Membrane-based lateral flow assay devices that utilize phosphorescent detection
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/558
G01N-033/53
G01N-033/543
출원번호
US-0017342
(2013-09-04)
등록번호
US-8703504
(2014-04-22)
발명자
/ 주소
Song, Xuedong
출원인 / 주소
Kimberly-Clark Worldwide, Inc.
대리인 / 주소
Dority & Manning, P.A.
인용정보
피인용 횟수 :
2인용 특허 :
301
초록▼
A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that backgroun
A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.
대표청구항▼
1. A method for detecting an analyte within a test sample, the method comprising: i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, the detection probes comprising a phosphorescent particle, the phosphorescent particle comprising
1. A method for detecting an analyte within a test sample, the method comprising: i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, the detection probes comprising a phosphorescent particle, the phosphorescent particle comprising a phosphorescent metal complex encapsulated within a barrier matrix comprising polymethylmethacrylate, the detection probes being conjugated with a specific binding member configured to bind with the analyte and form an analyte complex, wherein the porous membrane defines a conjugate pad, the conjugate pad including the detection probes; a detection zone positioned downstream of the conjugate pad within which is immobilized a capture reagent that is configured to bind to the analyte complex to generate a detection signal; and a calibration zone positioned downstream of the detection zone within which a capture reagent is immobilized, the capture reagent being capable of binding to uncaptured detection probes to generate a calibration signal;ii) contacting the lateral flow assay device with the test sample;iii) subjecting the detection zone to illumination pulses to generate the detection signal;iv) subjecting the calibration zone to illumination pulses to generate the calibration signal; andv) thereafter, measuring the intensity of the detection signal and the calibration signal, wherein the amount of the analyte within the test sample is proportional to a ratio of the intensity of the detection signal to the intensity of the calibration signal. 2. A method as in claim 1, wherein the metal complex comprises a metal selected from the group consisting of ruthenium, osmium, rhenium, iridium, rhodium, platinum, indium, palladium, molybdenum, technetium, copper, iron, chromium, tungsten, zinc, and combinations thereof. 3. A method as in claim 1, wherein the metal complex comprises a ligand selected from the group consisting of pyridine, pyrazine, isonicotinamide, imidazole, bipyridine, terpyridine, phenanthroline, dipyridophenazine, porphyrin, porphine, derivatives thereof, and combinations thereof. 4. A method as in claim 1, wherein the metal complex comprises a porphine complex. 5. A method as in claim 1, wherein the metal complex is selected from the group consisting of platinum(II) tetra-meso-fluorophenylporphine and palladium(II) tetra-meso-fluorophenylporphine. 6. A method as in claim 1, wherein the particle comprises a nanoparticle. 7. A method as in claim 6, wherein the nanoparticle has a diameter of from about 1 nanometer to about 10 microns. 8. A method as in claim 1, wherein the barrier matrix shields the encapsulated phosphorescent label from phosphorescence quenchers. 9. A method as in claim 1, wherein the specific binding member is selected from the group consisting of antigens, haptens, aptamers, primary or secondary antibodies, biotin, and combinations thereof. 10. The method as in claim 1, wherein the metal complex comprises platinum(II) tetra-meso-fluorophenylporphine. 11. The method as in claim 1, wherein the metal complex comprises palladium(II) tetra-meso-fluorophenylporphine. 12. The method as in claim 1, wherein the phosphorescent metal complex comprises a phosphorescent label that has a phosphorescent lifetime of greater than about 1 microsecond. 13. The method as in claim 1, wherein the phosphorescent metal complex comprises a phosphorescent label that has a Stokes shift of greater than about 100 nm. 14. The method as in claim 1, further comprising a sampling pad located upstream of the conjugate pad. 15. The method as in claim 1, wherein the capture reagent in the calibration zone is a polyelectrolyte capture reagent. 16. The method as in claim 15, wherein the polyelectrolyte capture reagent is a non-biological reagent. 17. The method as in claim 1, wherein the detection zone and the calibration zone are simultaneously subjected to one or more pulses of light. 18. The method as in claim 1, wherein the intensity of the detection signal and the intensity of the calibration signal are measured simultaneously. 19. The method as in claim 1, wherein the intensity of the detection signal is measured after about 1 to about 100 microseconds.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (301)
Monbouquette Harold G., Amperometric biosensors based on redox enzymes.
