IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0902114
(2010-10-11)
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등록번호 |
US-8735846
(2014-05-27)
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발명자
/ 주소 |
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출원인 / 주소 |
- Advanced Technology Applications, LLC
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대리인 / 주소 |
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인용정보 |
피인용 횟수 :
1 인용 특허 :
23 |
초록
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A system, method, device, and process for making and using an electromagnetic-sensitive biosensor on a biosensor disk to identify and classify an analyte in a sample. The biosensor of the biosensor disk is exposed to a sample containing analytes and a desired analyte adheres to the biosensor. The bi
A system, method, device, and process for making and using an electromagnetic-sensitive biosensor on a biosensor disk to identify and classify an analyte in a sample. The biosensor of the biosensor disk is exposed to a sample containing analytes and a desired analyte adheres to the biosensor. The biosensor is exposed to microspheres that adhere to the analyte. The microspheres cause a detectable change to electromagnetic radiation incident upon the biosensor disk The biosensor disk is rotated during operation and an electromagnetic emitter directs an electromagnetic radiation beam at the biosensor disk. The returned electromagnetic radiation from the biosensor disk is received by a sensor and converted into a signal to indicate the presence of the desired analyte in the sample.
대표청구항
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1. A system for detecting one or more analytes in a sample using a biosensor, comprising: a biosensor disk, with an outer surface and a layer encoded with a data path capable of being read by an electromagnetic radiation incident upon said layer, said data path encoded with a baseline data that is s
1. A system for detecting one or more analytes in a sample using a biosensor, comprising: a biosensor disk, with an outer surface and a layer encoded with a data path capable of being read by an electromagnetic radiation incident upon said layer, said data path encoded with a baseline data that is static;a detector chamber disposed along said data path, said detector chamber having a surface for affixing detector ligands, said surface distinct from said layer encoded with said data path;a detector ligand for binding with an analyte, said detector ligand affixed to said surface for affixing detector ligands of said detector chamber;a detection enhancement means for binding to said analyte, wherein said detection enhancement means causes a detectable change to said electromagnetic radiation; anda disk system adapted to accept and rotate said biosensor disk, comprising a source of said electromagnetic radiation focused on said layer encoded with said data path of said biosensor disk, and a sensor adapted to detect said electromagnetic radiation returned from said layer encoded with said data path of said biosensor disk and convert said electromagnetic radiation into an electrical signal. 2. The system of claim 1, wherein said data path is encoded with a baseline data to provide a continuous discriminating signal, and said detector chamber is disposed on said data path such that said detection enhancement means, when bound to said analyte, changes said electromagnetic radiation returned from said biosensor disk such that a detectable signal change occurs in said electronic signal compared to said electronic signal produced by said baseline data. 3. The system of claim 1, further comprising: a threshold circuit to quantize said electrical signal into a binary representation of said electrical signal; anda digital circuit to construct a digital word from said binary representation and further comprising a CIRC encoder, a first error correction stage, and a second error correction stage that construct a decoded frame from multiple said digital words and outputs errors from said first error correction stage and said second error correction stage. 4. The system of claim 3, wherein said detector chamber is disposed on said data path such that said detection enhancement means, when bound to said analyte, changes said electromagnetic radiation returned from said biosensor disk such that a detectable signal change occurs in said electronic signal compared to said electronic signal produced by said baseline data, and said detectable signal change is selected from the group consisting of: an increase in said output errors from said first correction stage, an increase in said output errors from said second correction stage, an increase in said output errors from said first correction stage and said second correction stage, a change in said information of said decoded frame, and an unrecoverable error that results in said decoded frame being improperly reconstructed. 5. The system of claim 1, wherein said detector chamber is disposed on said outer surface of said biosensor disk and said detector chamber is orientated between said source of electromagnetic radiation and said data path. 6. The system of claim 1, wherein said detector chamber is disposed on a separate detector substrate fabricated from a material selected from the group consisting of a polymer, a silicon substrate, a doped silicon substrate, and substrate adapted to have an index of refraction approximately the same as said biosensor disk, and wherein said detector chamber further comprises a mating feature adapted to interlock with a corresponding feature on said biosensor disk to align said detector substrate with said data path. 7. The system of claim 1, wherein said detection enhancement means is selected from the group consisting of a biotinylated microsphere, a Streptavidin-coated microsphere, a microsphere having a diameter between approximately 1 μm and 10 μm, a polystyrene microsphere, a latex microsphere, a biotinylated microsphere that has been immersed in a solution comprising detector ligands, a Streptavidin-coated microsphere that has been immersed in a solution comprising detector ligands, a microsphere having a detector ligand, and a staining agent. 