Cell-free tissue replacement for tissue engineering
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61F-002/00
A61L-027/36
출원번호
US-0135772
(2008-06-09)
등록번호
US-8758794
(2014-06-24)
발명자
/ 주소
Schmidt, Christine
Hudson, Terry
출원인 / 주소
Board of Regents, The University of Texas System
대리인 / 주소
Flores, Edwin S.
인용정보
피인용 횟수 :
1인용 특허 :
25
초록▼
The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solution
The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and/or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.
대표청구항▼
1. A method for preparing a native, acellular nerve tissue replacement comprising the steps of: obtaining a nerve tissue;soaking the nerve tissue for at least six hours in a solution comprising one or more sulfobetaines;treating the nerve tissue in a mixture of one or more sulfobetaines and an anion
1. A method for preparing a native, acellular nerve tissue replacement comprising the steps of: obtaining a nerve tissue;soaking the nerve tissue for at least six hours in a solution comprising one or more sulfobetaines;treating the nerve tissue in a mixture of one or more sulfobetaines and an anionic sulfonate detergent; andwashing the nerve tissue in one or more solutions of a buffered salt to remove the excess anionic sulfonate detergent to form the native, acellular nerve tissue replacement that is immunologically tolerated when implanted. 2. The method of claim 1, further comprising the step of storing the native, acellular nerve tissue replacement in a buffered salt solution until needed. 3. The method of claim 1, wherein the sulfobetaines have hydrophilic tails of 10 to 16 carbons. 4. The method of claim 1, further comprising the step of adhering one or more bioactive agents to the nerve tissue replacement. 5. The method of claim 1, wherein the native, acellular nerve tissue comprises mammalian tissue. 6. The method of claim 4, wherein the one or more bioactive agents comprises a drug. 7. The method of claim 1, wherein the native, acellular nerve tissue replacement further comprises a structure selected from the group consisting of a tube, a sheet, a film, a scaffold, and a tissue transplant for delivery into the body. 8. The method of claim 1, wherein the sulfobetaine comprises SB-16. 9. The method of claim 1, wherein the anionic sulfonate detergent is Triton X-200. 10. The method of claim 1, wherein the step of washing the nerve tissue comprises one or more washes in a buffered salt solution comprising 100 mM sodium and 50 mM phosphate for at least 15 minutes each. 11. The method of claim 5, wherein the nerve tissue is harvested from a mammalian cadaver. 12. The method of claim 11, wherein the nerve tissue is cleaned of fat and blood after harvesting and rinsed two or more times in deionized distilled water. 13. The method of claim 1 wherein basal laminae and endoneurium layer of the native, acellular nerve tissue replacement substantially retain the native extracellular matrix structure. 14. The method of claim 1, wherein the native, acellular nerve tissue replacement, when implanted, elicits a T-cell mediated immune response that is less than an immune response triggered by an allogeneic implant. 15. The method of claim 1, wherein the native, acellular nerve tissue replacement allows for higher axon density when implanted relative to an untreated nerve tissue graft. 16. The method of claim 1 further comprising a step of: implanting the native, acellular nerve tissue into a mammal. 17. A method for preparing a native, acellular nerve tissue replacement comprising the steps of: obtaining a nerve tissue;soaking the nerve tissue for at least six hours in a solution comprising one or more sulfobetaines;treating the nerve tissue in a mixture of one or more sulfobetaines and a detergent selected from the group consisting of polyether sulfonate sold under the trademark TRITON X-200, sulfosuccinate, polyether phosphate ester, alkoxylate sulfate, sodium deoxycholate, and low foam; andwashing the nerve tissue in one or more solutions of a buffered salt to remove the excess detergent to form the native, acellular nerve tissue replacement that is immunologically tolerated when implanted.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (25)
Emil A. Tanagho ; Rajvir Dahiya ; Tom F. Lue ; Gerald R. Cunha, Acellular matrix grafts: preparation and use.
Livesey Stephen A. (Eltham TX AUX) del Campo Anthony A. (Houston TX) Nag Abhijit (Houston TX) Nichols Ken B. (The Woodlands TX) Coleman Christopher (Houston TX), Method for processing and preserving collagen-based tissues for transplantation.
Orgill Dennis P. ; Butler Charles E. ; Barlow Mark ; Ritterbush Scott ; Yannas Ioannis V. ; Compton Carolyn C., Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft.
Fishman Mark C. (Newton Centre MA) Igarashi Michihiro (Saitama JPX) Strittmatter Stephen M. (Clinton CT), Peptides to overcome inhibition of nerve growth.
Wolfinbarger, Jr., Lloyd; Lange, Perry; Linhurst, Alyce; Moore, Eric; Nolf, Barry, Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced.
Matsuda Takehisa,JPX ; Sugawara Takashi,JPX ; Inoue Kazuhiko,JPX, Substrate for controlling growth direction of nerve fibers and process for preparing the same, method for controlling g.
Bret A. Ferree, Treating degenerative disc disease through transplantion of extracellular nucleus pulposus matrix and autograft nucleus pulposus cells.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.