ; Solter Davor,DEX, Developmental embryonic mouse cDNA libraries.
  5. Keller Gordon M. ; Kennedy Marion ; Choi Kyunghee ; Firpo Meri T., Embryonic cell populations and methods to isolate such populations.
  6. Reubinoff, Benjamin Eithan; Pera, Martin Frederick; Fong, Chui-Yee; Trounson, Alan Osborne; Bongso, Ariffeen, Embryonic stem cells.
  7. Verfaillie Catherine M. ; McGlave Philip B. ; Miller Jeffrey S., Ex vivo culture of stem cells.
  8. Kaufman Dan S. ; Thomson James A., Hematopoietic differentiation of human pluripotent embryonic stem cells.
  9. Lakshmi Rambhatla ; Melissa K. Carpenter, Hepatocyte lineage cells derived from pluripotent stem cells.
  10. Rambhatla, Lakshmi; Carpenter, Melissa K., Hepatocyte lineage cells derived from pluripotent stem cells.
  11. Rambhatla,Lakshmi; Carpenter,Melissa K., Hepatocytes for therapy and drug screening made from embryonic stem cells.
  12. Carpenter Melissa, Human CNS neural stem cells.
  13. Gearhart John D. ; Shamblott Michael Joseph, Human embryonic germ cell line and methods of use.
  14. Gearhart John D. ; Shamblott Michael Joseph, Human embryonic pluripotent germ cells.
  15. Gold,Joseph D.; Carpenter,Melissa K.; Inokuma,Margaret S.; Xu,Chunhui, Human embryonic stem cells having genetic modifications.
  16. Au-Young Janice, Human stem cell antigens.
  17. Anderson David J. (Altadena CA) Stemple Derek L. (Newton MA), Immoralized neural crest stem cells and methods of making.
  18. Johe Karl K., In vitro generation of differentiated neurons from cultures of mammalian multipotential CNS stem cells.
  19. Weiss Samuel,CAX ; Reynolds Brent,CAX, In vitro induction of dopaminergic cells.
  20. Williams Robert L. (Warrndyte AUX) Gough Nicholas M. (North Balwyn AUX) Hilton Douglas J. (Warrandyte AUX), In vitro propagation of embryonic stem cells.
  21. Carpenter, Melissa K., Making neural cells for human therapy or drug screening from human embryonic stem cells.
  22. Villeponteau Bryant (San Carlos CA) Feng Junli (San Carlos CA) Funk Walter (Union City CA) Andrews William H. (Richmond CA), Mammalian telomerase.
  23. Xu,Chunhui; Li,Yan; Mandalam,Ramkumar, Medium for growing human embryonic stem cells.
  24. Ponting Ian L. O. (Woodland Hills CA), Medium for long-term proliferation and development of cells.
  25. Fisher Paul B. (Scarsdale NY) Jiang Hongping (New York NY), Method for generating a subtracted cDNA library and uses of the generated library.
  26. Gay David A. (San Diego CA), Method of isolating a lineage specific stem cell in vitro.
  27. Fei Rui G. (Seattle WA) Heimfeld Shelly (Woodinville WA) Minshall Billy W. (Mill Creek WA) Berenson Ronald J. (Mercer Island WA), Methods and device for culturing human hematopoietic cells and their precursors.
  28. Bodnar, Andrea G.; Chiu, Choy-Pik; Gold, Joseph D.; Inokuma, Margaret; Murai, James T.; West, Michael D., Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture.
  29. Gold, Joseph D.; Lebkowski, Jane S., Methods for providing differentiated stem cells.
  30. Bradley Allan (Houston TX) Davis Ann (Houston TX) Hasty Paul (Houston TX), Methods for the genetic modification of endogenous genes in animal cells by homologous recombination.
  31. Hogan Brigid L. M. (Brentwood TN), Pluripotential embryonic stem cells and methods of making same.
  32. Capecchi Mario R. (Salt Lake City UT) Thomas Kirk R. (Salt Lake City UT), Positive-negative selection methods and vectors.
  33. Conover Joanne (Tarrytown) Yancopoulos George D. (Tarrytown), Prevention of ES cell differentiation by ciliary neurotrophic factor.
  34. Thomson James A., Primate embryonic stem cells.
  35. Thomson James A., Primate embryonic stem cells.
  36. Boom Willem R. (Amsterdam NLX) Adriaanse Henritte M. A. (Arnhem NLX) Kievits Tim (The Hague NLX) Lens Peter F. (Amsterdam NLX), Process for isolating nucleic acid.
  37. Rambhatla,Lakshmi; Carpenter,Melissa K., Process for making hepatocytes from pluripotent stem cells.
  38. Mandalam,Ramkumar; Faouzi,Saadia; Nadeau,Isabelle; Pfendler Bonham,Kristina; Rao,Namitha; Carpenter,Melissa K.; Rambhatla,Lakshmi; Chiu,Choy Pik, Protocols for making hepatocytes from embryonic stem cells.
  39. Weinrich Scott L. ; Atkinson ; III Edward M. ; Lichtsteiner Serge P. ; Vasserot Alain P. ; Pruzan Ronald A. ; Kealey James T., Purified telomerose.
  40. Kleinman Hynda K. (Bethesda MD) Martin George R. (Bethesda MD), Reconstituted basement membrane complex with biological activity.
  41. McWhir, Jim; Gold, Joseph D.; Schiff, J. Michael, Selective antibody targeting of undifferentiated stem cells.
  42. Thomson,James A, Serum free cultivation of primate embryonic stem cells.
  43. Lee Jar-How (Los Angeles CA) Blair Lindley (Los Angeles CA) Horwitz Arnold (Los Angeles CA) Koduri Raju K. (Los Angeles CA), Shortened phosphoglycerate kinase promoter.
  44. Gerson Stanton L. (Pepper Pike OH) Caplan Arnold I. (Cleveland Heights OH) Haynesworth Stephen E. (Cleveland Heights OH), Transduced mesenchymal stem cells.
  45. Wheeler Matthew B., Transgenic swine compositions and methods.
  46. Wheeler Matthew B. (Tolono IL), Transgenic swine compositions and methods.
  47. Carpenter,Melissa K.; Inokuma,Margaret S.; Xu,Chunhui, Use of human embryonic stem cells for drug screening and toxicity testing.

이 특허를 인용한 특허 (7)

  1. Amit, Michal; Itskovitz-Eldor, Joseph, Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state.
  2. Amit, Michal; Itskovitz-Eldor, Joseph, Media for culturing stem cells.
  3. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor.
  4. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor.
  5. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor β1 and basic fibroblast growth factor.
  6. Amit, Michal; Itskovitz-Eldor, Joseph, Methods of expanding embryonic stem cells in a suspension culture.
  7. Amit, Michal; Itskovitz-Eldor, Joseph, Methods of expanding embryonic stem cells in a suspension culture.

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