Moorman David R. (Indianapolis IN) Ledden David J. (Indianapolis IN) Webster David D. (Indianapolis IN) Heald Brian A. (Indianapolis IN), Analytical test apparatus with on board negative and positive control.
Miffitt Donald (Chelmsford MA) Poto Edward (Somerville NJ) Hovorka George (Bedminster NJ) Costaris Arthur (Hampton NJ), Apparatus and method for measuring a bodily constituent.
Liberti Paul A. (Churchville PA) Wang Yuzhou (Wayne PA) Tang Weixin (Devon PA) Feeley Brian P. (Easton PA) Gohel Dhanesh I. (Philadelphia PA), Apparatus and methods for magnetic separation featuring external magnetic means.
Van Gelder Ezra (Belmont CA) Liang Bee C. (Sunnyvale CA) Johnston Richard F. (Murphys CA) Loder ; Jr. Robert T. (Sunnyvale CA), Apparatus for measuring spatial distribution of fluorescence on a substrate.
Lawrence Paul J. (Campbell CA) Andreasen Terrence J. (San Jose CA) Shockey David R. (Cupertino CA), Assay for proline iminopeptidase and other hydrolytic activities.
Walling P. Douglas (Indianapolis IN) Bateson Joseph E. (Carmel IN) Kuhn Lance S. (Fishers IN) Ochs M. Luann (Fishers IN) Kost Kent M. (Fishers IN) Gerber Martin T. (Carmel IN) Pollmann Klaus H. (Neul, Assay method with enzyme electrode system.
Phillips Roger ; McGarraugh Geoffery ; Jurik Franklin A. ; Underwood Raymond D., Automatic initiation of a time interval for measuring glucose concentration in a sample of whole blood.
Ekins Roger P. (Department of Molecular Endocrinology University College and Middlesex School of Medicine Mortimer Street London W1N 8AA GBX) Chu Frederick W. (Department of Molecular Endocrinology U, Binding assay employing labelled reagent.
Everhart Dennis S. ; Grunze Michael,DEX ; Kaylor Rosann Marie ; Morhard Friderike Karolin Deseree,DEX, Biosensing devices which produce diffraction images.
Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jimmy D. (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir E. (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
Malmqvist Magnus (Upsala SEX) Karlsson Robert (Upsala SEX) Larsson Anita (Upsala SEX) Sjodal Jorgen (Knivsta SEX), Characterizing macromolecules interacting with at least three ligands on a sensor.
Janata Jiri (2231 Logan Ave. Salt Lake City UT 84108) Huber Robert J. (1145 E. Millbrook Way Bountiful UT 84010) Thompson Michael (3642 S. 2500 E. Salt Lake City UT 84109), Chemical selective sensors utilizing admittance modulated membranes.
Brooks, Donald Elliott; Harris, Paul C.; Olal, Andrew D.; Xie, Zongcen Charles; Richards, Brian G., Compensation for non-specific signals in quantitative immunoassays.
Craig Alan R. (Wilmington DE) Frey William A. (Wilmington DE) Leflar Charles C. (Wilmington DE) Looney Catharine E. (Wilmington DE) Luddy Michael A. G. (West Chester PA), Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays.
Hewlins Michael J. E. (Penylan GBX) O\Dowd Thomas C. (Woodborough GBX) Davis Robert H. (Corntown GBX) Winterburn Peter J. (Cyncoed GBX), Detection of diamines in biological fluids.
Scott M. Clark ; Robert H. Suva ; Michael R. Kepron ; Stanislaw Barski, Jr. ; Erwin F. Workman, Jr., Device and method for integrated diagnostics with multiple independent flow paths.
