Quantitative trait loci associated with soybean cyst nematode resistance and methods of their use
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-001/00
A01H-001/04
출원번호
US-0896864
(2010-10-02)
등록번호
US-8692064
(2014-04-08)
발명자
/ 주소
Nguyen, Henry T.
Sleper, David A.
Shannon, James G.
Vuong, Tri D.
Wu, Xiaolei
출원인 / 주소
The Curators of the University of Missouri
대리인 / 주소
Lathrop & Gage LLP
인용정보
피인용 횟수 :
0인용 특허 :
10
초록▼
Several QTLs that are genetically linked to resistance to soybean cyst nematode (SCN) are disclosed. These QTLs have been mapped to genomic regions on Chrs. 4, 8, 10, 11, 18, and 20 of soybean, G. max. Candidate genes underlying these QTLs as defined by the flanking markers, as well as genetic marke
Several QTLs that are genetically linked to resistance to soybean cyst nematode (SCN) are disclosed. These QTLs have been mapped to genomic regions on Chrs. 4, 8, 10, 11, 18, and 20 of soybean, G. max. Candidate genes underlying these QTLs as defined by the flanking markers, as well as genetic markers associated with these QTLs are also disclosed. These markers can be utilized for introgressing SCN resistance into non-resistant soybean germplasm. The unique resistance genes can be introduced into a non-resistant plant by marker-assisted selection (MAS) or by transgenic methods.
대표청구항▼
1. A method for generating a transgenic plant using a host plant, said transgenic plant being more resistant to soybean cyst nematode (SCN) when compared to the host plant, said method comprising a step of introducing at least one transgene into said host plant, said at least one transgene being loc
1. A method for generating a transgenic plant using a host plant, said transgenic plant being more resistant to soybean cyst nematode (SCN) when compared to the host plant, said method comprising a step of introducing at least one transgene into said host plant, said at least one transgene being located within the chromosomal region as defined by one QTL selected from the group consisting of SCN QTL 1-11. 2. A method for generating a new soybean cyst nematode (SCN) resistant plant by crossing two parental strains, said new plant being more resistant to SCN than at least one of the two parental strains, said method comprising a step of introducing a genomic region into said new plant through the crossing, said genomic region being the chromosomal region of claim 1 containing the transgene. 3. The method of claim 2, wherein the genomic region comprises less than 100,000 nucleotides. 4. The method of claim 2, wherein the genomic region comprises less than 10,000 nucleotides. 5. A method for generating a soybean plant, said method comprising (a) introgressing SCN resistance into an SCN sensitive soybean germplasm, (b) determining the presence or absence of a marker gene or a fragment thereof and a transgene, said marker gene being selected from the group consisting of the polynucleotide molecules of SEQ ID Nos. 1-22 and said transgene being the transgene of claim 1, and (c) allowing the germplasm generated in (a) to develop into a soybean plant resistant to soybean cyst nematode (SCN) if the marker and transgene are present, wherein step (a) precedes step (b). 6. The method of claim 5, wherein the soybean plant is generated through one or more crosses of two or more parental soybean plants. 7. The method of claim 5, wherein the presence or absence of the marker gene is determined by using DNA hybridization. 8. The method of claim 5, wherein the presence or absence of the marker gene is determined by using DNA polymerase chain reaction (PCR).
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이 특허에 인용된 특허 (10)
McCabe Dennis E. (Middleton WI) Martinell Brian J. (Madison WI), Apparatus for genetic transformation.
Sanford John C. (Geneva NY) Wolf Edward D. (Ithaca NY) Allen Nelson K. (Newfield NY), Method for transporting substances into living cells and tissues and apparatus therefor.
Vierling Richard A. ; Faghihi Jamal ; Ferris Virginia R. ; Ferris John M., Methods for conferring broad-based soybean cyst nematode resistance to a soybean line.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
Schilperoort Robbert A. (Leiden NLX) Hoekema Andreas (Leiden NLX), Process for the incorporation of foreign DNA into the genome of dicotyledonous plants.
Schilperoort Robbert A. (Anthonie Duycklaan 10c ; 2334 CD Leiden NLX) Hoekema Andreas (Boerhaavelaan 114 ; 2334 ET Leiden NLX) Hooykaas Paul J. J. (Condorstraat 126 ; 2317 AW Leiden NLX), Process for the incorporation of foreign dna into the genome of dicotyledonous plants.
Paszkowski Jerzy (Zurich CHX) Potrykus Ingo (Magden CHX) Hohn Barbara (Bottmingen NC CHX) Shillito Raymond D. (Chapel Hill NC) Hohn Thomas (Bottmingen CHX) Saul Michael W. (Zurich CHX) Mandak Vaclav , Transformation of hereditary material of plants.
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