Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12P-019/34
출원번호
US-0874697
(2013-05-01)
등록번호
US-8802373
(2014-08-12)
발명자
/ 주소
Barany, Francis
Lubin, Matthew
Barany, George
Hammer, Robert
Belgrader, Phillip
출원인 / 주소
Cornell Research Foundation, Inc.
대리인 / 주소
LeClairRyan, a Professional Corporation
인용정보
피인용 횟수 :
3인용 특허 :
101
초록▼
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chai
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.
대표청구항▼
1. A method comprising: providing a sample potentially containing one or more target nucleotide sequences;forming primary products in a reaction process comprising a polymerase reaction, wherein each primary product comprises a 5′ primer-specific portion, a target nucleotide sequence-specific portio
1. A method comprising: providing a sample potentially containing one or more target nucleotide sequences;forming primary products in a reaction process comprising a polymerase reaction, wherein each primary product comprises a 5′ primer-specific portion, a target nucleotide sequence-specific portion, and a 3′ primer-specific portion, wherein the 5′ primer-specific portion and the 3′ primer-specific portion differ from each other and are not the same as or complementary to the target-specific portion;providing one or more oligonucleotide primer sets, each oligonucleotide primer set characterized by a first and second oligonucleotide primer, wherein said first oligonucleotide primer is capable of hybridizing to the 3′ primer-specific portion of a primary product and said second oligonucleotide primer is capable of hybridizing to a 3′ primer specific portion of a complement of the primary product; andforming secondary products in a polymerase reaction mixture comprising the one or more oligonucleotide primer sets, wherein said secondary products are complementary to each of the formed primary products or complements thereof. 2. The method of claim 1 further comprising: sequencing the secondary products. 3. The method of claim 1 further comprising: coupling a label to the secondary products anddetecting the label coupled to the secondary products formed in the polymerase chain reaction mixture. 4. The method of claim 3 further comprising: distinguishing, based on said detecting, two or more target nucleotide sequences in the sample that differ by one or more single-base changes, insertions, deletion, or translocations. 5. The method of claim 3 further comprising: sequencing, based on said detecting, the secondary products. 6. The method of claim 1, wherein the primary products comprise a further portion. 7. The method of claim 6, wherein the further portion of one primary product differs from the further portion of a different primary product. 8. The method of claim 1, wherein the secondary products are immobilized on a solid support. 9. A method comprising: providing a sample potentially containing one or more target nucleotide sequences and complements thereof;adding a 5′ primer-specific portion and a 3′ primer-specific portion, which differ from each other, to the one or more target nucleotide sequences and complements thereof in the sample in a reaction process comprising a polymerase reaction to form one or more primer labeled target nucleotide sequences and complements thereof; andforming extension products that are complementary to the one or more primer labeled target nucleotide sequences and complements thereof in a polymerase reaction mixture comprising one or more oligonucleotide primer sets, each oligonucleotide primer set characterized by a first and second oligonucleotide primer, wherein said first oligonucleotide primer is capable of hybridizing to a 3′ primer-specific portion of a primer labeled target nucleotide sequence and said second oligonucleotide primer is capable of hybridizing to a 3′ primer specific portion of the complement of the primer labeled target nucleotide sequence. 10. The method of claim 9 further comprising: sequencing the extension products. 11. The method of claim 9 further comprising: coupling a label to the extension products anddetecting the label coupled to the extension products formed in the polymerase reaction mixture. 12. The method of claim 11 further comprising: distinguishing, based on said detecting, two or more target nucleotide sequences or complements thereof in the sample that differ by one or more single-base changes, insertions, deletion, or translocations. 13. The method of claim 11 further comprising: sequencing, based on said detecting, the extension products. 14. The method of claim 9, wherein the primer labeled target nucleotide sequences and complements thereof comprise a further portion. 15. The method of claim 14, wherein the further portion of one primer labeled target nucleotide sequence or complement thereof differs from the further portion of a different primer labeled target nucleotide sequence or complement thereof. 16. The method of claim 9, wherein the extension products are immobilized on a solid support.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (101)
Chakraborty Prasanta R. (Scotch Plains NJ) Elbrecht Alex (Watchung NJ) Dashkevicz Michael (Jamesburg NJ) Feighner Scott D. (Scotch Plains NJ) Liberator Paul A. (Jackson NJ) Profous-Juchelka Helen (St, Eimeria brunetti 16s rDNA probes.