Bogart Gregory R. (Berthoud CO) Moddel Garret R. (Boulder CO) Maul Diana M. (Thornton CO) Etter Jeffrey B. (Boulder CO), Devices and methods for detection of an analyte based upon light interference.
Bogart Gregory R. (Fort Collins CO) Moddel Garret R. (Boulder CO) Maul Diana M. (Thornton CO) Etter Jeffrey B. (Boulder CO), Devices for detection of an analyte based upon light interference.
Miller B. John (Boulder CO) Maul Diana M. (Thornton CO) Blessing James (Boulder CO) Crosby Mark (Niwot CO) Kelley Howard (Boulder CO), Devices for detection of an analyte based upon light interference.
Jou Yi-Her (Libertyville IL) Stroupe Stephen D. (Libertyville IL) Markese James J. (Libertyville IL), Devices for performing ion-capture binding assays.
Poto Edward M. ; Strahs Kenneth R. ; Conner Timothy J. ; Delahanty Francis T. ; Moorman Michael ; Wieck Henry, Disposable electronic diagnostic instrument.
Cox Daniel E. (Franklin MA) Abril Obsidiana (Cambridge MA) Bauminger Sara (Newton MA) Neri Bruce P. (N. Andover MA) Shinefeld Lisa (Lexington MA), Double capture assay method employing a capillary flow device.
Crismore William F. ; Surridge Nigel A. ; McMinn Daniel R. ; Bodensteiner Richard J. ; Diebold Eric R. ; Delk R. Dale ; Burke David W. ; Ho Jiaxiong Jason ; Earl Robert Kitchel ; Heald Brian A., Electrochemical biosensor test strip.
Robert W. Henkens ; John P. O'Daly ; Marek Wojciechowski ; Honghua Zhang ; Najih Naser ; R. Michael Roe ; Thomas N. Stewart ; Deborah M. Thompson ; Rebecca Sundseth ; Steven E. Wegner, Electrochemical detection of nucleic acid sequences.
Richard J. Massey ; Michael J. Powell ; Walter J. Dressick ; Jonathan K. Leland ; Janel K. Hino ; Mohindar S. Poonian ; Leopoldo Della Ciana, Electrochemiluminescent rhenium moieties.
Massey Richard J. (Rockville MD) Powell Michael J. (Gaithersburg MD) Dressick Walter J. (Gaithersburg MD) Leland Jonathan K. (Gaithersburg MD) Hino Janel K. (Arlington VA) Poonian Mohindar S. (Gaithe, Electrochemiluminescent rhenium moieties and methods for their use.
Vreeke Mark (14603 Cedar Point Houston TX 77070) Rocca Patrick (91 ; Chemin de Clres 76130 Mont-Saint-Aignan FRX), Electrode and method for the detection of an affinity reaction.
Nomura Toshiaki (Higashichikuma JPX) Yamada Tohru (Matsudo JPX), Electrode-separated piezoelectric crystal oscillator and method for measurement using the electrode-separated piezoelect.
Grundig Bernd,DEX ; Renneberg Ilka,DEX ; Strehlitz Beate,DEX ; Kopinke Holm,DEX, Enzymatic-electrochemical one-shot affinity sensor for the quantitative determination of analytes for aqueous media and affinity assay.
Evangelista Ramon A. (Scarborough CAX) Templeton Eva F. G. (Willowdale CAX) Pollak Alfred (Toronto CAX), Enzyme-amplified lanthanide chelate luminescence.
Frank Caruso DE; Rachel Anne Caruso DE; Edwin Donath DE; Helmuth Mohwald DE; Gleb Sukhorukov RU, Fabrication of multilayer-coated particles and hollow shells via electrostatic self-assembly of nanocomposite multilayers on decomposable colloidal templates.
Richard J. Massey ; Mark T. Martin ; Liwen Dong ; Ming Lu ; Alan Fischer ; Fabian Jameison ; Pam Liang ; Robert Hoch ; Jonathan K. Leland, Graphitic nanotubes in luminescence assays.