8. The system of claim 1, wherein said detection enhancement means comprises a staining agent selected from the group consisting of crystal violet, iodine, carbol fuchsin, methylene blue, malachite green, coomassie blue, bismarck brown, osmium tetraoxide, fuchsin, and safranin counterstain. 9. The system of claim 1, wherein said detector ligands are attached to said detector chamber using a micropatterning technique selected from the group consisting of photochemical micropatterning, micro-contact printing, micro-fluid network urging of detector ligands in a pattern, spotting detector ligands onto said detector chamber, and spraying detector ligands onto said detector chamber. 10. The system of claim 1, wherein said detector ligand is selected from the group consisting of a streptavidin receptor, an avidin-biotin receptor, a peptide, an olglionucleotide, cDNA, and a chelating agent. 11. A system for detecting one or more analytes in a sample using a biosensor, comprising: a biosensor disk, further comprising: an outer surface;a layer encoded with a data path encoded with a baseline data that is static and capable of being read by an electromagnetic radiation incident upon said layer; andan interlocking feature for accepting a detector substrate and aligning said detector substrate with said data path;a detector substrate having a mating feature for interlocking with said biosensor disk;a detector ligand adapted to bind with an analyte, said detector ligand affixed to said detector substrate;a coated microsphere adapted to bind with said analyte, wherein said coated microsphere bound to said analyte causes a detectable change to said electromagnetic radiation; anda disk system adapted to accept and rotate said biosensor disk, comprising a source of said electromagnetic radiation directed toward said layer encoded with said data path, and a sensor adapted to detect said electromagnetic radiation returned from said data path and convert said electromagnetic radiation into an electrical signal. 12. The system of claim 11, wherein said coated microsphere is selected from the group consisting of a biotinylated microsphere, a Streptavidin-coated microsphere, a microsphere having a diameter between approximately 1 μm and 10 μm, a polystyrene microsphere, a latex microsphere, a biotinylated microsphere that has been immersed in a solution comprising detector ligands, a Streptavidin-coated microsphere that has been immersed in a solution comprising detector ligands, and a microsphere having a detector ligand. 13. The system of claim 11, wherein said detector ligand is selected from the group consisting of an streptavidin receptor, an avidin-biotin receptor, a peptide, an olglionucleotide, cDNA, and a chelating agent. 14. The system of claim 11, wherein said detector substrate is fabricated from a material selected from the group consisting of: a polymer substrate, a silicon substrate, a doped silicon substrate, and substrate adapted to have an index of refraction approximately the same as said biosensor disk. 15. The system of claim 11, wherein said detector substrate further comprises multiple zones of detector ligands, each zone having detector ligands adapted to bind with at least one analyte, and wherein said microsphere is coated with a plurality of detector ligands associated with at least two zones. 16. The system of claim 11, further comprising a staining agent applied to said detector substrate to enhance detection of analytes by said disk system. 17. A method for detecting an analyte in a sample, comprising: (a) introducing the sample onto a detector substrate of a biosensor disk, wherein said biosensor disk is comprised of a layer having a data path encoded with a baseline data that is static, a detector chamber disposed along said data path, said detector chamber having a surface for affixing detector ligands that is distinct from said layer encoded with said data path, and a detector substrate adapted to interconnect with said detector chamber, said detector substrate further comprising a detector ligand adapted to bind with the analyte, said detector ligand affixed to said surface for affixing detector ligands of said detector chamber;(b) binding the analyte to said detector ligand disposed on said detector substrate to create a bound detector ligand;(c) introducing a coated microsphere onto said detector substrate;(d) binding said coated microsphere to said bound detector ligand, whereby said coated microsphere creates a detectable change to an electromagnetic radiation incident upon said coated microsphere;(e) interconnecting said detector substrate to said detector chamber of said biosensor disk;(f) placing said biosensor disk in a disk system;(g) rotating said biosensor disk with said disk system;(h) emitting said electromagnetic radiation from said disk system focused on said layer encoded with said data path on said biosensor disk;(i) receiving a returned electromagnetic radiation from said layer encoded with said data path of said biosensor disk; and(j) interpreting a change in said returned electromagnetic radiation caused by said detectable change to indicate the presence of the analyte. 18. The method of claim 17, wherein said coated microsphere is coated with a detector ligand adapted to bind with said analyte. 19. The method of claim 17, wherein said (c) introducing said coated microsphere is accomplished by introducing a solution containing a plurality of coated microspheres onto said detector substrate. 20. The method of claim 17, wherein said operations (c) and (d) are: (c) introducing a staining agent onto said bound detector ligand; and(d) binding said staining agent to said bound detector ligand, whereby said staining agent and said bound detector ligand create a detectable change to an electromagnetic radiation incident upon said coated microsphere.
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