Collier David Nash ; Ebersole Richard Calvin ; Hatfield Tina Marie ; Hendrickson Edwin R. ; Moran John Richard, Amplification of assay reporters by nucleic acid replication.
Bhatnagar Satish K. (Gaithersburg MD) George ; Jr. Albert L. (Gaithersburg MD) Nazarenko Irina (Gaithersburg MD), Amplification of nucleic acid sequences.
Zaun Peter (Libertyville IL) Bouma Stanley R. (Grayslake IL) Gordon Julian (Lake Bluff IL) Kotlarik John J. (Vernon Hills IL), Apparatus and method for amplifying and detecting target nucleic acids.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Array of oligonucleotides on a solid substrate.
McGall Glenn Hugh ; Miyada Charles Garrett ; Cronin Maureen T. ; Tan Jennifer Dee ; Chee Mark S., Arrays of modified nucleic acid probes and methods of use.
Chee Mark ; Cronin Maureen T. ; Fodor Stephen P. A. ; Huang Xiaohua X. ; Hubbell Earl A. ; Lipshutz Robert J. ; Lobban Peter E. ; Morris MacDonald S. ; Sheldon Edward L., Arrays of nucleic acid probes on biological chips.
Ekins Roger P. (Department of Molecular Endocrinology University College and Middlesex School of Medicine Mortimer Street London W1N 8AA GBX) Chu Frederick W. (Department of Molecular Endocrinology U, Binding assay employing labelled reagent.
Fraiser Melinda S. (Durham NC) Walker George T. (Chapel Hill NC) Schram James L. (Knightdale NC), Decontamination of nucleic acid amplification reactions using uracil-N-glycosylase (UDG).
Barany Francis ; Lubin Matthew ; Belgrader Phillip, Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions.
Barany, Francis; Gerry, Norman P.; Witowski, Nancy E.; Day, Joseph; Hammer, Robert P.; Barany, George, Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays.
Whiteley Norman M. (San Carlos CA) Hunkapiller Michael W. (San Carlos CA) Glazer Alexander N. (Orinda CA), Detection of specific sequences in nucleic acids.
Bergmeyer, Lynn; Cummins, Thomas J.; Findlay, John Bruce; Kerschner, JoAnne H., Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures.
Willis, Thomas D.; Hardenbol, Paul; Jain, Maneesh; Stolc, Viktor; Ronaghi, Mostafa; Davis, Ronald W., Direct multiplex characterization of genomic DNA.
Froehler Brian (Belmont CA) Matteucci Mark (Burlingame CA), Enhanced triple-helix and double-helix formation with oligomers containing modified purines.
Lockhart David J. ; Brown Eugene L. ; Wong Gordon G. ; Chee Mark S. ; Gingeras Thomas R., Expression monitoring by hybridization to high density oligonucleotide arrays.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of an array of polymers.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
Backman Keith C. (Bedford MA) Carrino John J. (Gurnee IL) Shimer George H. (Boston MA) Yocum Robert R. (Lexington MA), Ligase chain reaction with endonuclease IV correction and contamination control.
Leckie Gregor W. (Highland Park IL) Davis Alan H. (Vernon Hills IL) Semple-Facey Ingrid E. (Beach Park IL) Manlove Matthew T. (Vernon Hills IL) Solomon Natalie A. (Buffalo Grove IL), Materials and methods for the detection of Mycobacterium tuberculosis.
Matson Robert S. (Orange CA) Coassin Peter J. (San Juan Capistrano CA) Rampal Jang B. (Yorba Linda CA) Southern Edwin M. (Kidlington GB2), Method and apparatus for creating biopolymer arrays on a solid support surface.