Blankenship Robert Mitchell ; Finch William Christopher ; Mlynar Lubomir ; Schultz Barbara Jean, Hollow sphere organic pigment for paper or paper coatings.
de Jaeger Nikolaas C. J. (Hove BEX) Monbaliu Marcel J. (Mortsel BEX) Noppe Marcus J. M. (Kalmthout BEX) Konings Frank J. (Antwerpen BEX), Immunoassay using colorable latex particles.
Cragle Linda K. (San Diego CA) Harris Paul C. (Yorba Linda CA) Lee Shih-Yun (San Marcos CA) Tung Ker-Kong (Carlsbad CA) Vodian Morton A. (Escondido CA), Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect.
Lee Jin P. (Troy MI) Salcedo F. Brad (Ann Arbor MI) Robins Martin F. (Troy MI), Immunoassays using multiple monoclonal antibodies and scavenger antibodies.
Markert-Hahn Christine (Seeshaupt DEX) Ofenloch-Haehnle Beatus (Wielenbach DEX) Hoess Eva (Starnberg DEX) Huber Erasmus (Finning DEX), Immunologically active conjugates and method for their preparation.
Tarcha Peter J. (Lake Villa IL) Donovan James J. (Waukegan IL) Wong Martin (Grayslake IL), Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
Hansen W. Peter (20 E. 35th St. ; Apt. 7G New York NY 10016) Cennerazzo Michael (9 Oak St. Weehauken NJ 07087), Light scatter-based immunoassay without particle self aggregation.
Stimpson Donald I. (Gurnee IL) Gordon Julian (Lake Bluff IL) Hoijer Joanell V. (Arlington Heights IL), Light scattering optical waveguide method for detecting specific binding events.
Pham Andrew A. ; Coassin Peter J. ; Harootunian Alec Tate ; Stylli Harry ; Tsien Roger Y., Low fluorescence assay platforms and related methods for drug discovery.
Bergstrm Jan (Blinge SEX) Lfaas Stefan (Uppsala SEX) Johnsson Bo (Storvreta SEX), Matrix coating for sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems.
Richard J. Massey ; Gary F. Blackburn ; Elizabeth W. Wilkins ; Haresh P. Shah, Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection.
McGeehan John K. (Woodbury NJ) Ertingshausen Gerhard (Princeton NJ), Method and device for metering of fluid samples and detection of analytes therein.
Tom-Moy May (San Carlos CA) Myerson Joel (Berkeley CA) Robotti Karla M. (Foster City CA), Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic.
Sutherland Ranald M. (Geneva CHX) Dhne Claus (Onex CHX) Revillet Georges (Onex CHX), Method for optically ascertaining parameters of species in a liquid analyte.
McDonald Charles J. (Midland MI) Chonde Yohannes (Midland MI) Cohrs William E. (Midland MI) MacWilliams Dalton C. (Alamo CA), Method for preparing hollow latexes.
Kuo Hai-Hang ; Meritt Lisa A., Method for the determination of analyte concentration in a lateral flow sandwich immunoassay exhibiting high-dose hook effect.
Robinson Grenville Arthur,GBX ; Attridge John Worthington,GBX ; Deacon Julie Karen,GBX ; Daniels Phelim Brinley,GBX ; Love Colin Andrew,GBX ; Thomson Aileen Margaret,GBX, Method of assay having calibration within the assay.
Jones Charles Elwood (Yardley PA) Aviles Rafael Gonzalez (Harleysville PA) Fasano David Michael (Maple Glen PA) Vogel Martin (Jenkintown PA), Method of improving ultraviolet radiation absorption of a composition.
Cozzette Stephen N. (Hightstown NJ) Davis Graham (Plainsboro NJ) Itak Jeanne (Hamilton NJ) Lauks Imants R. (Yardley PA) Mier Randall M. (Ottawa NJ CAX) Piznik Sylvia (Jackson NJ) Smit Nicolaas (Hight, Method of manufacturing a plurality of uniform microfabricated sensing devices having an immobilized ligand receptor.
