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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0664953 (2012-10-31) |
등록번호 | US-8691594 (2014-04-08) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 0 인용 특허 : 462 |
A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional
A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.
1. A method of making a bead array comprising: a) contacting a substrate having a planar surface with a suspension comprising populations of several differently-encoded beads having diameters of up to 10 microns, wherein each population of differently-encoded beads has a different biomolecule attach
1. A method of making a bead array comprising: a) contacting a substrate having a planar surface with a suspension comprising populations of several differently-encoded beads having diameters of up to 10 microns, wherein each population of differently-encoded beads has a different biomolecule attached thereto and wherein at least a portion of the biomolecules are bound to an analyte of interest; and b) applying energy to said substrate or said suspension, or both, such that the populations of differently-encoded beads form an ordered array on the planar surface and the beads are at pre-determined distances from each other. 2. The method according to claim 1 wherein the biomolecule is a protein or an oligonucleotide. 3. The method according to claim 1 wherein the beads are in a planar crystalline arrangement. 4. The method according to claim 1 wherein the beads are in a hexagonally crystalline configuration. 5. The method according to claim 1 wherein the beads are in a bubble raft arrangement. 6. The method according to claim 1 wherein the beads are encoded using oligonucleotides. 7. The method according to claim 1 wherein the beads are binary encoded. 8. The method according to claim 1 wherein the substrate has a patterned surface. 9. The method according to claim 1 wherein the ordered array of beads comprises subarrays of beads. 10. The method according to claim 1 wherein the beads have a 2-10 micron diameter. 11. The method according to claim 1 wherein the beads range in size from approximately 100 Angstroms to 10 microns. 12. The method according to claim 1 wherein the energy is a magnetic field. 13. The method according to claim 1 wherein the energy is electrical energy. 14. The method according to claim 1 wherein the beads are magnetic. 15. A method of making a bead array comprising: a) contacting a substrate having a planar surface with a suspension comprising populations of several differently-encoded beads having diameters of up to 10 microns, wherein each population of differently-encoded beads has a different biomolecule attached thereto, such that the encoding of each bead corresponds uniquely to the biomolecule attached thereto; and b) applying energy to said substrate or said suspension, or both, such that the populations of differently-encoded beads form an ordered array on the planar surface and the beads are at pre-determined distances from each other. 16. The method according to claim 15 wherein the beads are immobilized to the substrate following step b). 17. The method according to claim 16 wherein the beads are immobilized by anchoring to the substrate. 18. The method according to claim 16 wherein the beads are immobilized by chemical attachment, heterobifunctional cross-linking agents, or physical attraction to the substrate. 19. The method according to claim 18 wherein physical attraction is through van der Waals forces. 20. The method according to claim 15 wherein the biomolecule is a protein or an oligonucleotide.
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