Grossman Paul D. (Burlingame CA) Fung Steven (Palo Alto CA) Menchen Steven M. (Fremont CA) Woo Sam L. (Redwood City CA) Win-Deen Emily S. (Foster City CA), Method and probe composition for detecting multiple sequences in a single assay.
Davis Ronald W. (Palo Alto CA) Myles Arthur (Hopedale MA), Method for detecting a nucleotide at a specific location within a nucleic acid using exonuclease activity.
Ullman Edwin F. (Atherton CA) Goodman Thomas C. (Mountain View CA) Stull Paul D. (Mountain View CA), Method for detection of specific nucleic acid sequences.
Kinzler Kenneth W. (Bel Air MD) Vogelstein Bert (Baltimore MD) Velculescu Victor E. (Baltimore MD) Zhang Lin (Baltimore MD), Method for serial analysis of gene expression.
Drmanac Radoje T. (Zvecanska 46 Beograd 11000) Crkvenjakov Radomir B. (Bulevar JNA 118 Beograd YUX 11000), Method of sequencing of genomes by hybridization of oligonucleotide probes.
Orle Karina A. (San Francisco CA) Weiss Judith B. (Oakland CA), Methods and reagents for the detection of herpes simplex virus, treponema pallidum, and haemophilus ducreyi.
David J. Lockhart ; Mark Chee ; Kevin Gunderson ; Lai Chaoqiang ; Lisa Wodicka ; Maureen T. Cronin ; Danny Lee ; Huu M. Tran ; Hajime Matsuzaki, Nucleic acid analysis techniques.
Bouma Stanley R. (Mundelein IL) Joseph Jeffrey L. (Cherry Hill NJ) Marshall Ronald L. (Zion IL) Laffler Thomas G. (Libertyville IL), Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of h.
Chetverin Alexander B.,RUX ; Kramer Fred Russell, Oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids.
Burczak John D. (Highland Park IL) Carrino John J. (Gurnee IL) Klonowski Paul A. (Wonder Lake IL) Manlove Matthew T. (Vernon Hills IL) Marshall Ronald L. (Zion IL) Pabich Edward K. (Chicago IL) Salit, Oligonucleotides and methods for the detection of chlamydia trachomatis.
Grossman Paul D. (Burlingame CA) Fung Steven (Palo Alto CA) Menchen Steven M. (Fremont CA) Woo Sam L. (Redwood City CA) Winn-Deen Emily S. (Foster City CA), Probe composition containing a binding domain and polymer chain and methods of use.
Grossman Paul David ; Fung Steven ; Menchen Steven Michael ; Woo Sam Lee ; Winn-Deen Emily Susan, Probe composition containing a binding domain and polymer chain and methods of use.
Plikaytis Bonnie B. (Tucker GA) Shinnick Thomas M. (Atlanta GA) Crawford Jack T. (Dunwoody GA), Rapid amplification-based subtyping of mycobacterium tuberculosis.
Windle Bradford E. ; Qiu Ming ; Chen Shih-Fong ; Fletcher Terace M. ; Maine Ira, Rapid and sensitive assays for detecting and distinguishing between processive and non-processive telomerase activities.
Gelfand David H. (Oakland CA) Kwok Shirley Y. (San Ramon CA) Sninsky John J. (El Sobrante CA), Reduction of non-specific amplification glycosylase using DUTP and DNA uracil.
McGall Glenn H. (Mountain View CA) Fodor Stephen P. A. (Palo Alto CA) Sheldon Edward L. (Menlo Park CA), Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Synthesis and screening of immobilized oligonucleotide arrays.
Barany Francis ; Zebala John ; Nickerson Deborah ; Kaiser ; Jr. Robert J. ; Hood Leroy, Thermostable ligase mediated DNA amplification system for the detection of genetic diseases.
Barany Francis (New York NY) Zebala John (New York NY) Nickerson Deborah (Seattle WA) Kaiser ; Jr. Robert J. (Seattle WA) Hood Leroy (Seattle WA), Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease.
Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA), Very large scale immobilized polymer synthesis.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.