Kuypers Martinus H. (Riethoven NLX) Steeghs Gerardus F. J. (Geldrop NLX) Brinkmann Egbert (Enschede NLX), Method of providing a substrate with a layer comprising a polyvinyl based hydrogel and a biochemically active material.
Glass Michael J. ; Coombs Jana ; Malmstrom Sharon L. ; Wu Linxian, Methods for detection and discrimination of multiple analytes using fluorescent technology.
Sheiness Diana K. (Bothell WA) Adams Trevor H. (Buckinghamshire GB2) Stamm Michael R. (Bothell WA) Cangelosi Gerard A. (Seattle WA) Britschgi Theresa B. (Seattle WA) Dix Connie K. (Arlington WA), Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples.
Bamdad Cynthia C. (Sharon MA) Sigal George B. (Arlington MA) Strominger Jack L. (Lexington MA) Whitesides George M. (Newton MA), Molecular recognition at surfaces derivatized with self-assembled monolayers.
Chan Emerson W. (Libertyville IL) Schulze Werner (Waukegan IL) Robey William G. (Libertyville IL) Braun Brian P. (Gurnee IL) Daluga Cynthia K. (Lindenhurst IL) Kapsalis Andreas A. (Evanston IL) Knigg, Multilayer solid phase immunoassay support and method of use.
Barbera-Guillem Emilio ; Nelson M. Bud ; Castro Stephanie L., Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system.
Bruno Alfredo Emilio,CHX ; Barnard Steven Mark ; Ehrat Markus,CHX ; Volkel Reinhard,CHX ; Nussbaum Philippe,CHX ; Herzig Hans Peter,CHX, Optical detection apparatus for chemical analyses of small volumes of samples.
Watanabe Noriko (Nara JPX) Hamada Hiroshi (Nara JPX) Funada Fumiaki (Yamatokoriyama JPX), Optical device having a microlens and a process for making microlenses.
Leiner Marco J. (Graz ATX) Weiss Leonie (Graz ATX) Wolfbeis Otto S. (Graz ATX), Optical sensor for determining at least one parameter in a liquid or gaseous sample.
Aylott Jonathan W. ; Chen-Esterlit Zoe ; Friedl Jon H. ; Kopelman Raoul ; Savvateev Vadim N. ; Shinar Joseph, Optical sensors and multisensor arrays containing thin film electroluminescent devices.
Lihme Allan Otto Fog,DKX ; B.o slashed.g-Hansen Thorkild Christian,DKX ; Nielsen Claus Schafer,DKX, Permeable hollow particles having an outer shell of mechanically rigid porous material.
Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA) Winkler James L. (Palo Alto CA) Holmes Christopher P. (Sunnyvale CA) Solas Dennis W. (San Francisco CA), Photolabile nucleoside and peptide protecting groups.
Ponomarev, Gelii Vasilevich; Yashunsky, Dmitry Vladimirovich; Meltola, Niko Jarmo Juhani; Soini, Aleksi Elias, Porphyrin compounds, their conjugates and assay methods based on the use of said conjugates.
Berger Michael (Penzberg DEX) Deger Arno (Seeshaupt DEX) Maier Josef (Weilheim DEX), Preparation of a solid phase matrix containing a bound specific binding pair.
Giesecke Henning (Leverkusen DEX) Wolf Gerhard D. (Dormagen DEX) Mller Hanns P. (Odenthal DEX) von Gizycki Ulrich (Leverkusen DEX) Wienkenhver Martin (Leverkusen DEX), Process for improving the adherency of metallic coatings deposited without current on plastic surfaces.
Lichtenhan Joseph D. (Palmdale CA) Gilman Jeffrey W. (Landcaster CA) Feher Frank J. (Costa Mesa CA), Process for preparation of polyhedral oligomeric silsesquioxanes and systhesis of polymers containing polyhedral oligome.
