Methods for modulating osteochondral development using bioelectrical stimulation
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/00
C12N-005/02
출원번호
US-0904873
(2010-10-14)
등록번호
US-8785196
(2014-07-22)
발명자
/ 주소
Kronberg, James W.
Ganey, Timothy
Gordon, Stephen L.
출원인 / 주소
MedRelief Inc.
대리인 / 주소
Seed IP Law Group PLLC
인용정보
피인용 횟수 :
1인용 특허 :
58
초록▼
Compositions and methods are provided for modulating the growth, development and repair of bone, cartilage or other connective tissue. Devices and stimulus waveforms are provided to differentially modulate the behavior of osteoblasts, chondrocytes and other connective tissue cells to promote prolife
Compositions and methods are provided for modulating the growth, development and repair of bone, cartilage or other connective tissue. Devices and stimulus waveforms are provided to differentially modulate the behavior of osteoblasts, chondrocytes and other connective tissue cells to promote proliferation, differentiation, matrix formation or mineralization for in vitro or in vivo applications. Continuous-mode and pulse-burst-mode stimulation of cells with charge-balanced signals may be used. Bone, cartilage and other connective tissue growth is stimulated in part by nitric oxide release through electrical stimulation and may be modulated through co-administration of NO donors and NO synthase inhibitors. Bone, cartilage and other connective tissue growth is stimulated in part by release of BMP-2 and BMP-7 in response to electrical stimulation to promote differentiation of cells. The methods and devices described are useful in promoting repair of bone fractures, cartilage and connective tissue repair as well as for engineering tissue for transplantation.
대표청구항▼
1. A kit for preparing a tissue suitable for transplantation, the kit comprising: cells; andan electrical stimulator providing an electrical stimulus waveform that includes a number of consecutive D-type signal pulse bursts that repeat at a fixed interval of 1 second or longer between consecutive pu
1. A kit for preparing a tissue suitable for transplantation, the kit comprising: cells; andan electrical stimulator providing an electrical stimulus waveform that includes a number of consecutive D-type signal pulse bursts that repeat at a fixed interval of 1 second or longer between consecutive pulse bursts; wherein each of the number of consecutive D-type signal pulse bursts includes a D-type signal having a frequency of less than 910 Hz, a shorter negative voltage pulse length of between 100 microseconds (μsec) and 1000 μsec in duration, a longer positive voltage pulse length in excess of 1 millisecond in duration, and a pulse burst length of from about 100 milliseconds to about 1 second; andwherein the electrical stimulus waveform promotes proliferation, differentiation, or matrix production of the cells into a tissue suitable for transplantation. 2. The kit of claim 1 further comprising a biodegradable or biostable scaffold. 3. The kit of claim 2 wherein the scaffold is made from a material selected from natural or synthetic polymers. 4. The kit of claim 2 wherein the scaffold is in association with growth-promoting or adhesion-promoting molecules. 5. The kit of claim 1 further comprising means for mechanical loading of the cells. 6. The kit of claim 1 wherein each of the number of D-type signal pulse bursts has a duration of 1 second and comprises: a number of pulses at a frequency of approximately 76 Hz, wherein each of the number of pulses includes: a positive segment at a first voltage level, the positive segment having a longer duration (δ) equal to 13 milliseconds; anda negative segment at a second voltage level, the negative segment having a shorter duration (γ) equal to 200 microseconds; anda single equalizing pulse at the second voltage level. 7. The kit of claim 1 wherein the electrical stimulator is further configured to generate a second electrical signal including at least one of an A-type signal, a B-type signal, or a C-type signal, the second electrical signal having a first repeating pattern of a number of pulse bursts characteristic of at least one of an A-type signal, a B-type signal, or a C-type signal, each of the pulse bursts comprising a plurality of individual positive pulses of a first voltage level of a longer duration, the individual positive pulses separated by a number of negative pulses of a shorter duration at a second voltage level, each of the pulse bursts of a burst duration and separated from each other at least by an equalizing pulse at the second voltage level, where the longer duration, the shorter duration, and the burst duration are selected from the group consisting of: A-type signal) the longer duration of a length β between 20 μsec and 100 μsec, the shorter duration of a length a between 5 μsec and 75 μsec, and the burst duration between 0.5 msec and 500 msec;B-type signal) the longer duration of a length γ between 100 μsec and 1000 μsec, the shorter duration of a length a between 5 μsec and 75 μsec, the burst duration between 1.0 msec and 50 msec; andC-type signal) the longer duration of a length γ between 100 μsec and 1000 μsec, the shorter duration of a length 0 between 20 μsec and 100 μsec, and the burst duration between 1.0 msec and 50 msec.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (58)
Winder Alan A. (Westport CT) Talish Roger J. (West Caldwell NJ) Ryaby John P. (West Caldwell NJ), Acoustic system for bone-fracture therapy.
Maurer Donald D. (Marine on St. Croix MN) Kalm Michael L. (St. Louis Park MN) Kipnis Alexander (New Hope MN) Agarwala Poonam (New Brighton MN), Electronic pain feedback system and method.
Ryaby John P. (Essex Fells NJ) Pilla Arthur A. (Wyckoff NJ), Modification of the growth, repair and maintenance behavior of living tissues and cells by a specific and selective chan.
William Paul Kornrumpf ; Shankara Bonthu Reddy ; David Anthony Lovejoy ; Donald Eugene Brodnick, Multiple electrode assembly with extendible electrodes and methods of fabrication and application.
Bastyr Charles A. (12476 Rue Fountain Bleau San Diego CA 92131) Kolen Paul T. (7212 Linden Ter. Carlsbad CA 92009), Nerve stimulating device and associated support device.
Unsworth, John Duncan; Hirsh, Jack; Kaplan, Robert Edward; Czyrny, James Joseph, Neuromuscular electrical stimulation of the foot muscles for prevention of deep vein thrombosis and pulmonary embolism.
Liss Saul (Glen Rock NJ) Liss Bernard (Glen Rock NJ) Manor Ben (Glen Rock NJ), Non-intrusive analgesic neuroaugmentive apparatus and management system.
Pohl Jeff K. (Chattanooga TN) Johnson David A. (Signal Mountain TN) Dunlay Edward R. (Harrison TN), Physical therapy apparatus having an interactive interface, and method of configuring same.
Brighton,Carl T.; Carlozzi,Michael D., Portable electrotherapy device for treating osteoarthritis and other diseases, defects and injuries of the knee joint.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.