초록 ▼

An improved method and apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution with a broad range of anti-infective and therapeutic applications. The resulting electrolyzed saline solution exhibits a marked lack of toxicity upon intravenous, aspired, oral or topical a

An improved method and apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution with a broad range of anti-infective and therapeutic applications. The resulting electrolyzed saline solution exhibits a marked lack of toxicity upon intravenous, aspired, oral or topical application in mammals for therapeutic applications providing a broad platform, including topical disinfection, antimicrobial application, wound treatment, oxidative stress reduction and enhancement of immune function to better detect malfunctioning cells.

대표청구항 ▼

1. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal, comprising: a. preparing a saline solution having a saline concentration of at least abou

1. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal, comprising: a. preparing a saline solution having a saline concentration of at least about 0.15%,b. inserting within the saline solution an inert anode and a spaced apart corresponding inert cathode associated with a power source,c. regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates and regulate relative concentrations of resulting components during electrolysis,d. circulating the saline solution to maintain a flow of the saline solution between the anode and cathode, ande. applying an effective voltage potential less than about thirty volts between the cathode and the anode sufficient to produce a target mixture of chemically reduced and oxidized species including Hypochlorous acid (HOCl), Hypochlorites (OCl−, NaClO), dissolved Oxygen (O2), Chlorine (Cl2) between 1 to 200 ppm and Hydrogen (H2) gas, Hydrogen Peroxide (H2O2), Hydrogen ions (H+), Hypochloride (ClO) and corresponding amounts of Superoxides (*O2−, HO2), Ozone (O3) from 1 to 50 ppm, Activated Hydrogen ions (H−), Chloride ions (Cl−), Hydroxides (NaOH, OH−), Singlet Oxygen (*O2) and other forms of Reactive Oxygen Species (ROS) (*OCl, *HO−), and wherein total ROS is between about 0.05 and about 50 ppm, utilizing at least one of electron and proton donation, ion and dissolved-gas transport, the temperature, anode and cathode spacing, saline solution circulation rate, and effective voltage combination to achieve desired electrolysis efficiencies and stable specie compositions containing stable ROS compounds while preventing production of chlorates,f. mirroring the target mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluorospectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein thereby producing the electrolyzed saline solution; andg. administering the electrolyzed saline solution balanced mixture to a human or warm-blooded animal for therapeutic use to attack infective microbes and enhance the ability of the immune system to recognize and destroy damaged or malfunctioning cells. 2. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm blooded-animal according to claim 1, wherein the electrolyzed saline solution is administered by injection, oral or anal ingestion, applied topically, used as a bath, applied in a wound dressing, or inhaled in atomized form. 3. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal according to claim 1, wherein the electrolyzed saline solution is placed in container means fabricated from a biologically compatible material. 4. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded-animal according to claim 1, wherein the anode is made of a base metal selected from the group consisting of platinum, niobium, titanium or any metal compatible with platinum bonding and is coated with an outer layer of platinum bonded to the base metal. 5. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal according to claim 4, wherein the anode has a cylindrical, or flat (planar) shaped structure. 6. A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal according to claim 1, wherein the cathode is positioned coaxially or in parallel in relation to the anode. 7. A method for using an electrolyzed saline solution for treating a human or warm-blooded animal, comprising: electrolyzing a saline solution having a salt concentration between about 0.15% and about 1.0% by weight using a voltage between about 0 V and about 30 V across an inert anode and a spaced apart inert cathode thereby generating a target mixture of chemically reduced and oxidized molecules within the saline solution;mirroring the target mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluorospectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein thereby producing the electrolyzed saline solution; andadministering the electrolyzed saline solution to treat a human or warm-blooded animal by attacking infective microbes and enhancing an immune system. 8. The method according to claim 7, further comprising regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates. 9. The method according to claim 7, further comprising circulating the saline solution to maintain a flow of the saline solution between the anode and the cathode. 10. The method according to claim 7, wherein the target mixture of chemically reduced and oxidized species includes at least one of hypochlorous acid, hypochlorites, dissolved oxygen chlorine, hydrogen gases, hydrogen peroxide, hydrogen, hypochloride and corresponding amounts of superoxides, ozone, activated hydrogen, chloride ions, hydroxides, singlet oxygen, and other forms of reactive oxygen species. 11. The method according to claim 10, wherein the total combination of hypochlorous acid, hypochlorites, and dissolved oxygen chlorine is at a concentration between 1 to 200 ppm, wherein the total combination of hydrogen gases, hydrogen peroxide, hydrogen, hypochloride, superoxides, and ozone is at a concentration from 1 to 50 ppm. 12. The method according to claim 11, wherein total reactive oxygen species in the mixture is between 0.05 to 50 ppm. 13. The method according to claim 7, wherein the electrolyzed saline solution is administered by injection, oral or anal ingestion, applied topically, used as a bath, applied in a wound dressing, or inhaled in atomized form. 14. The method according to claim 7, wherein the electrolyzed saline solution is placed in container fabricated from a biologically compatible material. 15. The method according to claim 7, wherein the anode is made of a base metal selected from the group consisting of platinum, niobium, titanium or any metal compatible with platinum bonding and is coated with an outer layer of platinum bonded to the base metal. 16. The method according to claim 15, wherein the anode has a cylindrical, or planar shaped structure. 17. The method according to claim 7, wherein the cathode is positioned coaxially or in parallel in relation to the anode.