Choi Su B. (Seo-ku KRX) Jang Tae H. (Youseong-ku KRX) Yoo Jin N. (Youseong-ku KRX) Lee Chan H. (Youseong-ku KRX), Process for preparing emulsion polymers having a hollow structure.
Clark Malcolm C. (Cheadle GB2) Henshall John B. (Manchester GB2) Hart Derrick A. (Rochdale GB2), Process for the production of triaryl methane compounds.
Goldstein David Michael ; Labadie Sharada Shenvi ; Rotstein David Mark ; Sjogren Eric Brian ; Talamas Francisco Xavier, Pyrazole derivatives P-38 MAP kinase inhibitors.
Bergstrm Jan (Blinge SEX) Lfas Stefan (Uppsala SEX) Johnsson Bo (Storvreta SEX), Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems.
Kowalski Alexander (Glenside PA) Vogel Martin (Jenkintown PA) Blankenship Robert M. (Lansdale PA), Sequential heteropolymer dispersion and a particulate material obtainable therefrom, useful in coating compositions as a.
Benjamin J. Feldman ; Adam Heller ; Ephraim Heller ; Fei Mao ; Joseph A. Vivolo ; Jeffery V. Funderburk ; Fredric C. Colman ; Rajesh Krishnan, Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator.
Van Ness Jeffrey (Bothell WA) Petrie Charles R. (Woodinville WA) Tabone John C. (Bothell WA) Vermeulen Nicolaas M. J. (Woodinville WA), Solid supports for nucleic acid hybridization assays.
Brown ; III William E. (Grayslake IL) Safford Sarah E. (Libertyville IL) Clemens John M. (Gurnee IL), Solid-phase analytical device and method for using same.
Barrett Ronald W. (Sunnyvale CA) Pirrung Michael C. (Durham NC) Stryer Lubert (Stanford CA) Holmes Christopher P. (Sunnyvale CA) Sundberg Steven A. (San Francisco CA), Spatially-addressable immobilization of anti-ligands on surfaces.
Barrett Ronald W. (Sunnyvale CA) Pirrung Michael C. (Durham NC) Stryer Lubert (Stanford CA) Holmes Christopher P. (Sunnyvale CA) Sundberg Steven A. (San Francisco CA), Spatially-addressable immobilization of anti-ligands on surfaces.
Malmqvist Magnus (Upsala SEX) Karlsson Robert (Upsala SEX) Rnnberg Inger (Upsala SEX), Surface plasmon resonance sensor unit and its use in biosensor systems.
Davidson Robert S. (496 Uppingham Road Leicester ; LE5 6QB GB2), Time-resolved luminescence binding assays using a fluorescent transition metal label other than ruthenium.
Klainer Stanley M. ; Coulter Stephen L. ; Hewitt Geoffrey F., Ultrasensitive single-step, solid-state competitive immunoassay sensor with interference modifier and/or gel layer.
David A. Zarling ; Michel J. Rossi CH; Norman A. Peppers ; James Kane ; Gregory W. Faris ; Mark J. Dyer ; Steve Y. Ng ; Luke V. Schneider, Up-converting reporters for biological and other assays using laser excitation techniques.
Zarling David A. (Menlo Park CA) Rossi Michel J. (Lausanne CHX) Peppers Norman A. (Belmont CA) Kane James (Lawrenceville NJ) Faris Gregory W. (Menlo Park CA) Dyer Mark J. (San Jose CA) Ng Steve Y. (S, Up-converting reporters for biological and other assays using laser excitation techniques.
Sagner Gregor,DEX ; De Haas Richard Robert,NLX ; Van Gijlswijk Rob,NLX ; Tanke Hans,NLX, Use of metallo-porphyrin conjugates for the detection of biological substances.
Rosann M. Kaylor ; Abraham B. Choi ; Chibueze Obinna Chidebelu-Eze ; Michael Heinrich Herbert Grunze DE, Use of wicking agent to eliminate wash steps for optical diffraction-based biosensors.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.