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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0515003 (2007-11-14) |
등록번호 | US-8765076 (2014-07-01) |
국제출원번호 | PCT/US2007/084730 (2007-11-14) |
§371/§102 date | 20100317 (20100317) |
국제공개번호 | WO2008/061165 (2008-05-22) |
발명자 / 주소 |
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출원인 / 주소 |
|
대리인 / 주소 |
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인용정보 | 피인용 횟수 : 27 인용 특허 : 612 |
The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising s
The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
1. A method of making a micro fluidic valve, the method of making comprising: directing a dispensing head over an inlet hole in a microfluidic substrate;propelling a quantity of thermally responsive substance from the dispensing head into the inlet hole; andmaintaining a temperature of the microflui
1. A method of making a micro fluidic valve, the method of making comprising: directing a dispensing head over an inlet hole in a microfluidic substrate;propelling a quantity of thermally responsive substance from the dispensing head into the inlet hole; andmaintaining a temperature of the microfluidic substrate so that the thermally responsive substance flows by capillary action from the inlet hole past a shoulder and into a microfluidic channel in communication with the inlet hole via the shoulder, wherein the shoulder is disposed between the inlet hole and the microfluidic channel. 2. The method of claim 1, wherein the quantity is 75±15 nl. 3. The method of claim 1, wherein the propelling utilizes compressed gas. 4. The method of claim 1, wherein the thermally responsive substance is wax. 5. The method of claim 1, wherein the shoulder is disposed at the bottom of the inlet hole. 6. The method of claim 1, further comprising maintaining a temperature of the thermally responsive substance in the dispensing head so that the thermally responsive substance is dispensed in molten form into the inlet. 7. The method of claim 1, wherein the volume of the microfluidic channel does not exceed a maximum volume of thermally responsive substance. 8. The method of claim 1, wherein the directing is automatically controlled. 9. The method of claim 1, wherein the quantity of thermally responsive substance is dispensed as a dot. 10. The method of claim 9, wherein the dot has one dimension of around 100 μm. 11. The method of claim 1, wherein the propelling utilizes heated air. 12. The method of claim 1, wherein the dispensing head is heated. 13. The method of claim 1, wherein microfluidic channel comprises a first end and a second end, and wherein the first end of the microfluidic channel is fluidly connected with the inlet hole. 14. The method of claim 1, wherein microfluidic channel comprises a first end and a second end, and wherein the second end of the micro fluidic channel intersects a flow channel. 15. The method of claim 14, wherein the intersection of the mlcrofluidic channel and the flow channel forms a T-junction which gives rise to lower capillary forces than in the microfluidic channel. 16. The method of claim 15, wherein the flow of the thermally responsive substance stops when the thermally responsive substance reaches the flow channel. 17. A microfluidic valve made by the method of claim 1. 18. A microfluidic substrate comprising one or more microfluidic valves made by the method of claim 1.
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